Strain Name:
C57BL/6J-MtgxP0045Btlr/Mmmh
Stock Number:
038292-MU
Citation ID:
RRID:MMRRC_038292-MU
Other Names:
P0045 (G1), C57BL/6J-MtgxP0045Btlr
Major Collection:

Strain Information

Pcm1
Name: pericentriolar material 1
Synonyms: C030044G17Rik, 9430077F19Rik, 2600002H09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18536
HGNC: HGNC:8727
Homologene: 4518
Ap2b1
Name: adaptor-related protein complex 2, beta 1 subunit
Synonyms: 1300012O03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71770
HGNC: HGNC:563
Homologene: 137384
Yeats2
Name: YEATS domain containing 2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208146
VEGA: 16
Homologene: 9967
Ddx42
Name: DEAD box helicase 42
Synonyms: B430002H05Rik, 1810047H21Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 42, SF3b125
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72047
Homologene: 49137
Ubac2
Name: ubiquitin associated domain containing 2
Synonyms: Phgdhl1, 1190008A14Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68889
VEGA: 14
Homologene: 18642
Immt
Name: inner membrane protein, mitochondrial
Synonyms: 1700082C19Rik, HMP, D830041H16Rik, mitofilin, P87/89, P87, Micos60, P89
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 76614
HGNC: HGNC:6047
Homologene: 38234
Tpm3
Name: tropomyosin 3, gamma
Synonyms: Trop-5, gamma-TM, Tpm-5, Tm5NM, hTMnm, hTM30nm, Tpm5, skalphaTM.2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 59069
Homologene: 81889
Litaf
Name: LPS-induced TN factor
Synonyms: 3222402J11Rik, N4WBP3, Nedd4 WW domain-binding protein 3, TBX1 protein
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 56722
Homologene: 37974
Med12l
Name: mediator complex subunit 12-like
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329650
Homologene: 43143
Tnfsf8
Name: tumor necrosis factor (ligand) superfamily, member 8
Synonyms: Cd30L, CD30LG, CD153
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21949
Homologene: 950
Pramel1
Name: PRAME like 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 83491
Homologene: 87078
Kcp
Name: kielin/chordin-like protein
Synonyms: Crim2, KCP, LOC333088
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 333088
Homologene: 87817
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: b2b1203Clo, Dnahc11, avc4, b2b1289Clo, b2b1279Clo, lrd, b2b598Clo, b2b1727Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Efcab6
Name: EF-hand calcium binding domain 6
Synonyms: 4932408N08Rik, 4931407K02Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 77627
Homologene: 11259
Prss32
Name: serine protease 32
Synonyms: 2010001P08Rik, tryptase-5, mT5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 69814
Homologene: 84859
Chsy3
Name: chondroitin sulfate synthase 3
Synonyms: 4833446K15Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 78923
VEGA: 18
Homologene: 28624
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 3 at 59,091,535 bp
  • T to C, chromosome 3 at 90,091,093 bp
  • A to G, chromosome 4 at 63,851,167 bp
  • C to T, chromosome 4 at 143,398,522 bp
  • A to G, chromosome 6 at 29,498,348 bp
  • T to G, chromosome 6 at 71,868,617 bp
  • T to A, chromosome 8 at 41,288,097 bp
  • A to G, chromosome 9 at 79,647,611 bp
  • T to A, chromosome 11 at 83,368,026 bp
  • T to A, chromosome 11 at 106,231,272 bp
  • A to G, chromosome 12 at 118,030,327 bp
  • T to C, chromosome 14 at 121,973,596 bp
  • T to C, chromosome 15 at 83,918,199 bp
  • C to T, chromosome 16 at 10,963,365 bp
  • A to G, chromosome 16 at 20,156,945 bp
  • A to G, chromosome 17 at 23,859,320 bp
  • C to A, chromosome 18 at 59,409,006 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for P0045 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038292-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.