Strain Name:
C57BL/6J-MtgxR8535Btlr/Mmmh
Stock Number:
068504-MU
Citation ID:
RRID:MMRRC_068504-MU
Other Names:
R8535 (G1)
Major Collection:

Strain Information

Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: Todo, Gena 52, GENA 60, Dz, Cycn, Cyn, Obt, Mt, Whi, GENA 47, A730019I05Rik, Lda, Flo, Edy, WBE1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Dst
Name: dystonin
Synonyms: Macf2, A830042E19Rik, Bpag1, Bpag, bullous pemphigoid antigen 1, nmf203, athetoid, nmf339, BPAG1, BPAG1-n, 2310001O04Rik, bullous pemphigoid antigen 1, ah
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Map1b
Name: microtubule-associated protein 1B
Synonyms: Mtap1b, Mtap5, LC1, MAP5, Mtap-5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Dock5
Name: dedicator of cytokinesis 5
Synonyms: rlc, 1110060D06Rik, lr2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Yeats2
Name: YEATS domain containing 2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208146
VEGA: 16
Homologene: 9967
Pcnt
Name: pericentrin (kendrin)
Synonyms: m275Asp, Pcnt2, m239Asp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18541
VEGA: 10
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: trithorax Drosophila, ALL-1, Mll1, Cxxc7, Mll, HTRX1, All1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Ptpn21
Name: protein tyrosine phosphatase, non-receptor type 21
Synonyms: PTPD1, PTPRL10
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 24000
VEGA: 12
HGNC: HGNC:9651
Homologene: 5110
Vps50
Name: VPS50 EARP/GARPII complex subunit
Synonyms: Ccdc132, 1700034M03Rik, 8430415E05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73288
Homologene: 11498
Vav2
Name: vav 2 oncogene
Synonyms: 2810040F13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22325
Homologene: 2530
Taf4b
Name: TATA-box binding protein associated factor 4b
Synonyms: 105kDa, 4932409F03Rik, Taf2c2, 2610524B04Rik, TAFII105
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 72504
VEGA: 18
Homologene: 28266
Styxl2
Name: serine/threonine/tyrosine interacting like 2
Synonyms: C130085G02Rik, Dusp27
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240892
Homologene: 18949
Tmeff2
Name: transmembrane protein with EGF-like and two follistatin-like domains 2
Synonyms: 7630402F16Rik, 4832418D20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 56363
Homologene: 9417
Tnfrsf19
Name: tumor necrosis factor receptor superfamily, member 19
Synonyms: TAJ, TRADE, TAJ-ALPHA, Troy
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 29820
VEGA: 14
Homologene: 8481
Myh8
Name: myosin, heavy polypeptide 8, skeletal muscle, perinatal
Synonyms: Myhsp, Myhs-p, 4832426G23Rik, MyHC-pn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17885
HGNC: HGNC:7578
Homologene: 68256
Unc13c
Name: unc-13 homolog C
Synonyms: D9Ertd414e, Munc13-3, 1500037O19Rik, Unc13h3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 208898
Homologene: 45443
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20192
Homologene: 68151
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Crybg2
Name: crystallin beta-gamma domain containing 2
Synonyms: Aim1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230806
Homologene: 19232
Gm10750
Name: predicted gene 10750
Type: Gene
Species: Mouse
Chromosome: 2
Klhl25
Name: kelch-like 25
Synonyms: 2810402K13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 207952
Homologene: 32546
Fah
Name: fumarylacetoacetate hydrolase
Synonyms: swst
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14085
HGNC: HGNC:3579
Homologene: 110
Sardh
Name: sarcosine dehydrogenase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192166
Homologene: 5149
Pkn3
Name: protein kinase N3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 263803
Homologene: 50980
Wdr27
Name: WD repeat domain 27
