Strain Name:
C57BL/6J-MtgxR1104Btlr/Mmmh
Stock Number:
039177-MU
Citation ID:
RRID:MMRRC_039177-MU
Other Names:
R1104 (G1), C57BL/6J-MtgxR1104Btlr
Major Collection:

Strain Information

Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, DPEAAE, Cspg2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Gsr
Name: glutathione reductase
Synonyms: Gr-1, Gr1, D8Ertd238e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14782
HGNC: HGNC:4623
Homologene: 531
Agap1
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms: Ggap1, Centg2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 347722
Homologene: 56689
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: CD221, IGF-1R, line 186, hyft, A330103N21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Znfx1
Name: zinc finger, NFX1-type containing 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 98999
Homologene: 10877
Cdca2
Name: cell division cycle associated 2
Synonyms: 2610311M19Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 108912
Homologene: 18444
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107182
Homologene: 31978
Fry
Name: FRY microtubule binding protein
Synonyms: cg003, 9330186A19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320365
Homologene: 113770
Ago1
Name: argonaute RISC catalytic subunit 1
Synonyms: argonaute 1, Eif2c1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 236511
HGNC: HGNC:3262
Homologene: 81826
Parn
Name: poly(A)-specific ribonuclease (deadenylation nuclease)
Synonyms: DAN, 1200003I18Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74108
VEGA: 16
HGNC: HGNC:8609
Homologene: 31098
Mark3
Name: MAP/microtubule affinity regulating kinase 3
Synonyms: ETK-1, 1600015G02Rik, A430080F22Rik, C-TAK1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 17169
VEGA: 12
HGNC: HGNC:6897
Homologene: 55653
Abcg5
Name: ATP binding cassette subfamily G member 5
Synonyms: Sterolin-1, trac, cmp
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 27409
Homologene: 31909
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Kdm3b
Name: KDM3B lysine (K)-specific demethylase 3B
Synonyms: 5830462I21Rik, JHDM2B, Jmjd1b
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 277250
VEGA: 18
HGNC: HGNC:1337
Homologene: 41145
Gabrr3
Name: gamma-aminobutyric acid type A receptor subunit rho 3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 328699
VEGA: 16
Homologene: 26716
B3gnt3
Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Synonyms: 2210008L19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72297
Homologene: 113516
Trappc3
Name: trafficking protein particle complex 3
Synonyms: 1110058K12Rik, Bet3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 27096
Homologene: 6399
Cul7
Name: cullin 7
Synonyms: p193, 2510004L20Rik, p185, C230011P08Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66515
Homologene: 56683
Itih5
Name: inter-alpha-trypsin inhibitor, heavy chain 5
Synonyms: 5430408M01Rik, 4631408O11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 209378
Homologene: 57115
Zfp12
Name: zinc finger protein 12
Synonyms: Krox-7, Zfp-12
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231866
Homologene: 65328
Glg1
Name: golgi apparatus protein 1
Synonyms: ESL-1, CFR, MG-160, MG160, CFR-1, Selel
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20340
HGNC: HGNC:4316
Homologene: 7533
Stau2
Name: staufen double-stranded RNA binding protein 2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 29819
Homologene: 8666
Tbl3
Name: transducin (beta)-like 3
Synonyms: 9430070M15Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 213773
Homologene: 4711
Wdr95
Name: WD40 repeat domain 95
Synonyms: 4930434E21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381693
Homologene: 124480
Ush2a
Name: usherin
Synonyms: MUSH2A, A930011D15Rik, LOC381317, LOC269160, A930037M10Rik, Ushrn, Ush2a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22283
Homologene: 66151
Liph
Name: lipase, member H
Synonyms: PLA1B, mPA-PLA1, C130037N08Rik, Lpdlr, D16Wsu119e, LPDLR
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239759
VEGA: 16
Homologene: 71802
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 672511
Homologene: 45439
Myh11
Name: myosin, heavy polypeptide 11, smooth muscle
Synonyms: smMHC, SM2, SM1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17880
VEGA: 16
HGNC: HGNC:7569
Homologene: 128512
Vmn2r6
Name: vomeronasal 2, receptor 6
Synonyms: EG620718, EG667069
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 667069
Homologene: 129754
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: GlcT-1, Epcs21, Ugcgl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22234
Homologene: 37763
Rabgap1l
Name: RAB GTPase activating protein 1-like
