Strain Name:
C57BL/6J-MtgxR4297Btlr/Mmmh
Stock Number:
041085-MU
Citation ID:
RRID:MMRRC_041085-MU
Other Names:
R4297 (G1), C57BL/6J-MtgxR4297Btlr
Major Collection:

Strain Information

Camkk2
Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
Synonyms: 6330570N16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 207565
HGNC: HGNC:1470
Homologene: 32756
Setx
Name: senataxin
Synonyms: Als4, A930037J23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269254
HGNC: HGNC:445
Homologene: 41003
Ctdp1
Name: CTD phosphatase subunit 1
Synonyms: 4930563P03Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67655
HGNC: HGNC:2498
Homologene: 31254
Epcam
Name: epithelial cell adhesion molecule
Synonyms: gp40, panepithelial glycoprotein 314, EpCAM1, Ly74, Egp314, EpCAM, Tacstd1, Ep-CAM, EGP-2, GA733-2, TROP1, CD326
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17075
VEGA: 17
Homologene: 1764
Mc5r
Name: melanocortin 5 receptor
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17203
VEGA: 18
HGNC: HGNC:6933
Homologene: 4321
Ncapd3
Name: non-SMC condensin II complex, subunit D3
Synonyms: 2810487N22Rik, B130055D15Rik, 4632407J06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 78658
VEGA: 9
Homologene: 41021
Vps18
Name: VPS18 CORVET/HOPS core subunit
Synonyms: 9930024E13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228545
Homologene: 13302
Creld2
Name: cysteine-rich with EGF-like domains 2
Synonyms: 5730592L21Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76737
VEGA: 15
Homologene: 11526
Glipr1l1
Name: GLI pathogenesis-related 1 like 1
Synonyms: 1700011E04Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 69286
VEGA: 10
Homologene: 83452
Dgke
Name: diacylglycerol kinase, epsilon
Synonyms: DAGK6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56077
HGNC: HGNC:2852
Homologene: 2705
Cep295
Name: centrosomal protein 295
Synonyms: LOC382128, 5830418K08Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319675
Homologene: 27936
Gprc5b
Name: G protein-coupled receptor, family C, group 5, member B
Synonyms: hypothetical protein, clone 2-63
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 64297
Homologene: 9435
Cfap46
Name: cilia and flagella associated protein 46
Synonyms: Ttc40, 9330101J02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 212124
Vax1
Name: ventral anterior homeobox 1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22326
Homologene: 7593
Reln
Name: reelin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Wdr11
Name: WD repeat domain 11
Synonyms: 2900055P10Rik, Brwd2, Wdr11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 207425
Homologene: 41229
Col6a3
Name: collagen, type VI, alpha 3
Synonyms: Col6a-3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12835
HGNC: HGNC:2213
Homologene: 37917
Abcc12
Name: ATP-binding cassette, sub-family C member 12
Synonyms: 4930467B22Rik, MRP9
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244562
Homologene: 57211
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: alpha1E, Cchra1, Cav2.3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Col5a1
Name: collagen, type V, alpha 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12831
HGNC: HGNC:2209
Homologene: 55434
Slc6a21
Name: solute carrier family 6 member 21
Synonyms: 1700039E15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76713
Homologene: 66347
Ugt1a8
Name: UDP glucuronosyltransferase 1 family, polypeptide A8
Synonyms: OTTMUSG00000020811, A116
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 613123
Cfap43
Name: cilia and flagella associated protein 43
Synonyms: 4632415N18Rik, D19Ertd652e, 4930428C11Rik, Wdr96, 4930463G05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 100048534
Homologene: 36425
Vcam1
Name: vascular cell adhesion molecule 1
Synonyms: CD106, Vcam-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 22329
Homologene: 838
Fpr-rs3
Name: formyl peptide receptor, related sequence 3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14290
HGNC: HGNC:3827
Homologene: 130087
Cyp2a4
Name: cytochrome P450, family 2, subfamily a, polypeptide 4
Synonyms: Cyp15a1, testosterone 15alpha-hydroxylase, D7Ucla4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13086
Homologene: 85917
Vmn2r12
Name: vomeronasal 2, receptor 12
Synonyms: Gm6769
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 627569
Homologene: 129606
Alkbh3
Name: alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase
Synonyms: 1700108H04Rik, mABH3, Abh3, 1810020C19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69113
Homologene: 16393
St3gal2
Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 2
Synonyms: Siat5, ST3GalII
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20444
Homologene: 5047
Rps6-ps4
Name: ribosomal protein S6, pseudogene 4
Synonyms: Gm13654
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 105244208
Gm6818
Name: predicted gene 6818
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 627975
Homologene: 80005
Hbb-bs
Name: hemoglobin, beta adult s chain
Synonyms: beta s
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100503605
Homologene: 68066
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 88,088,104 bp
  • T to A, chromosome 1 at 90,811,378 bp
  • T to G, chromosome 1 at 154,398,731 bp
  • T to C, chromosome 2 at 28,017,204 bp
  • GTGGCT to GT, chromosome 2 at 29,154,061 bp
  • T to C, chromosome 2 at 75,191,561 bp
  • C to A, chromosome 2 at 76,726,263 bp
  • C to T, chromosome 2 at 94,008,124 bp
  • T to A, chromosome 2 at 119,297,331 bp
  • A to T, chromosome 3 at 116,117,243 bp
  • A to C, chromosome 5 at 21,920,487 bp
  • C to A, chromosome 5 at 109,091,964 bp
  • A to G, chromosome 5 at 122,745,706 bp
  • A to G, chromosome 7 at 26,307,368 bp
  • A to T, chromosome 7 at 38,402,453 bp
  • G to A, chromosome 7 at 45,287,762 bp
  • T to C, chromosome 7 at 103,826,744 bp
  • G to A, chromosome 7 at 118,984,214 bp
  • A to G, chromosome 7 at 129,625,186 bp
  • A to C, chromosome 7 at 139,652,673 bp
  • T to C, chromosome 8 at 86,531,525 bp
  • T to C, chromosome 8 at 110,962,359 bp
  • A to G, chromosome 9 at 15,322,654 bp
  • A to G, chromosome 9 at 27,052,327 bp
  • A to T, chromosome 10 at 112,062,347 bp
  • A to T, chromosome 11 at 89,050,730 bp
  • T to C, chromosome 15 at 88,823,753 bp
  • A to T, chromosome 17 at 20,624,746 bp
  • T to C, chromosome 17 at 87,640,534 bp
  • T to C, chromosome 18 at 68,339,307 bp
  • A to T, chromosome 18 at 80,449,957 bp
  • A to C, chromosome 19 at 47,876,496 bp
  • G to T, chromosome 19 at 59,166,251 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4297 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041085-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.