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Strain Name:
C57BL/6J-MtgxR9333Btlr/Mmmh
Stock Number:
069145-MU
Citation ID:
RRID:MMRRC_069145-MU
Other Names:
R9333 (G1)
Major Collection:

Strain Information

Hcn2
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 2
Synonyms: HAC1, trls
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15166
HGNC: HGNC:4846
Homologene: 31022
Gtf2i
Name: general transcription factor II I
Synonyms: TFII-I, BAP-135, 6030441I21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14886
HGNC: HGNC:4659
Homologene: 7748
Arhgap39
Name: Rho GTPase activating protein 39
Synonyms: D15Wsu169e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223666
Homologene: 27825
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: nonmuscle myosin heavy chain II-B, NMHC-B, SMemb, nonmuscle myosin heavy chain IIB, 9330167F11Rik, 5730504C04Rik, NMHC II-B, Myhn2, Myosin IIB, Myhn-2, myosin IIB, Fltn, Fltn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Tcf7l2
Name: transcription factor 7 like 2, T cell specific, HMG box
Synonyms: TCF4E, TCF4B, mTcf-4E, mTcf-4B, Tcf-4, Tcf4
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21416
Homologene: 7564
Arfgef1
Name: ARF guanine nucleotide exchange factor 1
Synonyms: D730028O18Rik, ARFGEP1, P200, BIG1, D130059B05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 211673
Homologene: 4687
Prmt3
Name: protein arginine N-methyltransferase 3
Synonyms: 2410018A17Rik, 2010005E20Rik, Hrmt1l3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71974
Homologene: 24255
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to A, chromosome 1 at 10,151,812 bp (GRCm38)
  • A to T, chromosome 1 at 135,264,124 bp (GRCm38)
  • T to A, chromosome 2 at 30,397,128 bp (GRCm38)
  • A to T, chromosome 2 at 101,630,407 bp (GRCm38)
  • A to T, chromosome 2 at 121,954,492 bp (GRCm38)
  • G to A, chromosome 2 at 122,101,763 bp (GRCm38)
  • A to G, chromosome 2 at 140,193,593 bp (GRCm38)
  • A to G, chromosome 2 at 177,509,126 bp (GRCm38)
  • T to C, chromosome 3 at 98,741,900 bp (GRCm38)
  • T to C, chromosome 4 at 63,356,291 bp (GRCm38)
  • C to T, chromosome 4 at 72,852,130 bp (GRCm38)
  • G to T, chromosome 4 at 123,860,623 bp (GRCm38)
  • A to G, chromosome 5 at 34,635,083 bp (GRCm38)
  • C to A, chromosome 5 at 64,105,757 bp (GRCm38)
  • T to C, chromosome 5 at 100,812,073 bp (GRCm38)
  • C to T, chromosome 5 at 134,242,986 bp (GRCm38)
  • A to G, chromosome 6 at 71,795,214 bp (GRCm38)
  • T to C, chromosome 6 at 72,337,727 bp (GRCm38)
  • T to C, chromosome 6 at 106,800,023 bp (GRCm38)
  • C to T, chromosome 6 at 124,026,050 bp (GRCm38)
  • A to G, chromosome 6 at 126,975,133 bp (GRCm38)
  • T to A, chromosome 6 at 130,044,117 bp (GRCm38)
  • T to C, chromosome 7 at 4,793,080 bp (GRCm38)
  • T to A, chromosome 7 at 7,289,193 bp (GRCm38)
  • C to T, chromosome 7 at 7,672,368 bp (GRCm38)
  • A to C, chromosome 7 at 29,074,789 bp (GRCm38)
  • T to G, chromosome 7 at 49,806,560 bp (GRCm38)
  • T to C, chromosome 7 at 79,417,846 bp (GRCm38)
  • T to C, chromosome 7 at 92,928,686 bp (GRCm38)
  • T to C, chromosome 7 at 106,752,575 bp (GRCm38)
  • C to A, chromosome 9 at 106,648,217 bp (GRCm38)
  • T to A, chromosome 10 at 79,726,157 bp (GRCm38)
  • T to C, chromosome 10 at 80,524,346 bp (GRCm38)
  • A to C, chromosome 11 at 68,790,154 bp (GRCm38)
  • A to T, chromosome 11 at 72,317,523 bp (GRCm38)
  • G to A, chromosome 12 at 103,345,696 bp (GRCm38)
  • T to C, chromosome 13 at 34,916,893 bp (GRCm38)
  • T to C, chromosome 14 at 102,981,618 bp (GRCm38)
  • A to G, chromosome 15 at 3,969,020 bp (GRCm38)
  • C to T, chromosome 15 at 76,735,125 bp (GRCm38)
  • G to A, chromosome 15 at 89,355,374 bp (GRCm38)
  • T to A, chromosome 17 at 23,743,801 bp (GRCm38)
  • C to A, chromosome 17 at 25,248,702 bp (GRCm38)
  • C to A, chromosome 19 at 55,931,496 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9333 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069145-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.