Synonyms: Hh1, 9630005B12Rik, 5830411O09Rik, 8430421H08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 29809
Homologene: 8143
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231470
Homologene: 23516
Trim60
Name: tripartite motif-containing 60
Synonyms: 2czf45, Rnf33
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234329
Homologene: 51876
Lrrc46
Name: leucine rich repeat containing 46
Synonyms: 1700006D24Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69297
Homologene: 12307
Dthd1
Name: death domain containing 1
Synonyms: Gm17384
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100322896
Homologene: 123510
Ivns1abp
Name: influenza virus NS1A binding protein
Synonyms: Nd1-S, Nd1-L, NS-1, ND1, HSPC068, NS1-BP, 1190004M08Rik, 1700126I16Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 117198
Homologene: 31384
Dhrs1
Name: dehydrogenase/reductase 1
Synonyms: 1110029G07Rik, dehydrogenase/reductase (SDR family) member 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 52585
Homologene: 41696
Mug2
Name: murinoglobulin 2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17837
HGNC: HGNC:9750
Homologene: 136663
Rims4
Name: regulating synaptic membrane exocytosis 4
Synonyms: Rim4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241770
Homologene: 18123
Mapkbp1
Name: mitogen-activated protein kinase binding protein 1
Synonyms: Jnkbp1, 2810483F24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 26390
Homologene: 69109
Dmrt2
Name: doublesex and mab-3 related transcription factor 2
Synonyms: Terra
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226049
VEGA: 19
HGNC: HGNC:2935
Homologene: 17111
Krt40
Name: keratin 40
Synonyms: Ka36
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 406221
Homologene: 46321
Vmn1r215
Name: vomeronasal 1 receptor 215
Synonyms: V1ri2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 171253
Homologene: 110880
Lcn11
Name: lipocalin 11
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227630
Homologene: 129702
Or2ag16
Name: olfactory receptor family 2 subfamily AG member 16
Synonyms: GA_x6K02T2PBJ9-9130754-9129519, MOR283-3, Olfr698
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258595
Homologene: 133598
Parp14
Name: poly (ADP-ribose) polymerase family, member 14
Synonyms: 1600029O10Rik, collaborator of Stat6, CoaSt6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 547253
Homologene: 19697
4930550C14Rik
Name: RIKEN cDNA 4930550C14 gene
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 75311
VEGA: 9
Homologene: 12595
Krt12
Name: keratin 12
Synonyms: K12, Krt1-12
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268482
HGNC: HGNC:6414
Homologene: 188
Flt4
Name: FMS-like tyrosine kinase 4
Synonyms: VEGFR3, VEGFR-3, Flt-4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14257
HGNC: HGNC:3767
Homologene: 7321
Cfap61
Name: cilia and flagella associated protein 61
Synonyms: 4930529M08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78774
Cryzl2
Name: crystallin zeta like 2
Synonyms: BC026585, quinone reductase-like 2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226527
Homologene: 41713
Dpf3
Name: double PHD fingers 3
Synonyms: cer-d4, 2810403B03Rik, CERD4, Gm18872
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 70127
Homologene: 129842
Rps6ka4
Name: ribosomal protein S6 kinase, polypeptide 4
Synonyms: MSK2, 1110069D02Rik, 90kDa
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56613
VEGA: 19
Homologene: 69288
Adam3
Name: ADAM metallopeptidase domain 3
Synonyms: Taz83, tMDC, Cyrn1, ADAM3, Taz83
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11497
HGNC: HGNC:209
Homologene: 69052
Hlx
Name: H2.0-like homeobox
Synonyms: Hlx1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 15284
HGNC: HGNC:4978
Homologene: 7363
Nrbf2
Name: nuclear receptor binding factor 2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 641340
Homologene: 41473
Slc17a2
Name: solute carrier family 17 (sodium phosphate), member 2
Synonyms: NPT3, C730032N17Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218103
Homologene: 4260
1700056E22Rik
Name: RIKEN cDNA 1700056E22 gene
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73363
Homologene: 137387
Ndst1
Name: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
Synonyms: glucosaminyl N-deacetylase/N-sulfotransferase 1, Hsst, 1200015G06Rik, Ndst-1, b2b2230Clo
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 15531
VEGA: 18
HGNC: HGNC:7680
Homologene: 20386
Or6c208
Name: olfactory receptor family 6 subfamily C member 208
Synonyms: GA_x6K02T2PULF-11068246-11069205, MOR110-3, Olfr784
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258724
Homologene: 138306
Gm340
Name: predicted gene 340
Synonyms: LOC381224
Type: Gene
Species: Mouse
Chromosome: 19
Cd5l
Name: CD5 antigen-like
