Strain Detail Sheet

Strain Name:
MLX flox/flox
Stock Number:
069781-UCD
Citation ID:
RRID:MMRRC_069781-UCD
Other Names:
MLX flox/flox

Strain Information

Gene Details

Mlxtm1.1Rne
Name: MAX-like protein X; targeted mutation 1.1, Robert N Eisenman
Synonyms: Mlx floxed
Type: Allele
Species: Mus musculus
Chromosome: 11
Alteration at locus: Conditional
Mlx
Name: MAX-like protein X
Synonyms: Tcfl4, bHLHd13, BigMax alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Conditional
NCBI: 21428
Homologene: 7969
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A loxP site was inserted upstream of exon 3 and a loxP site and FRT flanked neomycin cassette were inserted downstream of exon 6 via homologous recombination. FLP-mediated recombination removed the neomycin selection cassette, leaving loxP sites flanking exons 3-6.
ES Cell Line
129Sv

Strain Description

Phenotype
Mice exhibit normal embryogenesis and development. However males are infertile (females are fertile). Also recovery from cardiotoxin induced muscle injury is compromised.

Conditional phenotype: Male infertility phenotype described above (male infertility) is entirely conditional upon Cre induced deletion.

Strain Development
-Targeting vector based on PGKneoF2L2DTA (LoxP-FRT-pGKNeobpA-FRT-LoxP) (from Phil Soriano) and lox P sites flanking exons 3-6 of murine gene Mlx (Transcript ENSMUST00000017945.15 Mlx201) (will delete the entire HLH basic region)
-Electroporation of linearized target vector into AK7 ES cells (129Sv) (from Phil Soriano)
-G418 selection, clonal expansion, and screening via Southern for correct targeting into Mlx locus
-Two ES clones injected into blastocysts (C57Bl/6) to produce chimeras
-Mating of male chimera with FLPeR female (129Sv) (from Phil Soriano) to delete the neo cassette confirmed via southern and PCR
-Mating with 129Sv to remove FLpeR to produce pure 129Sv conditional Mlx partial knockout mouse.
Suggested Control Mice
Wild type or heterozygous MLX 129Sv mice.

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Metabolism
  • Reproduction
  • Research Tools

Strain Origin

Donor
Robert Eisenman, Ph.D., Fred Hutchinson Cancer Center.
Primary Reference

Carroll PA, Freie BW, Cheng PF, Kasinathan S, Gu H, Hedrich T, Dowdle JA, Venkataramani V, Ramani V, Wu X, Raftery D, Shendure J, Ayer DE, Muller CH, Eisenman RN. The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis. PLoS Biol. 2021 Oct 20;19(10):e3001085. doi: 10.1371/journal.pbio.3001085. (Medline PMCID: 8528285)

Carroll PA, Diolaiti D, McFerrin L, Gu H, Djukovic D, Du J, Cheng PF, Anderson S, Ulrich M, Hurley JB, Raftery D, Ayer DE, Eisenman RN. Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis. Cancer Cell. 2015 Feb 9;27(2):271-85. doi: 10.1016/j.ccell.2014.11.024. Epub 2015 Jan 29. (Medline PMCID: 4326605)

Hunt LC, Xu B, Finkelstein D, Fan Y, Carroll PA, Cheng PF, Eisenman RN, Demontis F. The glucose-sensing transcription factor MLX promotes myogenesis via myokine signaling. Genes Dev. 2015 Dec 1;29(23):2475-89. doi: 10.1101/gad.267419.115. Epub 2015 Nov 19. (Medline PMCID: 4691951)

https://doi.org/10.1016/j.jcmgh.2022.02.018Wang, Huabo, Lu, Jie, Alencastro, Frances, Roberts, Alexander, Fiedor, Julia, Carroll, Patrick, Eisenman, Robert N., Ranganathan, Sarangarajan, Torbenson, Michael, Duncan, Andrew W., Prochownik, Edward. Coordinated Cross-Talk Between the Myc and Mlx Networks in Liver Regeneration and NeoplasiaCellular and Molecular Gastroenterology and Hepatology. 2022

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .

Appearance

Coat Color
Agouti
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months 10 to 12 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Danielle Aaker.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Danielle Aaker

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
069781-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.