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Strain Name:
C57BL/6J-MtgxR8559Btlr/Mmmh
Stock Number:
068522-MU
Citation ID:
RRID:MMRRC_068522-MU
Other Names:
R8559 (G1)
Major Collection:

Strain Information

Grin3a
Name: glutamate receptor ionotropic, NMDA3A
Synonyms: NMDAR-L, NR3A, A830097C19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242443
Homologene: 128613
Ccn2
Name: cellular communication network factor 2
Synonyms: Hcs24, hypertrophic chondrocyte-specific gene product 24, Fisp12, Ccn2, Ctgf
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14219
HGNC: HGNC:2500
Homologene: 1431
Vps54
Name: VPS54 GARP complex subunit
Synonyms: Vps54l, 5330404P15Rik, mSLP8, wr
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245944
Homologene: 5605
Clcn6
Name: chloride channel, voltage-sensitive 6
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26372
HGNC: HGNC:2024
Homologene: 985
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107182
Homologene: 31978
Zfp619
Name: zinc finger protein 619
Synonyms: 3000002G13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70227
Homologene: 133818
Inppl1
Name: inositol polyphosphate phosphatase-like 1
Synonyms: SHIP2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16332
HGNC: HGNC:6080
Homologene: 1204
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 4,349,561 bp
  • C to T, chromosome 1 at 10,408,349 bp
  • A to G, chromosome 1 at 46,725,139 bp
  • G to A, chromosome 1 at 150,676,038 bp
  • A to G, chromosome 1 at 151,818,418 bp
  • T to C, chromosome 1 at 172,251,330 bp
  • T to A, chromosome 2 at 66,287,733 bp
  • A to G, chromosome 2 at 84,989,336 bp
  • A to G, chromosome 2 at 86,228,644 bp
  • A to T, chromosome 2 at 88,865,183 bp
  • T to A, chromosome 2 at 89,440,184 bp
  • T to A, chromosome 2 at 155,992,736 bp
  • C to T, chromosome 2 at 158,541,801 bp
  • T to A, chromosome 3 at 90,263,597 bp
  • C to A, chromosome 4 at 49,770,555 bp
  • T to A, chromosome 4 at 120,600,811 bp
  • C to T, chromosome 4 at 148,026,575 bp
  • T to C, chromosome 5 at 31,256,577 bp
  • T to A, chromosome 5 at 65,825,285 bp
  • G to A, chromosome 6 at 3,692,603 bp
  • C to A, chromosome 6 at 8,166,681 bp
  • A to T, chromosome 6 at 92,807,136 bp
  • T to C, chromosome 6 at 131,685,425 bp
  • T to A, chromosome 7 at 39,537,135 bp
  • T to A, chromosome 7 at 66,282,327 bp
  • T to A, chromosome 7 at 86,166,272 bp
  • G to T, chromosome 7 at 101,829,627 bp
  • T to C, chromosome 8 at 124,542,908 bp
  • A to T, chromosome 9 at 82,025,631 bp
  • T to A, chromosome 9 at 108,575,394 bp
  • CCCGCCAACCGC to CCCGC, chromosome 10 at 24,596,068 bp
  • T to C, chromosome 10 at 56,388,208 bp
  • T to A, chromosome 10 at 89,732,186 bp
  • A to G, chromosome 10 at 128,670,731 bp
  • T to C, chromosome 11 at 21,264,815 bp
  • T to A, chromosome 11 at 64,981,676 bp
  • C to T, chromosome 11 at 87,604,124 bp
  • T to A, chromosome 11 at 87,864,792 bp
  • T to C, chromosome 11 at 97,537,149 bp
  • T to C, chromosome 11 at 101,156,784 bp
  • T to C, chromosome 11 at 104,584,104 bp
  • T to G, chromosome 11 at 121,250,432 bp
  • T to C, chromosome 12 at 4,871,471 bp
  • A to C, chromosome 12 at 13,352,808 bp
  • T to A, chromosome 12 at 16,696,435 bp
  • G to A, chromosome 12 at 80,475,857 bp
  • A to G, chromosome 12 at 103,197,253 bp
  • A to T, chromosome 12 at 103,593,161 bp
  • T to A, chromosome 13 at 15,660,132 bp
  • T to C, chromosome 14 at 52,149,257 bp
  • C to A, chromosome 14 at 70,777,528 bp
  • A to T, chromosome 15 at 35,876,642 bp
  • A to T, chromosome 15 at 80,212,169 bp
  • T to C, chromosome 15 at 102,001,544 bp
  • A to G, chromosome 16 at 3,965,282 bp
  • T to C, chromosome 16 at 5,064,770 bp
  • A to G, chromosome 16 at 26,712,134 bp
  • T to C, chromosome 16 at 32,754,715 bp
  • A to G, chromosome 17 at 19,812,384 bp
  • A to T, chromosome 17 at 37,903,828 bp
  • G to A, chromosome 18 at 57,240,627 bp
  • T to A, chromosome 18 at 61,155,419 bp
  • A to G, chromosome 19 at 36,986,873 bp
  • C to A, chromosome 19 at 55,210,354 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8559 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068522-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.