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Strain Name:
CD-1 Brd4em1GumsuNutm1em1Gumsu/Mmmh
Stock Number:
071753-MU
Citation ID:
RRID:MMRRC_071753-MU
Other Names:
Nut carcinoma translocator

Strain Information

Brd4
Name: bromodomain containing 4
Synonyms: MCAP, HUNK1, WI-11513
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 57261
Homologene: 136213
Nutm1
Name: NUT midline carcinoma, family member 1
Synonyms: Nut, BC125332
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 213765
Homologene: 18185
Genetic Alterations
A T2A-Luc2TdTomato reporter cassette is inserted via CRISPR, replacing the endogenous stop codon of the Nutm1 gene. A loxp site is inserted into intron 1 of the Nutm1 gene and a loxp site is also inserted into intron 12 of the Brd4 gene via CRISPR. The Brd4loxp and Nutm1loxp allele strains were bred to generate double homozygous strains to form the Nut carcinoma translocator mouse line.
ES Cell Line
Not applicable
Phenotype

Conditional phenotype: Crossing this strain with a Cre driver line induces a chromosome translocation that forms the Brd4-Nutm1 fusion gene. This drives aggressive cancer, recapitulating many phenotypes of human Nut carcinoma.

MeSH Terms
  • Animals
  • Mice
  • Bromodomain Containing Proteins
  • Carcinoma/genetics
  • Carcinoma/metabolism
  • Cell Cycle Proteins/genetics
  • Cell Cycle Proteins/metabolism
  • Disease Models, Animal
  • Neoplasm Proteins/genetics
  • Neoplasm Proteins/metabolism
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism
  • Oncogene Proteins, Fusion/genetics
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Translocation, Genetic/genetics
Strain Development
CRISPR guide(s), Cas9-mSA mRNA, and biotinylated CPR donors were microinjected into CD-1 2-cell stage embryos. Injected embryos were implanted into pseudopregnant females to generate founder pups. Founders were mated to CD-1 breeders and derived N1 progeny were identified via PCR. N1 progeny were mated to CD-1 breeders for two generations to generate N3 mice. Heterozygous N3s were bred together to establish a homozygous colony of the Nutm1-T2A-Luc2TdTomato (Nutm1loxp) mouse line. A loxp site was inserted into intron 1 of the Nutm1loxp gene by microinjecting Nutm1loxp homozygous two-cell embryos.

The Brd4 mutant mouse line was generated in a similar manner. Injected embryos were implanted into pseudopregnant females to generate founder mice which then followed the breeding scheme described above to generate N3 mice. The Brd4loxp and Nutm1loxp strains were bred to double homozygous strains to generate the Nut carcinoma translocator mouse line.
Suggested Control Mice
when generating tumors, cre negative littermates should be used as controls
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Models for Human Disease
Donor
Bin Gu, Ph.D., Michigan State University.
Primary Reference

Zheng D, Elnegiry AA, Luo C, Bendahou MA, Xie L, Bell D, Takahashi Y, Hanna E, Mias GI, Tsoi MF, Gu B. Brd4::Nutm1 fusion gene initiates NUT carcinoma in vivo. Life Sci Alliance. 2024 May 9;7(7):e202402602. doi: 10.26508/lsa.202402602. (Medline PMID: 38724194)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
White
Eye
Pink
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
Back-cross to Crl:CD1(ICR) or inbred
Generation
N/A
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 10 to 12 months
Average litter size
10 to 14
Recommended wean age
3 Weeks
Average Pups Weaned
10 to 14

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
071753-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
071753-MU-RESUS Litter recovered from cryo-archive $2,697.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.