Strain Name:
C57BL/6J-MtgxP0023Btlr/Mmmh
Stock Number:
038276-MU
Citation ID:
RRID:MMRRC_038276-MU
Other Names:
P0023 (G1), C57BL/6J-MtgxP0023Btlr
Major Collection:

Strain Information

Dcaf1
Name: DDB1 and CUL4 associated factor 1
Synonyms: Vprbp, B930007L02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 321006
Homologene: 8805
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: Lba, D3Ertd775e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: E130014J05Rik, Ayu17-449
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214133
Homologene: 49498
Rsf1
Name: remodeling and spacing factor 1
Synonyms: Hbxap, p325, XAP8, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233532
Homologene: 41142
Gapvd1
Name: GTPase activating protein and VPS9 domains 1
Synonyms: 4432404J10Rik, 2010005B09Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66691
Homologene: 32637
Clptm1l
Name: CLPTM1-like
Synonyms: C130052I12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218335
VEGA: 13
Homologene: 12767
Fanci
Name: Fanconi anemia, complementation group I
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 208836
Homologene: 49530
Lrpprc
Name: leucine-rich PPR-motif containing
Synonyms: Lrp130, 3110001K13Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72416
Homologene: 32695
Actr3
Name: ARP3 actin-related protein 3
Synonyms: Arp3, 1200003A09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74117
HGNC: HGNC:170
Homologene: 68483
Efhc1
Name: EF-hand domain (C-terminal) containing 1
Synonyms: mRib72-1, myoclonin1, 1700029F22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71877
Homologene: 10003
Prkag3
Name: protein kinase, AMP-activated, gamma 3 non-catalytic subunit
Synonyms: AMPKg3S, AMPKg3L, AMPKg3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241113
HGNC: HGNC:9387
Homologene: 23006
Ttpal
Name: tocopherol (alpha) transfer protein-like
Synonyms: 3110080A02Rik, 5830472M02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76080
Homologene: 41562
Pld1
Name: phospholipase D1
Synonyms: Pld1b, Pld1a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18805
HGNC: HGNC:9067
Homologene: 116234
Chp2
Name: calcineurin-like EF hand protein 2
Synonyms: Cbhp2, 2010110P09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70261
Homologene: 11131
Or8b50
Name: olfactory receptor family 8 subfamily B member 50
Synonyms: Olfr914, GA_x6K02T2PVTD-32308823-32309773, MOR165-7
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258782
VEGA: 9
Homologene: 115511
Cited2
Name: Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
Synonyms: p35srj, Msg2, Mrg1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17684
VEGA: 10
HGNC: HGNC:1987
Homologene: 4433
Gm11992
Name: predicted gene 11992
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 626870
Homologene: 77951
Cmya5
Name: cardiomyopathy associated 5
Synonyms: 2310076E21Rik, Myospryn, 2310076E16Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76469
VEGA: 13
Homologene: 137367
Fyb1
Name: FYN binding protein 1
Synonyms: FYB-120/130, ADAP, B630013F22Rik, Fyb
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 23880
HGNC: HGNC:4036
Homologene: 22664
Piezo2
Name: piezo-type mechanosensitive ion channel component 2
Synonyms: Piezo2, Fam38b, 9030411M15Rik, Fam38b2, 9430028L06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 667742
Homologene: 49695
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 20,955,527 bp
  • A to G, chromosome 1 at 74,740,739 bp
  • A to T, chromosome 1 at 125,395,066 bp
  • A to G, chromosome 2 at 34,706,688 bp
  • T to C, chromosome 2 at 163,611,809 bp
  • T to C, chromosome 3 at 28,048,125 bp
  • A to G, chromosome 3 at 86,417,935 bp
  • C to A, chromosome 3 at 133,486,893 bp
  • A to C, chromosome 7 at 79,402,300 bp
  • T to C, chromosome 7 at 97,662,271 bp
  • A to G, chromosome 7 at 122,222,138 bp
  • A to G, chromosome 9 at 38,606,645 bp
  • T to A, chromosome 9 at 106,860,451 bp
  • A to G, chromosome 10 at 17,724,715 bp
  • T to C, chromosome 11 at 9,052,846 bp
  • G to T, chromosome 13 at 73,604,952 bp
  • C to T, chromosome 13 at 93,089,346 bp
  • A to T, chromosome 15 at 6,651,854 bp
  • G to A, chromosome 17 at 84,726,338 bp
  • A to G, chromosome 18 at 63,386,200 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for P0023 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038276-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.