Synonyms: 0610012K18Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 71682
VEGA: 17
Homologene: 18417
Scart1
Name: scavenger receptor family member expressed on T cells 1
Synonyms: E430002D04Rik, Cd163l1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244233
Homologene: 129778
Acsl6
Name: acyl-CoA synthetase long-chain family member 6
Synonyms: A330035H04Rik, Facl6, Lacsl
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216739
Homologene: 100939
Tcirg1
Name: T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3
Synonyms: V-ATPase a3, OC-116, Atp6i, TIRC7, ATP6a3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 27060
Homologene: 4392
Anks1b
Name: ankyrin repeat and sterile alpha motif domain containing 1B
Synonyms: AIDA-1b, LOC380650, E530015N03Rik, Gm10937, C030032C09Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77531
Homologene: 51570
Or4c127
Name: olfactory receptor family 4 subfamily C member 127
Synonyms: GA_x6K02T2Q125-51434523-51435437, MOR234-1, Olfr1262
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258976
Homologene: 74064
Tbcb
Name: tubulin folding cofactor B
Synonyms: 2410007D12Rik, Ckap1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66411
HGNC: HGNC:1989
Homologene: 981
Gcnt7
Name: glucosaminyl (N-acetyl) transferase family member 7
Synonyms: A330041C17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 654821
Homologene: 107308
Gja10
Name: gap junction protein, alpha 10
Synonyms: Cx59, connexin-57, Cx-57, Cx57
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14610
Homologene: 7733
Sh3bp5
Name: SH3-domain binding protein 5 (BTK-associated)
Synonyms: Sab
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 24056
Homologene: 23450
Trdv2-1
Name: T cell receptor delta variable 2-1
Synonyms: ENSMUSG00000068352
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100101942
Or6c200-ps1
Name: olfactory receptor family 6 subfamily C member 200, pseudogene 1
Synonyms: Gm6349, MOR115-6_p, Olfr227, GA_x6K02T2PULF-10720146-10719487, Olfr764, Olfr764-ps1, GA_x6K02T00F4V-833-1750
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 622733
Homologene: 67771
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,186,001 bp
  • T to A, chromosome 1 at 51,181,826 bp
  • T to C, chromosome 1 at 166,101,161 bp
  • T to A, chromosome 2 at 27,239,645 bp
  • G to A, chromosome 2 at 27,271,829 bp
  • A to T, chromosome 2 at 30,079,924 bp
  • G to A, chromosome 2 at 90,003,167 bp
  • A to G, chromosome 2 at 112,949,088 bp
  • A to G, chromosome 2 at 149,015,968 bp
  • T to C, chromosome 2 at 172,454,546 bp
  • T to A, chromosome 4 at 8,859,211 bp
  • G to A, chromosome 4 at 32,602,274 bp
  • T to C, chromosome 4 at 134,081,203 bp
  • T to C, chromosome 6 at 3,565,612 bp
  • G to A, chromosome 7 at 30,226,996 bp
  • G to A, chromosome 7 at 75,866,095 bp
  • A to T, chromosome 7 at 84,601,097 bp
  • G to A, chromosome 7 at 140,224,721 bp
  • A to C, chromosome 9 at 44,819,515 bp
  • G to A, chromosome 9 at 73,540,371 bp
  • G to A, chromosome 9 at 108,111,452 bp
  • G to A, chromosome 10 at 76,420,205 bp
  • T to A, chromosome 10 at 90,948,631 bp
  • T to C, chromosome 10 at 129,034,023 bp
  • A to T, chromosome 11 at 54,338,502 bp
  • G to A, chromosome 11 at 67,278,915 bp
  • T to A, chromosome 12 at 98,680,026 bp
  • T to A, chromosome 13 at 99,435,154 bp
  • A to G, chromosome 14 at 31,417,418 bp
  • T to A, chromosome 14 at 53,946,515 bp
  • T to C, chromosome 14 at 60,970,968 bp
  • A to G, chromosome 14 at 67,793,976 bp
  • C to T, chromosome 16 at 20,159,176 bp
  • G to A, chromosome 17 at 14,903,537 bp
  • T to A, chromosome 18 at 14,822,138 bp
  • A to G, chromosome 19 at 3,896,324 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8535 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068504-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.