Synonyms: AAC-11, AIM/Spalpha, Sp-alpha, Pdp 1/6, Api6, AIM
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11801
HGNC: HGNC:1690
Homologene: 55936
Dnajb14
Name: DnaJ heat shock protein family (Hsp40) member B14
Synonyms: 5730496F10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70604
Homologene: 57015
Ptgir
Name: prostaglandin I receptor (IP)
Synonyms: prostacyclin receptor, IP
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19222
HGNC: HGNC:9602
Homologene: 7496
Or51l4
Name: olfactory receptor family 51 subfamily L member 4
Synonyms: GA_x6K02T2PBJ9-6483085-6482126, MOR17-1, Olfr630
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259102
Homologene: 133674
H1f5
Name: H1.5 linker histone, cluster member
Synonyms: H1B, H1.5, H1s-3, H1f5, Hist1h1b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 56702
HGNC: HGNC:4719
Homologene: 135981
Prl3d1
Name: prolactin family 3, subfamily d, member 1
Synonyms: Pl-1, Pl1, prolactin-like 2, PL-Ia, Csh1, mPL-I, placental lactogen 1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18775
Homologene: 137215
Hus1b
Name: HUS1 checkpoint clamp component B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 210554
Homologene: 51833
Ltb4r1
Name: leukotriene B4 receptor 1
Synonyms: mBLTR, BLTR, BLT1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16995
HGNC: HGNC:6713
Homologene: 22477
Spata6l
Name: spermatogenesis associated 6 like
Synonyms: 4430402I18Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 381218
Homologene: 128454
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 16,440,361 bp
  • T to C, chromosome 1 at 89,789,240 bp
  • T to A, chromosome 1 at 118,853,350 bp
  • T to A, chromosome 1 at 151,360,109 bp
  • T to A, chromosome 1 at 157,470,604 bp
  • C to T, chromosome 1 at 160,231,875 bp
  • C to T, chromosome 1 at 184,033,505 bp
  • G to T, chromosome 1 at 184,731,987 bp
  • T to C, chromosome 1 at 188,916,256 bp
  • A to G, chromosome 2 at 10,251,512 bp
  • G to T, chromosome 2 at 25,779,103 bp
  • A to G, chromosome 2 at 120,011,073 bp
  • C to A, chromosome 2 at 145,951,061 bp
  • C to T, chromosome 2 at 163,863,929 bp
  • C to A, chromosome 2 at 167,055,640 bp
  • A to T, chromosome 3 at 64,538,066 bp
  • T to A, chromosome 3 at 87,360,899 bp
  • T to A, chromosome 3 at 137,908,354 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • C to T, chromosome 4 at 126,272,966 bp
  • T to C, chromosome 4 at 126,453,633 bp
  • A to C, chromosome 5 at 62,821,959 bp
  • G to A, chromosome 5 at 96,708,671 bp
  • A to G, chromosome 5 at 143,245,745 bp
  • G to T, chromosome 5 at 149,606,337 bp
  • A to G, chromosome 5 at 150,496,289 bp
  • C to T, chromosome 6 at 122,059,055 bp
  • G to A, chromosome 7 at 16,907,130 bp
  • T to A, chromosome 7 at 68,195,026 bp
  • A to G, chromosome 7 at 103,754,976 bp
  • A to T, chromosome 7 at 106,752,782 bp
  • T to C, chromosome 8 at 24,681,529 bp
  • A to G, chromosome 8 at 33,669,921 bp
  • A to T, chromosome 8 at 65,001,419 bp
  • A to G, chromosome 8 at 71,693,837 bp
  • A to G, chromosome 8 at 111,197,603 bp
  • A to G, chromosome 9 at 53,421,617 bp
  • A to T, chromosome 10 at 67,267,912 bp
  • T to A, chromosome 10 at 129,388,221 bp
  • C to T, chromosome 11 at 49,636,339 bp
  • C to T, chromosome 11 at 97,036,171 bp
  • C to A, chromosome 11 at 99,421,966 bp
  • T to G, chromosome 11 at 99,540,233 bp
  • T to C, chromosome 11 at 119,477,229 bp
  • A to G, chromosome 12 at 83,331,987 bp
  • C to T, chromosome 12 at 111,618,397 bp
  • C to T, chromosome 13 at 11,669,969 bp
  • T to C, chromosome 13 at 21,780,281 bp
  • T to A, chromosome 13 at 23,076,588 bp
  • T to C, chromosome 13 at 23,819,937 bp
  • C to T, chromosome 13 at 27,100,009 bp
  • T to C, chromosome 13 at 30,947,696 bp
  • T to C, chromosome 13 at 89,692,410 bp
  • T to C, chromosome 14 at 55,743,705 bp
  • T to C, chromosome 14 at 55,767,375 bp
  • T to A, chromosome 14 at 67,693,682 bp
  • A to G, chromosome 16 at 13,667,585 bp
  • A to G, chromosome 16 at 14,202,127 bp
  • A to G, chromosome 16 at 21,984,148 bp
  • A to G, chromosome 16 at 35,844,415 bp
  • T to A, chromosome 16 at 59,461,635 bp
  • A to G, chromosome 17 at 24,701,606 bp
  • T to A, chromosome 17 at 46,663,190 bp
  • A to C, chromosome 17 at 84,682,049 bp
  • T to A, chromosome 18 at 34,819,811 bp
  • T to A, chromosome 18 at 60,697,146 bp
  • T to A, chromosome 19 at 6,830,996 bp
  • T to A, chromosome 19 at 25,678,616 bp
  • T to C, chromosome 19 at 28,967,632 bp
  • T to A, chromosome 19 at 37,004,602 bp
  • G to T, chromosome 19 at 41,586,063 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1104 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039177-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.