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Strain Name:
C57BL/6J-MtgxR9163Btlr/Mmmh
Stock Number:
068975-MU
Citation ID:
RRID:MMRRC_068975-MU
Other Names:
R9163 (G1)
Major Collection:

Strain Information

Sim1
Name: single-minded family bHLH transcription factor 1
Synonyms: bHLHe14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20464
VEGA: 10
Homologene: 3715
Gldc
Name: glycine decarboxylase
Synonyms: D19Wsu57e, D030049L12Rik, b2b2679Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Upf1
Name: UPF1 RNA helicase and ATPase
Synonyms: PNORF-1, B430202H16Rik, Rent1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19704
HGNC: HGNC:9962
Homologene: 2185
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Xrn1
Name: 5'-3' exoribonuclease 1
Synonyms: mXrn1, Dhm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24127
Homologene: 5894
Scrib
Name: scribbled planar cell polarity
Synonyms: Scrb1, Crc
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105782
Homologene: 44228
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 74,394,967 bp
  • A to G, chromosome 1 at 96,836,908 bp
  • A to G, chromosome 1 at 161,949,605 bp
  • A to T, chromosome 1 at 174,017,422 bp
  • A to T, chromosome 2 at 69,776,166 bp
  • A to T, chromosome 2 at 76,870,427 bp
  • C to T, chromosome 2 at 88,865,451 bp
  • G to A, chromosome 2 at 103,022,216 bp
  • A to T, chromosome 2 at 167,056,341 bp
  • G to A, chromosome 2 at 169,884,642 bp
  • T to A, chromosome 3 at 52,345,880 bp
  • T to G, chromosome 3 at 87,367,625 bp
  • A to G, chromosome 3 at 97,751,807 bp
  • G to T, chromosome 3 at 116,328,564 bp
  • T to G, chromosome 4 at 63,133,274 bp
  • G to T, chromosome 4 at 123,509,893 bp
  • T to C, chromosome 5 at 24,159,467 bp
  • C to T, chromosome 5 at 52,184,856 bp
  • C to A, chromosome 5 at 115,604,885 bp
  • T to A, chromosome 5 at 127,792,506 bp
  • C to T, chromosome 6 at 24,097,579 bp
  • T to C, chromosome 6 at 67,766,769 bp
  • T to A, chromosome 6 at 123,741,823 bp
  • T to A, chromosome 7 at 4,933,433 bp
  • C to T, chromosome 7 at 8,368,092 bp
  • T to A, chromosome 7 at 13,032,718 bp
  • C to A, chromosome 7 at 16,561,624 bp
  • T to G, chromosome 7 at 81,463,798 bp
  • T to G, chromosome 7 at 96,823,873 bp
  • T to C, chromosome 7 at 127,521,990 bp
  • T to C, chromosome 7 at 143,618,393 bp
  • T to C, chromosome 8 at 45,885,674 bp
  • T to C, chromosome 8 at 70,340,024 bp
  • G to A, chromosome 8 at 79,975,114 bp
  • T to C, chromosome 8 at 119,666,563 bp
  • A to G, chromosome 9 at 4,330,584 bp
  • A to T, chromosome 9 at 13,245,399 bp
  • T to A, chromosome 9 at 38,368,082 bp
  • A to G, chromosome 9 at 79,641,447 bp
  • A to G, chromosome 9 at 95,998,221 bp
  • A to G, chromosome 9 at 103,328,568 bp
  • C to T, chromosome 10 at 7,702,288 bp
  • A to G, chromosome 10 at 50,896,069 bp
  • G to T, chromosome 10 at 76,081,331 bp
  • T to C, chromosome 10 at 88,463,872 bp
  • A to G, chromosome 10 at 89,589,582 bp
  • A to G, chromosome 10 at 117,818,486 bp
  • A to G, chromosome 11 at 46,127,349 bp
  • T to G, chromosome 11 at 58,220,454 bp
  • G to T, chromosome 11 at 60,709,576 bp
  • A to G, chromosome 11 at 106,326,250 bp
  • C to T, chromosome 12 at 21,351,587 bp
  • A to T, chromosome 12 at 75,962,575 bp
  • C to A, chromosome 13 at 19,376,639 bp
  • C to G, chromosome 13 at 44,911,251 bp
  • C to T, chromosome 13 at 59,462,070 bp
  • T to C, chromosome 13 at 77,245,281 bp
  • T to A, chromosome 13 at 80,893,387 bp
  • T to A, chromosome 13 at 81,419,422 bp
  • T to C, chromosome 14 at 68,629,082 bp
  • A to G, chromosome 15 at 76,049,259 bp
  • T to C, chromosome 15 at 85,896,895 bp
  • T to C, chromosome 15 at 98,814,019 bp
  • T to C, chromosome 16 at 34,690,748 bp
  • A to G, chromosome 16 at 96,127,309 bp
  • G to A, chromosome 17 at 8,962,959 bp
  • A to C, chromosome 17 at 25,491,610 bp
  • A to G, chromosome 17 at 46,498,934 bp
  • T to C, chromosome 18 at 30,294,430 bp
  • A to T, chromosome 18 at 80,128,985 bp
  • T to A, chromosome 19 at 30,134,286 bp
  • G to A, chromosome 19 at 34,235,234 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9163 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068975-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.