Strain Name:
C57BL/6J-MtgxR0519Btlr/Mmmh
Stock Number:
038712-MU
Citation ID:
RRID:MMRRC_038712-MU
Other Names:
R0519 (G1), C57BL/6J-MtgxR0519Btlr
Major Collection:

Strain Information

Hapln1
Name: hyaluronan and proteoglycan link protein 1
Synonyms: CLP, link protein, LP-1, cartilage linking protein 1, Crtl1l, Crtl1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12950
VEGA: 13
HGNC: HGNC:2380
Homologene: 1420
Colgalt2
Name: collagen beta(1-O)galactosyltransferase 2
Synonyms: Glt25d2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269132
Homologene: 22865
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Aclp7, trabeculin alpha, Acf7
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Clasrp
Name: CLK4-associating serine/arginine rich protein
Synonyms: SWAP2, Sfrs16, Srsf16
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 53609
Homologene: 134306
Rad54l2
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: Srisnf2l, G630026H09Rik, Arip4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81000
Homologene: 56698
Hmgn3
Name: high mobility group nucleosomal binding domain 3
Synonyms: TRIP7, HMGN3b, 6330514M13Rik, HMGN3a, 1110002A15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 94353
2810004N23Rik
Name: RIKEN cDNA 2810004N23 gene
Synonyms: GtAyu21-55, Ayu21-55, Gt(Ayu21)55Imeg
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66523
Homologene: 11982
Dip2c
Name: disco interacting protein 2 homolog C
Synonyms: 2900024P20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 208440
Homologene: 40996
Ube2o
Name: ubiquitin-conjugating enzyme E2O
Synonyms: B230113M03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217342
Homologene: 11113
Ercc6
Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
Synonyms: C130058G22Rik, CSB, CS group B correcting gene
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319955
VEGA: 14
HGNC: HGNC:3438
Homologene: 133552
Ubr7
Name: ubiquitin protein ligase E3 component n-recognin 7 (putative)
Synonyms: 5730410I19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66622
VEGA: 12
Homologene: 11998
Mcm10
Name: minichromosome maintenance 10 replication initiation factor
Synonyms: 2410041F14Rik, C330019M07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70024
Homologene: 41275
Soat1
Name: sterol O-acyltransferase 1
Synonyms: hid, Acact, 8430426K15Rik, ACAT-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20652
Homologene: 2333
Slc39a4
Name: solute carrier family 39 (zinc transporter), member 4
Synonyms: 1600025H15Rik, AWMS2, zip4
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 72027
VEGA: 15
Homologene: 15638
Pus10
Name: pseudouridylate synthase 10
Synonyms: Ccdc139, 2810013G11Rik, 4933435A13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74467
Homologene: 12566
Frem1
Name: Fras1 related extracellular matrix protein 1
Synonyms: heb, eyem02Jus, eyes2, QBRICK, crf11
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329872
Homologene: 27049
Jcad
Name: junctional cadherin 5 associated
Synonyms: 9430020K01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240185
VEGA: 18
Homologene: 19370
Tjp1
Name: tight junction protein 1
Synonyms: ZO-1, ZO1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21872
Homologene: 2445
Hyls1
Name: HYLS1, centriolar and ciliogenesis associated
Synonyms: hydrolethalus syndrome 1, 3010015K02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76832
Homologene: 82283
Dpy19l2
Name: dpy-19 like 2
Synonyms: 4932443J21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320752
VEGA: 9
Homologene: 77569
Glb1
Name: galactosidase, beta 1
Synonyms: Bge, Bgt, Bgl-t, C130097A14Rik, Bgl-e, Bgl-s, Bgl, Bgs
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12091
VEGA: 9
HGNC: HGNC:4298
Homologene: 47922
Lifr
Name: LIF receptor alpha
