Strain Name:
C57BL/6J-Efnb3m1Btlr/Mmmh
Stock Number:
036909-MU
Citation ID:
RRID:MMRRC_036909-MU
Other Names:
turtle
Major Collection:

Strain Information

Efnb3m1Btlr
Name: ephrin B3; mutation 1, Bruce Beutler
Synonyms: turtle
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
Efnb3
Name: ephrin B3
Synonyms: LERK-8, NLERK-2, Epl8, EFL-6, ELF-3, Elk-L3
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 13643
HGNC: HGNC:3228
Homologene: 1076
Genetic Alterations
Whole genome SOLiD sequencing identified 19 mutations in a ENU-induced stock mouse. Three G3 mice with the turtle mutation were genotyped at all 19 mutation sites and three mutations on chromosome 11 were homozygous in all three mice. Capillary sequencing of the mutated genes identified a T to C transition at base pair 69370785 (v37) on Chromosome 11 in the GenBank genomic region NC_000077 encoding Efnb3. The mutation corresponds to residue 332 in exon 2 of 5 total exons. The mutation results in a leucine (L) to proline (P) substitution at residue 111.
Genotype Determination
Phenotype

Homozygous: The turtle mouse was visually identified among G3 mice homozygous for mutations induced by N-ethyl-N-nitrosourea (ENU) by a defect in their ability to right themselves when placed on their back and an inability to stay standing; turtle displays normal behavior (e.g., feeding and grooming). Older animals exhibit a "hopping" or mirror movements when walking and running.

Heterozygous: Not evaluated

Mammalian Phenotype Terms
Allelic Composition: Efnb3m1Btlr/Efnb3m1Btlr (Genetic Background: involves: C57BL/6J )

  • behavior/neurological
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Immunology and Inflammation
  • Neurobiology
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
Purrington T, Murray A, Beutler B. Record for turtle. MUTAGENETIXtm, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)

Wild-type female x Heterozygous male from the colony or reciprocal mating

Homozygous female x Homozygous male from the colony

Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4-6
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036909-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.