Strain Detail Sheet
Strain Name:
C57BL/6N-Ace2em1(ACE2)MbpDpp4em1(DPP4)Mbp/Mmucd
Stock Number:
071288-UCD
Citation ID:
RRID:MMRRC_071288-UCD
Other Names:
830404
Major Collection:
Dpp4em1(DPP4)Mbp
Name: dipeptidylpeptidase 4; endonuclease-mediated mutation 1, Mouse Biology Program, UC Davis
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: CRISPR
MGI: MGI:6856706
Ace2em1(ACE2)Mbp
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; endonuclease-mediated mutation 1, Mouse Biology Program
Type: Allele
Species: Multi-species
Chromosome: X
Alteration at locus: CRISPR
Ace2
Name: angiotensin converting enzyme 2
Synonyms: 2010305L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: CRISPR
MGI: MGI:1917258
NCBI: 70008
Ensembl: ENSMUSG00000015405
VEGA: OTTMUSG00000019518
HGNC: HGNC:13557
Homologene: 41448
CCDS: CCDS30518.1
UCSC: ChrX:162922338-162971414
Dpp4
Name: dipeptidylpeptidase 4
Synonyms: THAM, Dpp-4, Cd26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: CRISPR
MGI: MGI:94919
NCBI: 13482
Ensembl: ENSMUSG00000035000
VEGA: OTTMUSG00000012759
HGNC: HGNC:3009
Homologene: 3279
CCDS: CCDS16065.1
UCSC: Chr2:62160417-62242635
Genetic Alterations
The mouse gene was deleted and the human gene simultaneously inserted via CRISPR targeting for each, Ace2 and Dpp4, in separate targeting events.
ES Cell Line
JM8A3 derived from C57BL/6N
Phenotype
Unknown
Strain Development
CRISPR RNP was used to assist with homologous recombination using dsDNA repair template in JM8A3 ES cells derived from C57BL/6N for generation of the Ace2 deletion and ACE2 insertion model . Following blastocyst injection, the chimeras were mated with C57BL/6NCrl to create germline heterozygotes. Long-range PCR products were fully sequenced; potential off-targets were absent. CRISPR/RNP was used to assist with homologous recombination using a dsDNA repair template directly in zygotes for the Dpp4 deletion and DPP4 insertion model. The founder was backcrossed with C57BL/6NCrl to create germline heterozygous animals. Long-range PCR products were sequenced in-full to confirm the absence of potential off-targets. One additional backcross to C57BL/6NCrl. The two targeted lines were then intercrossed to derive mice with both mutations. Mice are homozygous (females) or hemizygous (males) for humanized ACE2, and homozygous for humanized DPP4.
Suggested Control Mice
Sibling wild types or C57BL/6N
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
- Immunology and Inflammation
- Models for Human Disease
- Virology
Donor
Kent Lloyd, D.V.M., University of California, Davis.
Primary Reference
Not ready to publish
Colony Surveillance Program and Current Health Reports
For more information about this colony's health status contact mmrrc@ucdavis.eduCoat Color
Black or Agouti
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Generation
N/A
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
| Viability and Fertility: | Female | Male | Comments |
|---|---|---|---|
| Homozygotes are viable: | Yes | Yes | Ace2 is X-linked, males are hemizygous for the ACE2 mutation |
| Homozygotes are fertile: | Yes | Yes | Ace2 is X-linked, males are hemizygous for the ACE2 mutation |
| Hetero/Hemizygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | Undetermined | Undetermined |
Bred to Homozygosity
Yes
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9
The availability level for this product has not been determined.
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