Strain Name:
C57BL/6J-Inpp5dm1Btlr/Mmucd
Stock Number:
030820-UCD
Citation ID:
RRID:MMRRC_030820-UCD
Other Names:
styx, C57BL/6J-Inpp5dm1Btlr/Mmcd
Major Collection:

Strain Information

Inpp5dm1Btlr
Name: inositol polyphosphate-5-phosphatase D; mutation 1, Bruce Beutler
Synonyms: styx
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Chemically Induced
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Genetic Alterations
The styx mutation corresponds to a T to A transversion in the donor splice site of intron 5 (GTAAC->GAAAC) of the Inpp5d gene (position 49406 in the Genbank genomic region NC_000067 for linear genomic DNA sequence of Inpp5d).
Genotype Determination
Phenotype
Homozygous: Homozygous mice have progressive wasting disease and exhibit a failure to thrive, with some mice, especially females, dying spontaneously. Thymic hypertrophy, splenomegaly and aberrant B cell development have been observed in these animals. Furthermore, infiltration of the lungs with myeloid cells was also noted. The phenotype of styx mice is highly similar to allelic Inpp5d (Ship-1) knockout mice.

Heterozygous: Wild-type.
Mammalian Phenotype Terms
Allelic Composition: Inpp5dm1Btlr/Inpp5dm1Btlr (Genetic Background: C57BL/6J-Inpp5dm1Btlr )

Strain Development
The styx mutation was generated on a C57BL/6J background by ENU mutagenesis. The strain has been maintained on this background.
Suggested Control Mice
  • Wild-type littermates
  • C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Immunology and inflammation
  • Models for human disease
Donor
Bruce Beutler, M.D., The Scripps Research Institute, La Jolla, CA
Primary Reference
Krebs P, Smart N, Beutler B. Record for styx, updated February 11, 2010. MUTAGENETIX (TM), B. Beutler and colleagues, Department of Genetics, The Scripps Research Institute, La Jolla, CA..

Colony and Husbandry Information

50% of homozygous mice die within the first 100 days of being born. Therefore, homozygous mice are difficult to breed due to the progressive wasting disease.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Other
Homozygous mice exhibit wasting disease and are obviously lean
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
  • Heterozygous female x Heterozygous male
  • Homozygous female x Homozygous male
Overall Breeding Performance
Good
Average litter size
4-6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030820-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.