Synonyms: A230075M04Rik, soluble differentiation-stimulating factor receptor
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16880
VEGA: 15
HGNC: HGNC:6597
Homologene: 1735
Mxra7
Name: matrix-remodelling associated 7
Synonyms: E130302J09Rik, 1810057P16Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67622
HGNC: HGNC:7541
Homologene: 137378
Asxl3
Name: ASXL transcriptional regulator 3
Synonyms: D430002O22Rik, LOC381127, D930044O18Rik, C230079D11Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 211961
Homologene: 19371
Rad54b
Name: RAD54 homolog B (S. cerevisiae)
Synonyms: E130016E03Rik, E130016E03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 623474
Homologene: 8240
Zp2
Name: zona pellucida glycoprotein 2
Synonyms: Zp-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22787
Homologene: 48194
Gcgr
Name: glucagon receptor
Synonyms: GR
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14527
HGNC: HGNC:4192
Homologene: 131
Ackr4
Name: atypical chemokine receptor 4
Synonyms: VSHK1, A630091E18Rik, CCR11, Ccrl1, CCBP2, CCX-CKR, PPR1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 252837
HGNC: HGNC:1611
Homologene: 49474
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Ptprq
Name: protein tyrosine phosphatase receptor type Q
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237523
HGNC: HGNC:9679
Homologene: 83557
Tgm5
Name: transglutaminase 5
Synonyms: TGx, 2310007C07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74176
Homologene: 20899
Tecta
Name: tectorin alpha
Synonyms: Tctna, [a]-tectorin
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21683
Homologene: 3955
Mapk4
Name: mitogen-activated protein kinase 4
Synonyms: Erk3-related, p63Mapk, A330097D03Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225724
HGNC: HGNC:6878
Homologene: 2058
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
Zbtb38
Name: zinc finger and BTB domain containing 38
Synonyms: A930014K01Rik, Zenon homolog, CIBZ
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245007
Homologene: 19529
Slc2a2
Name: solute carrier family 2 (facilitated glucose transporter), member 2
Synonyms: Glut2, Glut-2, liver-type glucose transporter
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20526
Homologene: 68047
Topaz1
Name: testis and ovary specific PAZ domain containing 1
Synonyms: Gm9524
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 671232
VEGA: 9
Homologene: 19835
Osgepl1
Name: O-sialoglycoprotein endopeptidase-like 1
Synonyms: 2610001M19Rik, MGC13061
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72085
Homologene: 5561
Mug1
Name: murinoglobulin 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17836
HGNC: HGNC:9750
Homologene: 136663
Kif14
Name: kinesin family member 14
Synonyms: N-3 kinesin, D1Ertd367e
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381293
Homologene: 8916
Hsdl1
Name: hydroxysteroid dehydrogenase like 1
Synonyms: 2700067E09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72552
Homologene: 100660
Cntln
Name: centlein, centrosomal protein
Synonyms: B430108F07Rik, D530005L17Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 338349
Homologene: 9805
Mbl1
Name: mannose-binding lectin (protein A) 1
Synonyms: MBL-A, MBP-A
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 17194
VEGA: 14
HGNC: HGNC:6921
Homologene: 55449
AL627093.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Cdcp2
Name: CUB domain containing protein 2
Synonyms: D030010E02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242603
Homologene: 138406
Gm7647
Name: predicted gene 7647
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108169099
Acsbg3
Name: acyl-CoA synthetase bubblegum family member 3
Synonyms: 1700061G19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 78625
