Strain Name:
C57BL/6J-MtgxR4497Btlr/Mmmh
Stock Number:
041750-MU
Citation ID:
RRID:MMRRC_041750-MU
Other Names:
R4497 (G1), C57BL/6J-MtgxR4497Btlr
Major Collection:

Strain Information

Trmo
Name: tRNA methyltransferase O
Synonyms: 5830415F09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74753
Homologene: 32316
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: slip, DNAPDcs, DNA-PKcs, DNA-PK, DOXNPH, XRCC7, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Birc2
Name: baculoviral IAP repeat-containing 2
Synonyms: mcIAP1, cIAP1, HIAP1, MIHB, Api1, MIAP1, IAP1, cIAP-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11797
HGNC: HGNC:590
Homologene: 900
Fbxl3
Name: F-box and leucine-rich repeat protein 3
Synonyms: Fbxl3a, Play68, Ovtm, Fbl3a
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50789
Homologene: 8127
Aco2
Name: aconitase 2, mitochondrial
Synonyms: Irp1, Aco-2, Aco3, D10Wsu183e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11429
HGNC: HGNC:118
Homologene: 856
Arhgap12
Name: Rho GTPase activating protein 12
Synonyms: 2810011M08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 75415
Homologene: 23089
Stk11ip
Name: serine/threonine kinase 11 interacting protein
Synonyms: 1200014D22Rik, LKB1IP, LIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71728
Homologene: 12406
Rorb
Name: RAR-related orphan receptor beta
Synonyms: Rorbeta, hstp, RZR-beta, Nr1f2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225998
Homologene: 38250
Itprid2
Name: ITPR interacting domain containing 2
Synonyms: Ssfa2, SPAG13, KRAP, CS1, CS-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70599
Homologene: 4912
Wdr33
Name: WD repeat domain 33
Synonyms: WDC146, 2310011G05Rik, 8430413N20Rik, 2810021O11Rik, 1110001N06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 74320
VEGA: 18
Homologene: 56807
Lamb2
Name: laminin, beta 2
Synonyms: Lams, npht, Lamb-2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 16779
HGNC: HGNC:6487
Homologene: 1723
Ccdc92
Name: coiled-coil domain containing 92
Synonyms: D5Bwg0834e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 215707
Homologene: 11848
Adat1
Name: adenosine deaminase, tRNA-specific 1
Synonyms: mADAT1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 30947
HGNC: HGNC:228
Homologene: 8096
Pgr
Name: progesterone receptor
Synonyms: PR-A, 9930019P03Rik, ENSMUSG00000074510, NR3C3, PR, PR-B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18667
HGNC: HGNC:8910
Homologene: 713
Zbtb18
Name: zinc finger and BTB domain containing 18
Synonyms: Zfp238, RP58
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 30928
Homologene: 21276
Reck
Name: reversion-inducing-cysteine-rich protein with kazal motifs
Synonyms: St15
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 53614
Homologene: 9622
Zfp81
Name: zinc finger protein 81
Synonyms: Zfp78, C330034P10Rik, D330034E10Rik, Hszfp36, KRAB13
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224694
Homologene: 138633
Eml2
Name: echinoderm microtubule associated protein like 2
Synonyms: 1600029N02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72205
Homologene: 8125
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: Zfh4, Zfh-4, C130041O22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80892
Homologene: 23477
Wdr87-ps
Name: WD repeat domain 87, pseudogene
Synonyms: 4932431P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114675
Astn2
Name: astrotactin 2
Synonyms: Astnl, 1d8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56079
Homologene: 77850
Man2b1
Name: mannosidase 2, alpha B1
Synonyms: lysosomal alpha-mannosidase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17159
HGNC: HGNC:6826
Homologene: 37322
Rab44
Name: RAB44, member RAS oncogene family
Synonyms: 9830134C10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 442827
Homologene: 53300
Tprg1l
Name: transformation related protein 63 regulated 1 like
Synonyms: Tprgl, mover, 1200015A19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67808
Homologene: 12192
Slx4
Name: SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae)
Synonyms: D16Bwg1016e, Btbd12
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 52864
Homologene: 23770
Myo7b
Name: myosin VIIB
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17922
HGNC: HGNC:7607
Homologene: 81947
Gabrb2
Name: gamma-aminobutyric acid type A receptor subunit beta 2
Synonyms: C030021G16Rik, Gabrb-2, C030002O17Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14401
HGNC: HGNC:4082
Homologene: 7327
Spag16
Name: sperm associated antigen 16
Synonyms: 4921511D23Rik, 4930585K05Rik, 4930524F24Rik, Pf20, Wdr29
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66722
Homologene: 11572
Ttyh2
Name: tweety family member 2
Synonyms: 1110001A03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 117160
Homologene: 41882
Cps1
Name: carbamoyl-phosphate synthetase 1
Synonyms: CPS, 4732433M03Rik, CPSase I, D1Ucla3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227231
HGNC: HGNC:2323
Homologene: 68208
Gli3
Name: GLI-Kruppel family member GLI3
Synonyms: brachyphalangy, Bph
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14634
HGNC: HGNC:4319
Homologene: 139
Lacc1
Name: laccase domain containing 1
