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Homozygous: Complete embryonic lethality
Heterozygous: T-independent IgM responses were normal in Worker mice (T-independent B cell response screen). Mice with the Worker mutation displayed moderate CD4+ and CD8+ T cell deficiency and suboptimal T-dependent IgG responses to rSFV-encoded β-galactosidase in the T-dependent humoral response screen. Belly spotting: Expressivity of the pigmentation phenotype varies, ranging from a few white hairs to large patches covering the abdomen. In a backcross of Worker heterozygotes to Wild-type mice, heterozygotes are born at a frequency far beneath the expected Mendelian ratio. Worker mice had significant changes in thymic cell subsets (i.e. DN and SP thymocytes were increased; DP thymocytes were decreased). The Worker mice have fewer splenic CD4+ and CD8+ T cells and they express high levels of surface CD44. To test for extrafollicular antibody responses, the Worker mice were injected with NP-CGG. Seven days post-immunization the mice were assayed and found to have undetectable levels of NP-specific IgM and low levels of IgG1. At day 14 post-immunization, the Worker IgG1 and IgM responses were normal; at 28 days post-immunization, most of the NP-specific IgG1 was high affinity, indicating that affinity maturation occurs in Worker. Few individuals survive to term and to weaning.
Arnold CN, Pirie E, Dosenovic P, McInerney GM, Xia Y, Wang N, Li X, Siggs OM, Karlsson Hedestam GB, Beutler B. A forward genetic screen reveals roles for Nfkbid, Zeb1, and Ruvbl2 in humoral immunity. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12286-93. Epub 2012 Jul 3. (Medline PMID: 22761313).
Arnold CN, Pirie D, Moresco EY, Murray A, Beutler B. Record for Worker. MUTAGENETIXtm, B. Beutler and colleagues, The Scripps Research Institute, La Jolla, CA.
Colony Surveillance Program and Current Health Reports
Wild-type female x Heterozygous male from the colony or reciprocal mating
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.