Strain Name:
C57BL/6J-MtgxR2280Btlr/Mmmh
Stock Number:
040279-MU
Citation ID:
RRID:MMRRC_040279-MU
Other Names:
R2280 (G1), C57BL/6J-MtgxR2280Btlr
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: SOW3, Dominant white spotting, c-KIT, Steel Factor Receptor, CD117, belly-spot, Tr-kit, Gsfsow3, Gsfsco5, SCO1, SCO5, Gsfsco1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Npy1r
Name: neuropeptide Y receptor Y1
Synonyms: Y1-R, Npyr
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18166
HGNC: HGNC:7956
Homologene: 700
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, ne, b2b1562Clo, 6030440P17Rik, my
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Mthfd1
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms: DCS, Mthfd, E430024A07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 108156
VEGA: 12
HGNC: HGNC:7432
Homologene: 55940
Utp20
Name: UTP20 small subunit processome component
Synonyms: DRIM, 3830408P06Rik, mDRIM
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Clasp1
Name: CLIP associating protein 1
Synonyms: CLASP1alpha, CLASP1, mCLASP1, 1700030C23Rik, 5730583A19Rik, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Pum1
Name: pumilio RNA-binding family member 1
Synonyms: Pumm
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 80912
Homologene: 22830
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: slip, DNAPDcs, DNA-PKcs, DNA-PK, DOXNPH, XRCC7, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 9030227G01Rik, 2510002A14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Pip5k1b
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 beta
Synonyms: Pipk5b
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18719
HGNC: HGNC:8995
Homologene: 100644
Morf4l1
Name: mortality factor 4 like 1
Synonyms: TEG-189, MORFRG15, Tex189, MRG15
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21761
Homologene: 86043
Zfp532
Name: zinc finger protein 532
Synonyms: C530030I18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 328977
Homologene: 138627
Brd7
Name: bromodomain containing 7
Synonyms: BP75, CELTIX1, bromodomain protein 75 kDa
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 26992
Homologene: 8085
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: G431004K08Rik, GCN1L, Gcn1l1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Ddx4
Name: DEAD box helicase 4
Synonyms: VASA, mvh / m'vasa, Mvh, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13206
VEGA: 13
Homologene: 49227
Ap3b1
Name: adaptor-related protein complex 3, beta 1 subunit
Synonyms: AP-3, Hps2, beta3A, recombination induced mutation 2, rim2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 11774
VEGA: 13
HGNC: HGNC:566
Homologene: 68125
Cep170
Name: centrosomal protein 170
Synonyms: 4933426L22Rik, A330004A13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 545389
Homologene: 22844
Herc2
Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms: D7H15F37S1, D7H15F32S1, rjs, jdf2, D15F32S1h
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15204
HGNC: HGNC:4868
Homologene: 3430
Cc2d2a
Name: coiled-coil and C2 domain containing 2A
Synonyms: 5730509K17Rik, b2b1035Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231214
Homologene: 18159
Ei24
Name: etoposide induced 2.4 mRNA
Synonyms: PIG8
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13663
Homologene: 3588
Iars2
Name: isoleucine-tRNA synthetase 2, mitochondrial
Synonyms: 2010002H18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381314
Homologene: 7118
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Ganab
Name: alpha glucosidase 2 alpha neutral subunit
Synonyms: G2an, GluII
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14376
VEGA: 19
HGNC: HGNC:4138
Homologene: 5426
Pcx
Name: pyruvate carboxylase
Synonyms: Pc
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18563
VEGA: 19
HGNC: HGNC:8636
Homologene: 5422
Gbx2
Name: gastrulation brain homeobox 2
Synonyms: MMoxA, Stra7, Gbx-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14472
HGNC: HGNC:4186
Homologene: 1138
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: Plc, perlecan, Pcn, per
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Zfp12
Name: zinc finger protein 12
Synonyms: Zfp-12, Krox-7
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231866
Homologene: 65328
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: 9330197E15Rik, A630028O16Rik, LOC380767
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 211978
VEGA: 12
Homologene: 9102
Scamp5
Name: secretory carrier membrane protein 5
Synonyms: Sc5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56807
VEGA: 9
Homologene: 56880
Slc12a9
Name: solute carrier family 12 (potassium/chloride transporters), member 9
Synonyms: CIP1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 83704
Homologene: 5429
Fbxl19
Name: F-box and leucine-rich repeat protein 19
Synonyms: Fbl19
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233902
