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Strain Name:
C57BL/6J-MtgxR9577Btlr/Mmmh
Stock Number:
069370-MU
Citation ID:
RRID:MMRRC_069370-MU
Other Names:
R9577 (G1)
Major Collection:

Strain Information

Kif3a
Name: kinesin family member 3A
Synonyms: kinesin-II subunit, Kns3, Kif3, Kifl, N-4 kinesin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16568
HGNC: HGNC:6319
Homologene: 38266
Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Tm9sf4
Name: transmembrane 9 superfamily member 4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99237
Homologene: 21620
Atad5
Name: ATPase family, AAA domain containing 5
Synonyms: LOC237877, C130052G03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237877
Homologene: 32611
Fbxo42
Name: F-box protein 42
Synonyms: 6720460I06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 213499
Homologene: 16234
Arap2
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Synonyms: Centd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 212285
Homologene: 9064
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 36,428,409 bp (GRCm38)
  • C to A, chromosome 1 at 72,681,908 bp (GRCm38)
  • T to C, chromosome 1 at 136,471,400 bp (GRCm38)
  • T to C, chromosome 1 at 139,090,458 bp (GRCm38)
  • T to A, chromosome 1 at 176,831,063 bp (GRCm38)
  • CAAA to CAA, chromosome 1 at 189,256,694 bp (GRCm38)
  • T to A, chromosome 2 at 26,352,332 bp (GRCm38)
  • T to C, chromosome 2 at 30,101,038 bp (GRCm38)
  • C to A, chromosome 2 at 36,812,555 bp (GRCm38)
  • T to C, chromosome 2 at 59,978,687 bp (GRCm38)
  • C to T, chromosome 2 at 113,364,125 bp (GRCm38)
  • T to A, chromosome 2 at 129,016,182 bp (GRCm38)
  • T to C, chromosome 2 at 153,195,374 bp (GRCm38)
  • A to G, chromosome 4 at 8,752,964 bp (GRCm38)
  • T to A, chromosome 4 at 141,180,432 bp (GRCm38)
  • A to G, chromosome 4 at 149,383,774 bp (GRCm38)
  • C to T, chromosome 5 at 20,609,284 bp (GRCm38)
  • A to T, chromosome 5 at 62,611,717 bp (GRCm38)
  • T to A, chromosome 5 at 64,316,356 bp (GRCm38)
  • T to C, chromosome 5 at 87,080,925 bp (GRCm38)
  • A to T, chromosome 5 at 94,383,946 bp (GRCm38)
  • C to A, chromosome 5 at 103,528,809 bp (GRCm38)
  • A to G, chromosome 5 at 143,196,300 bp (GRCm38)
  • T to C, chromosome 5 at 146,168,890 bp (GRCm38)
  • G to T, chromosome 6 at 23,002,203 bp (GRCm38)
  • A to T, chromosome 7 at 18,612,142 bp (GRCm38)
  • A to T, chromosome 7 at 45,305,008 bp (GRCm38)
  • G to T, chromosome 7 at 45,727,015 bp (GRCm38)
  • T to C, chromosome 7 at 120,211,545 bp (GRCm38)
  • T to C, chromosome 7 at 127,934,501 bp (GRCm38)
  • T to C, chromosome 7 at 141,211,077 bp (GRCm38)
  • A to G, chromosome 7 at 143,079,394 bp (GRCm38)
  • A to G, chromosome 8 at 84,256,300 bp (GRCm38)
  • A to G, chromosome 8 at 85,776,169 bp (GRCm38)
  • A to G, chromosome 9 at 39,258,441 bp (GRCm38)
  • G to A, chromosome 9 at 48,326,332 bp (GRCm38)
  • A to G, chromosome 9 at 54,416,380 bp (GRCm38)
  • A to G, chromosome 9 at 87,729,459 bp (GRCm38)
  • C to T, chromosome 9 at 108,463,273 bp (GRCm38)
  • G to A, chromosome 9 at 121,642,927 bp (GRCm38)
  • C to T, chromosome 10 at 21,154,713 bp (GRCm38)
  • A to G, chromosome 10 at 60,311,116 bp (GRCm38)
  • A to G, chromosome 11 at 53,466,785 bp (GRCm38)
  • T to C, chromosome 11 at 53,584,404 bp (GRCm38)
  • C to T, chromosome 11 at 65,976,521 bp (GRCm38)
  • C to T, chromosome 11 at 72,037,427 bp (GRCm38)
  • T to A, chromosome 11 at 80,114,170 bp (GRCm38)
  • T to C, chromosome 11 at 121,624,214 bp (GRCm38)
  • A to G, chromosome 12 at 55,860,091 bp (GRCm38)
  • T to A, chromosome 15 at 63,825,057 bp (GRCm38)
  • T to C, chromosome 15 at 71,965,746 bp (GRCm38)
  • A to T, chromosome 17 at 28,909,408 bp (GRCm38)
  • A to G, chromosome 17 at 34,384,361 bp (GRCm38)
  • G to T, chromosome 18 at 24,475,606 bp (GRCm38)
  • C to T, chromosome 18 at 37,022,073 bp (GRCm38)
  • T to C, chromosome 18 at 38,330,552 bp (GRCm38)
  • C to G, chromosome 18 at 42,395,498 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9577 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069370-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.