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Strain Name:
C57BL/6J-MtgxR8958Btlr/Mmmh
Stock Number:
068794-MU
Citation ID:
RRID:MMRRC_068794-MU
Other Names:
R8958 (G1)
Major Collection:

Strain Information

Zmpste24
Name: zinc metallopeptidase, STE24
Synonyms: A530043O15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230709
Homologene: 4277
Ptx3
Name: pentraxin related gene
Synonyms: TSG-14, pentraxin 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19288
HGNC: HGNC:9692
Homologene: 7500
Cr1l
Name: complement C3b/C4b receptor 1 like
Synonyms: mCRY, Crry
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12946
Homologene: 128275
Adck2
Name: aarF domain containing kinase 2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 57869
Homologene: 49103
E2f7
Name: E2F transcription factor 7
Synonyms: A630014C11Rik, D10Ertd739e, E2F7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52679
Homologene: 18685
Cltc
Name: clathrin heavy chain
Synonyms: CHC
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67300
HGNC: HGNC:2092
Homologene: 3572
Tcf3
Name: transcription factor 3
Synonyms: ALF2, E2A, E47, E12, Pan2, Pan1, A1, Tcfe2a, bHLHb21
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21423
Homologene: 2408
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 25,826,109 bp
  • C to T, chromosome 1 at 40,543,017 bp
  • A to T, chromosome 1 at 189,653,153 bp
  • C to G, chromosome 1 at 195,129,935 bp
  • A to C, chromosome 2 at 26,275,565 bp
  • T to C, chromosome 2 at 111,645,725 bp
  • C to T, chromosome 2 at 119,383,381 bp
  • T to A, chromosome 2 at 139,732,075 bp
  • T to A, chromosome 2 at 147,368,597 bp
  • T to C, chromosome 2 at 180,193,799 bp
  • G to A, chromosome 3 at 66,220,970 bp
  • T to C, chromosome 3 at 89,236,453 bp
  • T to C, chromosome 3 at 158,240,501 bp
  • A to G, chromosome 4 at 40,474,376 bp
  • A to G, chromosome 4 at 100,994,086 bp
  • A to G, chromosome 4 at 118,142,376 bp
  • C to A, chromosome 4 at 121,087,311 bp
  • A to T, chromosome 5 at 14,675,355 bp
  • A to G, chromosome 5 at 14,713,042 bp
  • A to G, chromosome 5 at 23,964,936 bp
  • C to A, chromosome 5 at 31,199,481 bp
  • T to C, chromosome 5 at 97,392,225 bp
  • T to C, chromosome 5 at 103,551,107 bp
  • T to C, chromosome 5 at 107,378,011 bp
  • A to G, chromosome 5 at 140,425,439 bp
  • T to C, chromosome 6 at 39,583,914 bp
  • C to T, chromosome 6 at 111,495,822 bp
  • G to C, chromosome 6 at 115,585,356 bp
  • C to A, chromosome 7 at 100,020,922 bp
  • T to C, chromosome 7 at 100,929,812 bp
  • T to C, chromosome 8 at 27,154,912 bp
  • A to T, chromosome 8 at 111,172,484 bp
  • T to C, chromosome 9 at 39,572,795 bp
  • G to T, chromosome 9 at 71,550,203 bp
  • A to T, chromosome 9 at 101,211,435 bp
  • A to G, chromosome 9 at 102,195,415 bp
  • A to T, chromosome 9 at 109,278,742 bp
  • T to C, chromosome 10 at 5,231,768 bp
  • T to C, chromosome 10 at 18,214,178 bp
  • T to G, chromosome 10 at 44,440,733 bp
  • G to T, chromosome 10 at 52,096,094 bp
  • T to C, chromosome 10 at 80,410,257 bp
  • C to A, chromosome 10 at 110,765,754 bp
  • C to T, chromosome 11 at 59,073,332 bp
  • C to G, chromosome 11 at 70,640,904 bp
  • A to T, chromosome 11 at 86,695,577 bp
  • A to T, chromosome 12 at 21,349,933 bp
  • T to C, chromosome 12 at 49,385,161 bp
  • G to A, chromosome 12 at 110,620,371 bp
  • T to C, chromosome 13 at 23,562,247 bp
  • T to A, chromosome 13 at 100,217,609 bp
  • A to T, chromosome 15 at 76,361,494 bp
  • G to A, chromosome 16 at 4,213,308 bp
  • T to A, chromosome 16 at 13,648,458 bp
  • A to G, chromosome 16 at 18,402,626 bp
  • T to C, chromosome 16 at 70,478,322 bp
  • T to A, chromosome 17 at 36,988,628 bp
  • T to C, chromosome 17 at 45,393,201 bp
  • T to C, chromosome 18 at 20,870,610 bp
  • T to A, chromosome 19 at 33,720,961 bp
  • A to T, chromosome 19 at 34,475,014 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8958 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068794-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.