Homologene: 28378
3425401B19Rik
Name: RIKEN cDNA 3425401B19 gene
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100504518
VEGA: 14
Homologene: 54908
Sorcs2
Name: sortilin-related VPS10 domain containing receptor 2
Synonyms: VPS10 domain receptor protein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81840
Homologene: 56899
Scn11a
Name: sodium channel, voltage-gated, type XI, alpha
Synonyms: NaN, NSS2, SNS2, NaT
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24046
VEGA: 9
Homologene: 8041
Clip2
Name: CAP-GLY domain containing linker protein 2
Synonyms: CLIP-115, WSCR4, Cyln2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269713
HGNC: HGNC:2586
Homologene: 20718
Lcmt2
Name: leucine carboxyl methyltransferase 2
Synonyms: Tyw4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329504
Homologene: 19642
Plekha8
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8
Synonyms: FAPP2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 231999
Homologene: 32284
Neu3
Name: neuraminidase 3
Synonyms: membrane sialidase, ganglioside sialidase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 50877
HGNC: HGNC:7760
Homologene: 4847
Or5m9
Name: olfactory receptor family 5 subfamily M member 9
Synonyms: Olfr1034, Olfr1533-ps1, MOR245-14P, GA_x6K02T2Q125-47521463-47522395, MOR227-8P, MOR227-8P
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258216
Homologene: 51741
Vmn2r95
Name: vomeronasal 2, receptor 95
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 328759
Homologene: 115024
Fgf12
Name: fibroblast growth factor 12
Synonyms: Fhf1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 14167
VEGA: 16
HGNC: HGNC:3668
Homologene: 10874
Dsn1
Name: DSN1 homolog, MIS12 kinetochore complex component
Synonyms: 1700022L09Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66934
Homologene: 49806
Zfp444
Name: zinc finger protein 444
Synonyms: 2810031J10Rik, 6230401O10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72667
Homologene: 10139
Pcdhb21
Name: protocadherin beta 21
Synonyms: PcdhbU, Pcdhb18
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93892
Homologene: 134302
Tcim
Name: transcriptional and immune response regulator
Synonyms: 1810011O10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69068
HGNC: HGNC:1357
Homologene: 10590
Togaram1
Name: TOG array regulator of axonemal microtubules 1
Synonyms: A430041B07Rik, Fam179b
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 328108
VEGA: 12
Homologene: 15025
Or1j1
Name: olfactory receptor family 1 subfamily J member 1
Synonyms: MOR136-14, Olfr3, GA_x6K02T2NLDC-33507606-33506665, Y71
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18328
HGNC: HGNC:8208
Homologene: 66579
Tmem214
Name: transmembrane protein 214
Synonyms: 1110039B18Rik, 4921530J21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68796
Homologene: 9801
1700028K03Rik
Name: RIKEN cDNA 1700028K03 gene
Synonyms: Spo16, SCRE
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76421
Homologene: 18810
Dync1i1
Name: dynein cytoplasmic 1 intermediate chain 1
Synonyms: DIC, IC74, Dncic1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13426
HGNC: HGNC:2963
Homologene: 68398
Or7g34
Name: olfactory receptor family 7 subfamily G member 34
Synonyms: Olfr854, GA_x6K02T2PVTD-13313295-13312357, MOR147-3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258515
Homologene: 128144
Dtd2
Name: D-tyrosyl-tRNA deacylase 2
Synonyms: 4930578F06Rik, 6530401N04Rik, B830049N13Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 328092
Homologene: 12634
Vcpkmt
Name: valosin containing protein lysine (K) methyltransferase
Synonyms: Gm71, LOC207965, Mettl21d
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 207965
VEGA: 12
Homologene: 56995
Or6s1
Name: olfactory receptor family 6 subfamily S member 1
