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Strain Name:
C57BL/6J-MtgxR9742Btlr/Mmmh
Stock Number:
069988-MU
Citation ID:
RRID:MMRRC_069988-MU
Other Names:
R9742 (G1)
Major Collection:

Strain Information

Ldb1
Name: LIM domain binding 1
Synonyms: CLIM2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16825
HGNC: HGNC:6532
Homologene: 2891
Ak2
Name: adenylate kinase 2
Synonyms: Ak-2, D4Ertd220e
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11637
HGNC: HGNC:362
Homologene: 1227
Adgrl2
Name: adhesion G protein-coupled receptor L2
Synonyms: Lphh1, Lec1, Lphn2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99633
Homologene: 22712
Fndc3a
Name: fibronectin type III domain containing 3A
Synonyms: 1700094E19Rik, F730017H24Rik, D14Ertd453e, Fndc3, sys
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319448
Homologene: 8952
Zzef1
Name: zinc finger, ZZ-type with EF hand domain 1
Synonyms: 8430405D05Rik, C130099L13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 195018
Homologene: 9027
Brat1
Name: BRCA1-associated ATM activator 1
Synonyms: Baat1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231841
Homologene: 16776
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to G, chromosome 1 at 21,369,721 bp (GRCm38)
  • T to C, chromosome 1 at 74,904,681 bp (GRCm38)
  • A to G, chromosome 1 at 133,094,749 bp (GRCm38)
  • T to C, chromosome 2 at 37,625,256 bp (GRCm38)
  • T to C, chromosome 2 at 87,252,032 bp (GRCm38)
  • T to C, chromosome 3 at 30,598,285 bp (GRCm38)
  • A to G, chromosome 3 at 148,836,350 bp (GRCm38)
  • T to A, chromosome 4 at 129,008,168 bp (GRCm38)
  • T to A, chromosome 4 at 149,468,881 bp (GRCm38)
  • C to T, chromosome 4 at 154,998,455 bp (GRCm38)
  • A to G, chromosome 5 at 137,138,279 bp (GRCm38)
  • A to G, chromosome 5 at 140,718,157 bp (GRCm38)
  • T to A, chromosome 6 at 120,481,505 bp (GRCm38)
  • CTCTTCTTCTTCTTCTTC to CTCTTCTTCTTCTTC, chromosome 7 at 30,958,067 bp (GRCm38)
  • T to G, chromosome 7 at 79,099,367 bp (GRCm38)
  • A to G, chromosome 7 at 99,914,453 bp (GRCm38)
  • C to T, chromosome 7 at 139,226,313 bp (GRCm38)
  • G to A, chromosome 8 at 13,390,097 bp (GRCm38)
  • A to G, chromosome 8 at 28,666,764 bp (GRCm38)
  • A to T, chromosome 8 at 31,094,170 bp (GRCm38)
  • A to G, chromosome 9 at 57,545,323 bp (GRCm38)
  • C to A, chromosome 10 at 29,148,398 bp (GRCm38)
  • G to A, chromosome 10 at 120,038,664 bp (GRCm38)
  • A to G, chromosome 10 at 128,461,353 bp (GRCm38)
  • G to A, chromosome 11 at 72,858,353 bp (GRCm38)
  • C to A, chromosome 11 at 94,573,137 bp (GRCm38)
  • A to T, chromosome 12 at 3,686,740 bp (GRCm38)
  • A to G, chromosome 12 at 59,204,896 bp (GRCm38)
  • T to C, chromosome 13 at 93,095,427 bp (GRCm38)
  • G to A, chromosome 14 at 33,088,030 bp (GRCm38)
  • T to A, chromosome 14 at 54,956,599 bp (GRCm38)
  • C to A, chromosome 14 at 72,540,253 bp (GRCm38)
  • A to G, chromosome 15 at 57,823,410 bp (GRCm38)
  • G to A, chromosome 15 at 81,016,979 bp (GRCm38)
  • T to A, chromosome 16 at 45,580,253 bp (GRCm38)
  • T to C, chromosome 16 at 60,205,702 bp (GRCm38)
  • A to G, chromosome 17 at 36,889,010 bp (GRCm38)
  • T to C, chromosome 17 at 55,611,009 bp (GRCm38)
  • T to A, chromosome 18 at 77,980,955 bp (GRCm38)
  • A to T, chromosome 19 at 5,729,302 bp (GRCm38)
  • T to C, chromosome 19 at 7,710,916 bp (GRCm38)
  • T to C, chromosome 19 at 13,191,405 bp (GRCm38)
  • G to T, chromosome 19 at 18,823,402 bp (GRCm38)
  • A to G, chromosome 19 at 46,035,419 bp (GRCm38)
  • A to G, chromosome 19 at 46,331,288 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9742 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069988-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.