Homologene: 129692
Clvs2
Name: clavesin 2
Synonyms: Rlbp1l2, A330019N05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215890
Homologene: 27894
Rcsd1
Name: RCSD domain containing 1
Synonyms: A430105K13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226594
Homologene: 33475
Txnip
Name: thioredoxin interacting protein
Synonyms: VDUP1, THIF, mVDUP1, Hyplip1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56338
Homologene: 38186
Gm872
Name: predicted gene 872
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Sntg1
Name: syntrophin, gamma 1
Synonyms: G1SYN, SYN4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71096
Homologene: 56834
Dcdc5
Name: doublecortin domain containing 5
Synonyms: 4732421G10Rik, EG436559
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329482
Homologene: 79722
Ccser1
Name: coiled-coil serine rich 1
Synonyms: Fam190a, C130092O11Rik, 6230405M12Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232035
Homologene: 28086
Fgd5
Name: FYVE, RhoGEF and PH domain containing 5
Synonyms: ZFYVE23, C330025N11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232237
Homologene: 27798
Ccdc168
Name: coiled-coil domain containing 168
Synonyms: Gm8251
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 102636082
VEGA: 1
Homologene: 141149
D17H6S56E-5
Name: DNA segment, Chr 17, human D6S56E 5
Synonyms: G7e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 110956
VEGA: 17
Ecsit
Name: ECSIT signalling integrator
Synonyms: Sitpec
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 26940
Homologene: 8080
Dis3l
Name: DIS3 like exosome 3'-5' exoribonuclease
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 213550
Homologene: 15797
Pxdn
Name: peroxidasin
Synonyms: VPO1, 2310075M15Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 69675
Homologene: 33907
Rsph4a
Name: radial spoke head 4 homolog A (Chlamydomonas)
Synonyms: Rshl3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 212892
Homologene: 71779
Chaf1b
Name: chromatin assembly factor 1, subunit B
Synonyms: CAF-1 subunit B, CAF-IP60, 2600017H24Rik, CAF1, MPHOSPH7, CAF1P60, CAF-I 60 kDa subunit, CAF1A
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110749
HGNC: HGNC:1911
Homologene: 48346
Tpsg1
Name: tryptase gamma 1
Synonyms: TMT, Prss31
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26945
Homologene: 74994
Taar7f
Name: trace amine-associated receptor 7F
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 435207
Homologene: 134040
Flnc
Name: filamin C, gamma
Synonyms: 1110055E19Rik, actin binding protein 280, Fln2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 68794
HGNC: HGNC:3756
Homologene: 37481
Plch2
Name: phospholipase C, eta 2
Synonyms: A930027K05Rik, PLCeta2, Plcl4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269615
Homologene: 85172
Caskin2
Name: CASK-interacting protein 2
Synonyms: 1600028L06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 140721
Homologene: 32485
Apoh
Name: apolipoprotein H
Synonyms: beta-2-glycoprotein 1, beta-2-GPI, B2GPI
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11818
HGNC: HGNC:616
Homologene: 26
Tmem216
Name: transmembrane protein 216
Synonyms: 2810441K11Rik, 4921533J23Rik, 1110017C22Rik, A930021F15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 68642
Homologene: 9541
Cyp46a1
Name: cytochrome P450, family 46, subfamily a, polypeptide 1
Synonyms: Cyp46, cholestrol 24-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13116
VEGA: 12
HGNC: HGNC:2641
Homologene: 134501
Cacna2d4
Name: calcium channel, voltage-dependent, alpha 2/delta subunit 4
Synonyms: 5730412N02Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319734
Homologene: 26544
Gcc2
Name: GRIP and coiled-coil domain containing 2
Synonyms: 2600014C01Rik, 0610043A03Rik, 2210420P05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70297
Homologene: 45639
Pde6a
Name: phosphodiesterase 6A, cGMP-specific, rod, alpha
Synonyms: Pdea
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225600
HGNC: HGNC:8785
Homologene: 380
Spmap2l
Name: sperm microtubule associated protein 2 like
Synonyms: Thegl, 1700023E05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71868
Homologene: 128474
Arhgef19
Name: Rho guanine nucleotide exchange factor 19
Synonyms: 6430573B13Rik, WGEF
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 213649
Homologene: 17710
Nphp4
Name: nephronophthisis 4 (juvenile) homolog (human)
Synonyms: 4930564O18Rik, nmf192
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 260305
Homologene: 9024
Ano3
Name: anoctamin 3
Synonyms: B230324K02Rik, Tmem16c
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228432
Homologene: 57147
Wdr35
Name: WD repeat domain 35
Synonyms: 4930459M12Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 74682
Homologene: 10814
Lrrc3b
Name: leucine rich repeat containing 3B
Synonyms: LRP15
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218763
VEGA: 14