Synonyms: Olfr750, GA_x6K02T2PMLR-6808276-6807281, MOR103-18
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 404319
Homologene: 19719
Ly6g6f
Name: lymphocyte antigen 6 family member G6F
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 433099
Homologene: 10938
Atg12
Name: autophagy related 12
Synonyms: 4931423H11Rik, A330058M13Rik, Apg12l
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67526
VEGA: 18
HGNC: HGNC:588
Homologene: 37953
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 53,321,096 bp
  • C to A, chromosome 1 at 136,469,147 bp
  • G to T, chromosome 1 at 152,508,561 bp
  • T to A, chromosome 1 at 156,441,246 bp
  • G to A, chromosome 2 at 5,008,545 bp
  • T to A, chromosome 2 at 36,812,615 bp
  • T to C, chromosome 2 at 76,718,282 bp
  • A to T, chromosome 2 at 86,047,067 bp
  • C to A, chromosome 2 at 121,048,895 bp
  • A to T, chromosome 2 at 121,139,344 bp
  • A to T, chromosome 2 at 156,998,713 bp
  • G to A, chromosome 3 at 28,718,816 bp
  • A to T, chromosome 4 at 11,599,809 bp
  • A to T, chromosome 4 at 82,970,633 bp
  • C to T, chromosome 4 at 85,005,053 bp
  • A to G, chromosome 4 at 107,107,192 bp
  • G to A, chromosome 4 at 123,471,320 bp
  • A to C, chromosome 4 at 128,487,005 bp
  • A to C, chromosome 5 at 30,869,668 bp
  • G to A, chromosome 5 at 36,031,190 bp
  • T to C, chromosome 5 at 94,962,980 bp
  • A to G, chromosome 5 at 107,548,201 bp
  • A to G, chromosome 5 at 134,516,151 bp
  • A to G, chromosome 6 at 6,027,399 bp
  • A to T, chromosome 6 at 54,622,107 bp
  • A to G, chromosome 6 at 121,851,424 bp
  • G to A, chromosome 7 at 6,188,173 bp
  • A to G, chromosome 7 at 19,584,164 bp
  • A to G, chromosome 7 at 65,302,921 bp
  • G to A, chromosome 7 at 99,823,317 bp
  • A to G, chromosome 7 at 120,138,149 bp
  • T to C, chromosome 8 at 24,438,635 bp
  • G to A, chromosome 8 at 119,565,711 bp
  • C to T, chromosome 8 at 124,839,929 bp
  • A to T, chromosome 9 at 19,566,949 bp
  • C to T, chromosome 9 at 24,558,095 bp
  • T to C, chromosome 9 at 35,561,203 bp
  • G to A, chromosome 9 at 42,347,892 bp
  • T to C, chromosome 9 at 83,112,248 bp
  • A to G, chromosome 9 at 96,685,773 bp
  • A to G, chromosome 9 at 104,099,451 bp
  • A to G, chromosome 9 at 106,708,299 bp
  • ACCC to ACC, chromosome 9 at 114,421,744 bp
  • A to G, chromosome 9 at 119,790,119 bp
  • C to A, chromosome 9 at 122,749,479 bp
  • A to C, chromosome 10 at 107,538,920 bp
  • T to A, chromosome 11 at 23,711,201 bp
  • A to G, chromosome 11 at 116,546,459 bp
  • A to G, chromosome 11 at 116,810,786 bp
  • G to T, chromosome 11 at 120,536,156 bp
  • T to C, chromosome 12 at 52,004,959 bp
  • T to C, chromosome 12 at 64,966,002 bp
  • T to C, chromosome 12 at 69,582,328 bp
  • T to A, chromosome 12 at 102,768,206 bp
  • T to A, chromosome 13 at 9,563,208 bp
  • A to G, chromosome 13 at 55,312,835 bp
  • A to G, chromosome 13 at 89,584,716 bp
  • A to C, chromosome 14 at 32,526,842 bp
  • A to G, chromosome 14 at 32,662,962 bp
  • A to G, chromosome 14 at 41,158,565 bp
  • T to A, chromosome 14 at 51,071,157 bp
  • T to C, chromosome 15 at 7,177,580 bp
  • A to T, chromosome 15 at 76,615,138 bp
  • A to T, chromosome 16 at 28,189,628 bp
  • C to T, chromosome 17 at 18,439,503 bp
  • T to A, chromosome 17 at 22,573,121 bp
  • T to C, chromosome 17 at 35,082,852 bp
  • T to A, chromosome 17 at 56,885,169 bp
  • C to T, chromosome 18 at 4,649,122 bp
  • A to G, chromosome 18 at 22,523,520 bp
  • T to C, chromosome 18 at 37,516,032 bp
  • T to C, chromosome 18 at 46,741,410 bp
  • T to C, chromosome 18 at 73,970,321 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0519 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038712-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.