Synonyms: 9030625A04Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 210808
Homologene: 17712
Tmf1
Name: TATA element modulatory factor 1
Synonyms: LOC232286, 7030402D04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232286
Homologene: 133801
Fhod3
Name: formin homology 2 domain containing 3
Synonyms: A930009H06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225288
VEGA: 18
Homologene: 45323
Bcas1
Name: brain enriched myelin associated protein 1
Synonyms: breast carcinoma amplified sequence 1, 2210416M21Rik, NABC1, 9030223A09Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76960
HGNC: HGNC:974
Homologene: 2714
Slc23a2
Name: solute carrier family 23 (nucleobase transporters), member 2
Synonyms: SVCT2, Slc23a1, YSPL3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 54338
Homologene: 68440
Serinc2
Name: serine incorporator 2
Synonyms: 2310004K20Rik, Tde2l, TDE2, FKSG84
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230779
Homologene: 27797
Ppp1r3g
Name: protein phosphatase 1, regulatory subunit 3G
Synonyms: 1600032L17Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76487
Homologene: 109865
Aoc3
Name: amine oxidase, copper containing 3
Synonyms: VAP1, semicarbazide-sensitive amine oxidase, SSAO
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11754
HGNC: HGNC:550
Homologene: 2770
Mmp24
Name: matrix metallopeptidase 24
Synonyms: MT5-MMP, Membrane type 5-MMP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17391
HGNC: HGNC:7172
Homologene: 21331
Apobr
Name: apolipoprotein B receptor
Synonyms: Apob-48r, Apob48r
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 171504
Homologene: 26695
Or5p72
Name: olfactory receptor family 5 subfamily P member 72
Synonyms: Olfr497, MOR204-9, GA_x6K02T2PBJ9-10752603-10753547
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258733
Homologene: 133602
Spopl
Name: speckle-type BTB/POZ protein-like
Synonyms: 4921517N04Rik, E430033K04Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76857
Homologene: 78016
Cacnb4
Name: calcium channel, voltage-dependent, beta 4 subunit
Synonyms: Cchb4, 3110038O15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12298
HGNC: HGNC:1404
Homologene: 20188
Cbx8
Name: chromobox 8
Synonyms: polycomb 3, Pc3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 30951
Homologene: 32199
Wdr55
Name: WD repeat domain 55
Synonyms: 2410080P20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67936
VEGA: 18
Homologene: 9789
Tssk5
Name: testis-specific serine kinase 5
Synonyms: 1700091F14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 73542
VEGA: 15
Homologene: 18832
Or13c7b
Name: olfactory receptor family 13 subfamily C member 7B
Synonyms: Olfr156, mOR37b, Olfr37b, MOR262-6, GA_x6K02T2N78B-16125144-16126100, OR37B
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 29846
Homologene: 133652
Gm10420
Name: predicted gene 10420
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108169197
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 67,205,199 bp
  • A to G, chromosome 1 at 70,493,830 bp
  • C to T, chromosome 1 at 75,527,139 bp
  • A to T, chromosome 1 at 177,447,121 bp
  • C to T, chromosome 2 at 23,517,945 bp
  • T to A, chromosome 2 at 52,477,771 bp
  • T to A, chromosome 2 at 79,657,820 bp
  • G to A, chromosome 2 at 132,056,782 bp
  • A to G, chromosome 2 at 155,813,988 bp
  • T to C, chromosome 2 at 170,406,821 bp
  • G to A, chromosome 3 at 5,399,620 bp
  • A to G, chromosome 4 at 43,821,175 bp
  • G to A, chromosome 4 at 43,891,001 bp
  • C to T, chromosome 4 at 46,382,140 bp
  • A to G, chromosome 4 at 66,119,063 bp
  • G to T, chromosome 4 at 130,254,054 bp
  • G to A, chromosome 4 at 154,159,323 bp
  • G to A, chromosome 5 at 124,836,273 bp
  • A to T, chromosome 6 at 97,172,332 bp
  • A to T, chromosome 6 at 122,696,223 bp
  • G to A, chromosome 7 at 19,179,350 bp
  • C to A, chromosome 7 at 29,537,738 bp
  • T to A, chromosome 7 at 108,422,915 bp
  • A to T, chromosome 7 at 126,587,522 bp
  • G to A, chromosome 8 at 85,090,936 bp
  • T to C, chromosome 8 at 111,979,362 bp
  • A to G, chromosome 9 at 7,836,900 bp
  • A to G, chromosome 9 at 8,958,419 bp
  • T to A, chromosome 9 at 108,486,798 bp
  • T to C, chromosome 11 at 42,597,694 bp
  • T to G, chromosome 11 at 101,332,045 bp
  • A to T, chromosome 11 at 114,710,963 bp
  • C to A, chromosome 11 at 119,040,792 bp
  • T to A, chromosome 13 at 15,723,571 bp
  • T to A, chromosome 13 at 35,969,620 bp
  • T to A, chromosome 14 at 77,034,030 bp
  • G to A, chromosome 14 at 103,082,877 bp
  • T to C, chromosome 15 at 76,372,211 bp
  • G to A, chromosome 15 at 81,895,285 bp
  • T to C, chromosome 16 at 3,994,909 bp
  • T to A, chromosome 16 at 15,700,653 bp
  • G to A, chromosome 17 at 29,139,897 bp
  • A to T, chromosome 17 at 33,334,703 bp
  • A to T, chromosome 18 at 6,111,774 bp
  • T to A, chromosome 18 at 25,110,239 bp
  • A to C, chromosome 18 at 31,893,079 bp
  • A to G, chromosome 18 at 32,014,229 bp
  • G to A, chromosome 18 at 36,760,395 bp
  • G to T, chromosome 19 at 18,977,628 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4497 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041750-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.