Homologene: 14190
Cma2
Name: chymase 2, mast cell
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 545055
VEGA: 14
Homologene: 115745
Ankrd37
Name: ankyrin repeat domain 37
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 654824
Homologene: 35347
Ncoa4-ps
Name: nuclear receptor coactivator 4, pseudogene
Synonyms: Gm6768
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 627557
VEGA: 12
Or10aa1
Name: olfactory receptor family 10 subfamily AA member 1
Synonyms: Olfr433, GA_x6K02T2P20D-21133014-21132070, MOR123-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 258712
Homologene: 133588
Anks1b
Name: ankyrin repeat and sterile alpha motif domain containing 1B
Synonyms: AIDA-1b, LOC380650, E530015N03Rik, Gm10937, C030032C09Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77531
Homologene: 51570
Sike1
Name: suppressor of IKBKE 1
Synonyms: 2810005O12Rik, 5730470L24Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66641
Homologene: 32622
Gpr18
Name: G protein-coupled receptor 18
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110168
VEGA: 14
HGNC: HGNC:4472
Homologene: 18814
Crtac1
Name: cartilage acidic protein 1
Synonyms: Crtac1B, Lotus, 2810454P21Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72832
VEGA: 19
Homologene: 23070
Cox8c
Name: cytochrome c oxidase subunit 8C
Synonyms: COX8-3, COXVIII-3, 1700007F21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 75483
VEGA: 12
Homologene: 83496
Krtap19-5
Name: keratin associated protein 19-5
Synonyms: Krtap8-2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16704
VEGA: 16
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 8,595,402 bp
  • T to A, chromosome 1 at 44,056,460 bp
  • A to T, chromosome 1 at 89,930,637 bp
  • C to T, chromosome 1 at 118,565,183 bp
  • C to T, chromosome 1 at 165,659,429 bp
  • C to A, chromosome 1 at 174,042,521 bp
  • A to T, chromosome 1 at 176,774,505 bp
  • A to G, chromosome 1 at 185,323,171 bp
  • G to A, chromosome 2 at 106,372,522 bp
  • T to A, chromosome 2 at 110,682,759 bp
  • A to G, chromosome 3 at 53,572,423 bp
  • C to G, chromosome 3 at 96,559,447 bp
  • A to G, chromosome 3 at 102,997,378 bp
  • A to T, chromosome 4 at 130,766,011 bp
  • A to G, chromosome 4 at 137,522,043 bp
  • G to A, chromosome 4 at 141,246,516 bp
  • A to G, chromosome 4 at 152,557,043 bp
  • A to T, chromosome 4 at 154,984,309 bp
  • C to T, chromosome 5 at 43,737,481 bp
  • T to C, chromosome 5 at 73,041,364 bp
  • T to C, chromosome 5 at 75,637,283 bp
  • T to C, chromosome 5 at 77,059,367 bp
  • T to A, chromosome 5 at 115,612,730 bp
  • A to G, chromosome 5 at 137,332,212 bp
  • A to G, chromosome 5 at 143,245,493 bp
  • G to A, chromosome 6 at 29,438,666 bp
  • A to T, chromosome 6 at 61,570,815 bp
  • A to C, chromosome 6 at 84,064,494 bp
  • A to G, chromosome 6 at 85,677,973 bp
  • G to A, chromosome 6 at 91,988,945 bp
  • A to T, chromosome 6 at 119,350,041 bp
  • A to C, chromosome 7 at 56,137,271 bp
  • A to T, chromosome 7 at 127,748,368 bp
  • T to C, chromosome 8 at 45,999,376 bp
  • C to T, chromosome 8 at 66,704,059 bp
  • A to G, chromosome 8 at 88,342,757 bp
  • C to T, chromosome 9 at 22,076,540 bp
  • A to G, chromosome 9 at 36,782,339 bp
  • A to G, chromosome 9 at 57,445,439 bp
  • T to A, chromosome 9 at 64,317,794 bp
  • A to G, chromosome 9 at 90,107,229 bp
  • G to A, chromosome 10 at 24,049,519 bp
  • A to T, chromosome 10 at 33,528,500 bp
  • A to T, chromosome 10 at 33,911,599 bp
  • C to A, chromosome 10 at 58,269,680 bp
  • A to T, chromosome 10 at 88,825,503 bp
  • A to C, chromosome 10 at 90,966,302 bp
  • A to G, chromosome 10 at 92,885,130 bp
  • T to A, chromosome 11 at 108,409,180 bp
  • G to T, chromosome 11 at 115,802,989 bp
  • A to G, chromosome 12 at 8,978,628 bp
  • T to C, chromosome 12 at 29,984,906 bp
  • T to C, chromosome 12 at 76,280,492 bp
  • T to A, chromosome 12 at 79,275,040 bp
  • A to T, chromosome 12 at 102,899,454 bp
  • T to C, chromosome 12 at 108,355,471 bp
  • A to G, chromosome 12 at 119,262,838 bp
  • A to G, chromosome 13 at 94,528,216 bp
  • T to C, chromosome 13 at 112,620,656 bp
  • A to T, chromosome 14 at 15,358,076 bp
  • T to A, chromosome 14 at 55,971,528 bp
  • T to A, chromosome 14 at 121,912,605 bp
  • A to G, chromosome 16 at 15,725,471 bp
  • C to T, chromosome 16 at 88,896,343 bp
  • T to G, chromosome 16 at 93,891,571 bp
  • C to T, chromosome 17 at 25,374,042 bp
  • A to G, chromosome 17 at 34,998,755 bp
  • T to C, chromosome 18 at 61,262,434 bp
  • T to C, chromosome 18 at 65,624,712 bp
  • C to A, chromosome 19 at 4,604,543 bp
  • T to C, chromosome 19 at 8,909,468 bp
  • T to A, chromosome 19 at 10,551,873 bp
  • T to A, chromosome 19 at 24,378,947 bp
  • G to A, chromosome 19 at 42,283,567 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2280 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040279-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.