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Strain Information

Name: dipeptidylpeptidase 4; endonuclease-mediated mutation 1, Mouse Biology Program, UC Davis
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: CRISPR
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; endonuclease-mediated mutation 1, Mouse Biology Program
Type: Allele
Species: Multi-species
Chromosome: X
Alteration at locus: CRISPR
Name: angiotensin converting enzyme 2
Synonyms: 2010305L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: CRISPR
NCBI: 70008
Homologene: 41448
Name: dipeptidylpeptidase 4
Synonyms: Cd26, Dpp-4, THAM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: CRISPR
NCBI: 13482
Homologene: 3279
Genetic Alterations
The mouse gene was deleted and the human gene simultaneously inserted via CRISPR targeting for each, Ace2 and Dpp4, in separate targeting events.
ES Cell Line
JM8A3 derived from C57BL/6N
Strain Development
CRISPR RNP was used to assist with homologous recombination using dsDNA repair template in JM8A3 ES cells derived from C57BL/6N for generation of the Ace2 deletion and ACE2 insertion model . Following blastocyst injection, the chimeras were mated with C57BL/6NCrl to create germline heterozygotes. Long-range PCR products were fully sequenced; potential off-targets were absent. CRISPR/RNP was used to assist with homologous recombination using a dsDNA repair template directly in zygotes for the Dpp4 deletion and DPP4 insertion model. The founder was backcrossed with C57BL/6NCrl to create germline heterozygous animals. Long-range PCR products were sequenced in-full to confirm the absence of potential off-targets. One additional backcross to C57BL/6NCrl. The two targeted lines were then intercrossed to derive mice with both mutations. Mice are homozygous (females) or hemizygous (males) for humanized ACE2, and homozygous for humanized DPP4.
Suggested Control Mice
Sibling wild types or C57BL/6N
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact Older strains may not have this information.
  • Immunology and Inflammation
  • Models for Human Disease
  • Virology
Kent Lloyd, D.V.M., University of California, Davis.
Primary Reference
Not ready to publish

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact
Coat Color
Black or Agouti
MMRRC Breeding System
Random intra-strain mating
Overall Breeding Performance
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes Ace2 is X-linked, males are hemizygous for the ACE2 mutation
Homozygotes are fertile: Yes Yes Ace2 is X-linked, males are hemizygous for the ACE2 mutation
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

The availability level for this product has not been determined.

A license from third party patent owner(s) may be required for for-profit entities to use mouse models derived from CRISPR-Cas9 technologies and it is the Users sole responsibility to determine whether such a license is necessary.

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