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Strain Name:
FVB/NJ-Tg(HHV8)197Dtmr/Mmnc
Stock Number:
071752-UNC
Citation ID:
RRID:MMRRC_071752-UNC
Other Names:
FVB/NJ-Tg(HHV8)197Dtmr/Mmnc

Strain Information

Genetic Alterations

This whole-virus (Kaposi sarcoma Herpesvirus (KSHV) transgenic line harbors 170,000 bp of the Human Herpesvirus-8 (HHV-8), with 12 of its viral microRNAs deleted. All KSHV genes are controlled by their original viral promoters.

Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not applicable
    Phenotype

    These mice develop angiosarcoma or Kaposi Sarcoma (KS) between 3 and 12 months. KS is the most common cancer in persons living with HIV. It is caused by KS-associated herpesvirus (KSHV). Pronuclear injection of the 170,000 bp viral genome induced early-onset, aggressive angiosarcoma in transgenic mice. The tumors were histopathologically indistinguishable from human KS. The KSHV-induced tumors were more aggressive than Ha-ras-induced angiosarcomas. Overall survival could be increased by prophylactic ganciclovir. Thus, whole virus KSHV-transgenic mice (BAC16_ΔmiR-all) represent an accurate model for KS and open the door for the genetic dissection of KS pathogenesis and evaluation of therapies, including vaccines.

    Strain Development
    The transgenic construct 'HHV8' was injected into FVB/N zygotes, and multiple founders were identified. Founder #197 was selected for further breeding and expansion. This male founder was mated to FVB females, and progeny were screened for the presence of the transgene. The colony was then backcrossed to FVB/NJ mice (JAX Strain IMSR_JAX:001800). After the backcrossing was complete, mice were then sibling mated for at least 5 generations to date (F5+).
    Suggested Control Mice
    FVB/NJ
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Cardiovascular
    • Virology
    Donor
    Dirk Dittmer, The University of North Carolina at Chapel Hill.
    Primary Reference

    accepted in Cell Host & Microbe pending MMRRC number

    Colony and Husbandry Information

    Coat Color
    White
    Eye
    Pink
    MMRRC Breeding System
    Backcross
    Generation
    F5+
    Overall Breeding Performance
    Poor
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Undetermined Undetermined
    Homozygotes are fertile: Undetermined Undetermined
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Average litter size
    4 to 6
    Recommended wean age
    4 Weeks
    Average Pups Weaned
    Less than 4

    Order Request Information

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@med.unc.edu for more details.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    071752-UNC-HET-F
    071752-UNC-HET-M
    071752-UNC-WT-F
    071752-UNC-WT-M
    Heterozygous / Hemizygous Female
    Heterozygous / Hemizygous Male
    Wild Type Female
    Wild Type Male
    $311.00 / $516.00
    Non-Profit / For-Profit
    Per Mouse The mmrrc@med.unc.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
    071752-UNC-SPERM Cryo-preserved spermatozoa $520.00 / $863.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.