Strain Detail Sheet

Strain Name:
STOCK Sycp2ltm1Jw/Mmjax
Stock Number:
041440-JAX
Citation ID:
RRID:MMRRC_041440-JAX
Other Names:
Sycp2l

Strain Information

Gene Details

Sycp2ltm1Jw
Name: synaptonemal complex protein 2-like; targeted mutation 1, Jeremy Wang
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
Sycp2l
Name: synaptonemal complex protein 2-like
Synonyms: EG621792, Gm40956, LOC218175
Type: Gene
Species: Mouse
Chromosome: 13
Alteration at locus: Knockout
NCBI: 637277
Homologene: 52882
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Genetic Alterations
Sycp2l is located on Chr 13 and consists of 21 exons. In the targeted allele, deletion of exons 8-14 (encoding residues 191-383) leads to a frameshift in the transcript.Homozygous female Sycp2l knockout mice exhibit a progressive loss of oocytes and decreased fertility. These mice may be useful in the study of female reproductive aging.
Genotype Determination
ES Cell Line
V6.5

Strain Description

Phenotype
SYCP2L (synaptonemal complex protein 2-like) is a sequence homolog of SYCP2 and is expressed only in oocytes. Variants in human SYCP2L are associated with Age at Natural Menopause (ANM). SYCP2L localizes to the centromere of dictyate oocytes. The Sycp2l knockout allele has a loxP-flanked PGK neo cassette replacing exons 8-14. Homozygous female mice undergo a progressive reduction of ooctyes with aging; by 14 months oocyte numbers drop to 20% of controls. Primordial follicle number decreases beginning at 2 months of age; decreases in numbers of primary and secondary follicles are apparent at 12 months of age. Although homozygous female mice produce the same number of litters as controls, litter size is significantly decreased. Male fertility is unaffected. These mice may be useful in the study of female reproductive aging.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • Aging/genetics
  • Alleles
  • Animals
  • Cell Culture Techniques
  • Centromere/metabolism
  • Chromosomal Proteins, Non-Histone/deficiency
  • Chromosomal Proteins, Non-Histone/genetics
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • Female
  • Fertility/genetics
  • Humans
  • Menopause/genetics
  • Menopause/metabolism
  • Menopause/physiology
  • Mice
  • Oocytes/metabolism
  • Ovarian Follicle/metabolism
  • Ovary/cytology
  • Ovary/metabolism
  • Ovary/physiology
  • Ovulation/physiology
  • Polymorphism, Single Nucleotide
  • Reproduction/genetics
Strain Development
A targeting vector was designed by Dr. Jeremy Wang (University of Pennsylvania) to replace exons 8-14 of the synaptonemal complex protein 2-like gene (Sycp2l) on chromosome 13 with a loxP-flanked PGK-neo cassette leading to a frameshift in the transcript. The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to establish the colony. The resulting mice were bred with C57BL/6 wildtype mice for one generation prior to sending males to The Jackson Laboratory Repository MMRRC in 2015. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Reproduction

Strain Origin

Donor
P. Jeremy Wang, Ph.D., University of Pennsylvania.
Primary Reference

Zhou J, Stein P, Leu NA, Chm tal L, Xue J, Ma J, Huang X, Lampson MA, Schultz RM, Wang PJ. Accelerated reproductive aging in females lacking a novel centromere protein SYCP2L. 2015 Nov 15;24(22):6505-14. Epub 2015 Sep 11. (Medline PMID: 26362258)

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Mixed, black, or agouti

Breeding

MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Heterozygous female x Heterozygous male littermate
  • Homozygous female x Homozygous male littermate
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Reduced Yes Reduced fertile period in homozygous females due to accelerated reproductive aging.
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months Undetermined
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.