Strain Name:
C57BL/6J-MtgxR2474Btlr/Mmmh
Stock Number:
040405-MU
Citation ID:
RRID:MMRRC_040405-MU
Other Names:
R2474 (G1), C57BL/6J-MtgxR2474Btlr
Major Collection:

Strain Information

Pik3r1
Name: phosphoinositide-3-kinase regulatory subunit 1
Synonyms: p55alpha, p85alpha, p50alpha, PI3K
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18708
HGNC: HGNC:8979
Homologene: 7889
Phb1
Name: prohibitin 1
Synonyms: Phb
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18673
HGNC: HGNC:8912
Homologene: 1980
Tfap2b
Name: transcription factor AP-2 beta
Synonyms: AP-2(beta), E130018K07Rik, Tcfap2b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21419
Homologene: 20688
Lgi3
Name: leucine-rich repeat LGI family, member 3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 213469
VEGA: 14
Homologene: 16406
Senp3
Name: SUMO/sentrin specific peptidase 3
Synonyms: Smt3ip1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 80886
Homologene: 9236
Secisbp2l
Name: SECIS binding protein 2-like
Synonyms: 3110001I20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70354
Homologene: 18923
Nedd1
Name: neural precursor cell expressed, developmentally down-regulated gene 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17997
VEGA: 10
HGNC: HGNC:7723
Homologene: 7439
Adgrl4
Name: adhesion G protein-coupled receptor L4
Synonyms: EGF-TM7 receptor, 1110033N21Rik, Etl, Eltd1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 170757
Homologene: 11170
Rps5
Name: ribosomal protein S5
Synonyms: S5 ribosomal protein
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20103
Homologene: 783
Zfp746
Name: zinc finger protein 746
Synonyms: 2810407L07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 69228
Homologene: 19783
Cxcl13
Name: C-X-C motif chemokine ligand 13
Synonyms: BLC, Scyb13, 4631412M08Rik, ANGIE2, BCA-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 55985
Homologene: 48431
Itpkb
Name: inositol 1,4,5-trisphosphate 3-kinase B
Synonyms: E130307H12Rik, 1110033J02Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 320404
HGNC: HGNC:6179
Homologene: 1672
Dchs1
Name: dachsous cadherin related 1
Synonyms: C130033F22Rik, 3110041P15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233651
Homologene: 2771
Enpep
Name: glutamyl aminopeptidase
Synonyms: Ly51, aminopeptidase-A, 6030431M22Rik, APA, Bp-1/6C3, Ly-51
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 13809
HGNC: HGNC:3355
Homologene: 68216
Hfm1
Name: HFM1, ATP-dependent DNA helicase homolog
Synonyms: Mer3, LOC381663, Sec63d1, A330009G12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 330149
Homologene: 87103
Vmn2r84
Name: vomeronasal 2, receptor 84
Synonyms: EG625068
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 625068
Homologene: 129606
Greb1
Name: gene regulated by estrogen in breast cancer protein
Synonyms: 5730583K22Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 268527
Homologene: 8780
Mpeg1
Name: macrophage expressed gene 1
Synonyms: Mpg-1, Perforin-2, MPS1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 17476
VEGA: 19
Homologene: 11216
Tmem260
Name: transmembrane protein 260
Synonyms: 6720456H20Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218989
Homologene: 49506
Vmn2r99
Name: vomeronasal 2, receptor 99
Synonyms: EG665376
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 665376
Homologene: 115024
Klk14
Name: kallikrein related-peptidase 14
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 317653
HGNC: HGNC:6362
Homologene: 69348
Or8g26
Name: olfactory receptor family 8 subfamily G member 26
Synonyms: Olfr943, GA_x6K02T2PVTD-32881408-32882343, MOR171-44
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258323
VEGA: 9
HGNC: HGNC:8484
Homologene: 110526
Parp8
Name: poly (ADP-ribose) polymerase family, member 8
Synonyms: D13Ertd275e, 2810430O08Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 52552
VEGA: 13
Homologene: 11621
Ilvbl
Name: ilvB (bacterial acetolactate synthase)-like
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216136
VEGA: 10
HGNC: HGNC:6041
Homologene: 68532
Zw10
Name: zw10 kinetochore protein
Synonyms: MmZw10, 6330566F14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 26951
VEGA: 9
Homologene: 37959
Asb7
Name: ankyrin repeat and SOCS box-containing 7
Synonyms: D030055C23Rik, Asb-7
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 117589
Homologene: 34130
Or8k35
Name: olfactory receptor family 8 subfamily K member 35
Synonyms: MOR192-4_p, GA_x6K02T2Q125-48079993-48079157, Olfr1082
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 404473
Homologene: 104285
Atxn7l2
Name: ataxin 7-like 2
Synonyms: 2610528J18Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72522
Homologene: 17728
4921506M07Rik
Name: RIKEN cDNA 4921506M07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Bckdk
Name: branched chain ketoacid dehydrogenase kinase
Synonyms: BCKD-kinase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12041
Homologene: 37642
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 19,214,375 bp
  • A to G, chromosome 1 at 180,334,151 bp
  • C to A, chromosome 2 at 86,594,613 bp
  • C to T, chromosome 2 at 125,740,737 bp
  • A to C, chromosome 3 at 108,203,977 bp
  • G to C, chromosome 3 at 129,284,158 bp
  • A to G, chromosome 3 at 151,542,724 bp
  • C to T, chromosome 5 at 95,959,957 bp
  • A to T, chromosome 5 at 106,872,416 bp
  • T to C, chromosome 6 at 48,064,769 bp
  • T to A, chromosome 7 at 12,926,561 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • A to T, chromosome 7 at 66,679,153 bp
  • T to C, chromosome 7 at 105,755,074 bp
  • A to T, chromosome 7 at 105,772,838 bp
  • C to A, chromosome 7 at 127,905,418 bp
  • A to G, chromosome 9 at 39,184,550 bp
  • T to A, chromosome 9 at 49,066,805 bp
  • T to C, chromosome 10 at 78,576,724 bp
  • A to G, chromosome 10 at 92,719,603 bp
  • A to T, chromosome 10 at 130,386,523 bp
  • T to A, chromosome 11 at 69,674,097 bp
  • T to C, chromosome 11 at 95,671,422 bp
  • T to C, chromosome 12 at 16,714,953 bp
  • G to T, chromosome 12 at 57,575,927 bp
  • A to T, chromosome 13 at 101,702,776 bp
  • A to T, chromosome 13 at 116,893,041 bp
  • A to G, chromosome 14 at 48,496,324 bp
  • G to A, chromosome 14 at 70,533,249 bp
  • A to T, chromosome 17 at 19,378,629 bp
  • G to A, chromosome 19 at 12,462,249 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2474 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040405-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.