Strain Name:
C57BL/6J-MtgxR2250Btlr/Mmmh
Stock Number:
040250-MU
Citation ID:
RRID:MMRRC_040250-MU
Other Names:
R2250 (G1), C57BL/6J-MtgxR2250Btlr
Major Collection:

Strain Information

Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21828
Homologene: 20691
Prkd3
Name: protein kinase D3
Synonyms: PKD3, 4930557O20Rik, Prkcn, 5730497N19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 75292
HGNC: HGNC:9408
Homologene: 2055
Plcb4
Name: phospholipase C, beta 4
Synonyms: A930039J07Rik, C230058B11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18798
HGNC: HGNC:9059
Homologene: 8471
Strn4
Name: striatin, calmodulin binding protein 4
Synonyms: ZIN, zinedin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 97387
Homologene: 8378
Skp1
Name: S-phase kinase-associated protein 1
Synonyms: 2610043E24Rik, Skp1a, p19Skp1, Tceb1l, 2610206H23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21402
Homologene: 38775
Mcm2
Name: minichromosome maintenance complex component 2
Synonyms: Mcmd2, BM28, CDCL1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17216
HGNC: HGNC:6944
Homologene: 3325
Abr
Name: active BCR-related gene
Synonyms: 6330400K15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 109934
HGNC: HGNC:81
Homologene: 11081
Nectin3
Name: nectin cell adhesion molecule 3
Synonyms: Pvrl3, 2610301B19Rik, nectin-3, 4921513D19Rik, 3000002N23Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 58998
Homologene: 9162
Marchf6
Name: membrane associated ring-CH-type finger 6
Synonyms: F830029L24Rik, March6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223455
VEGA: 15
Homologene: 4301
Sult3a2
Name: sulfotransferase family 3A, member 2
Synonyms: Gm4794
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215895
VEGA: 10
Homologene: 111031
Spta1
Name: spectrin alpha, erythrocytic 1
Synonyms: Spna1, ihj, erythroid, Spna-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20739
Homologene: 74460
Vnn1
Name: vanin 1
Synonyms: pantetheinase, V-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22361
Homologene: 32130
Irf2bp1
Name: interferon regulatory factor 2 binding protein 1
Synonyms: 6330414O09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 272359
Homologene: 32276
Slco6c1
Name: solute carrier organic anion transporter family, member 6c1
Synonyms: 4933404A18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74441
Homologene: 65259
Sla2
Name: Src-like-adaptor 2
Synonyms: SLAP2, A930009E21Rik, SLAP-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77799
Homologene: 49989
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: AI414108, LOC235086
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235086
Homologene: 19472
Hhatl
Name: hedgehog acyltransferase-like
Synonyms: Mitsugumin 56, Mg56, 1110011D13Rik, Gup1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74770
VEGA: 9
Homologene: 19287
Scn11a
Name: sodium channel, voltage-gated, type XI, alpha
Synonyms: NaN, NSS2, SNS2, NaT
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24046
VEGA: 9
Homologene: 8041
Mindy4
Name: MINDY lysine 48 deubiquitinase 4
Synonyms: Fam188b, LOC384387, C330043M08Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330323
Homologene: 49991
Edem2
Name: ER degradation enhancer, mannosidase alpha-like 2
Synonyms: 9530090G24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 108687
Homologene: 10075
Lyg2
Name: lysozyme G-like 2
Synonyms: LOC332427
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 332427
Homologene: 18483
Vmn1r119
Name: vomeronasal 1 receptor 119
Synonyms: LOC384696, Gm1447
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384696
Homologene: 104166
Or6b9
Name: olfactory receptor family 6 subfamily B member 9
Synonyms: M50, GA_x6K02T2PBJ9-9337331-9336381, Olfr6, MOR103-16
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233670
Homologene: 28439
Or52e2
Name: olfactory receptor family 52 subfamily E member 2
Synonyms: MOR32-3, GA_x6K02T2PBJ9-5871256-5870303, Olfr589
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259054
Homologene: 133046
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 37,915,735 bp
  • A to G, chromosome 1 at 97,062,360 bp
  • A to G, chromosome 1 at 174,244,114 bp
  • G to A, chromosome 2 at 135,971,861 bp
  • A to C, chromosome 2 at 155,710,973 bp
  • G to A, chromosome 2 at 156,875,942 bp
  • G to A, chromosome 6 at 55,300,949 bp
  • G to A, chromosome 6 at 88,893,008 bp
  • A to G, chromosome 7 at 16,826,466 bp
  • G to T, chromosome 7 at 19,005,799 bp
  • A to T, chromosome 7 at 21,012,259 bp
  • C to A, chromosome 7 at 103,154,950 bp
  • T to A, chromosome 7 at 106,956,373 bp
  • G to A, chromosome 9 at 27,309,478 bp
  • T to A, chromosome 9 at 119,758,602 bp
  • A to G, chromosome 9 at 121,788,171 bp
  • A to T, chromosome 10 at 23,900,572 bp
  • T to G, chromosome 10 at 33,768,167 bp
  • T to C, chromosome 11 at 52,243,619 bp
  • A to G, chromosome 11 at 76,451,939 bp
  • A to G, chromosome 13 at 92,762,750 bp
  • G to A, chromosome 15 at 31,484,935 bp
  • A to T, chromosome 16 at 46,454,736 bp
  • T to C, chromosome 17 at 78,968,078 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2250 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040250-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.