Strain Name:
C57BL/6J-MtgxR4572Btlr/Mmmh
Stock Number:
041796-MU
Citation ID:
RRID:MMRRC_041796-MU
Other Names:
R4572 (G1), C57BL/6J-MtgxR4572Btlr
Major Collection:

Strain Information

Rgs14
Name: regulator of G-protein signaling 14
Synonyms: Rap1/rap2 interacting protein
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 51791
HGNC: HGNC:9996
Homologene: 4735
Rpa1
Name: replication protein A1
Synonyms: RF-A, 5031405K23Rik, 70kDa, RP-A, Rpa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68275
Homologene: 2208
Zmym1
Name: zinc finger, MYM domain containing 1
Synonyms: 5830412B09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68310
Homologene: 32904
Phf14
Name: PHD finger protein 14
Synonyms: 1110001C23Rik, 5730446A07Rik, 4932409F11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75725
Homologene: 8775
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b1863Clo, b2b553Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: Hsn2, Prkwnk1, 6430573H23Rik, EG406236
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Cnot2
Name: CCR4-NOT transcription complex, subunit 2
Synonyms: 2600016M12Rik, 2810470K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72068
HGNC: HGNC:7878
Homologene: 40953
Trappc9
Name: trafficking protein particle complex 9
Synonyms: 2900005P22Rik, TRS130, 4632408O18Rik, Nibp, 1810044A24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76510
VEGA: 15
Homologene: 81931
Arfgef1
Name: ADP ribosylation factor guanine nucleotide exchange factor 1
Synonyms: BIG1, P200, ARFGEP1, D730028O18Rik, D130059B05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 211673
Homologene: 4687
Ncapd3
Name: non-SMC condensin II complex, subunit D3
Synonyms: 2810487N22Rik, B130055D15Rik, 4632407J06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 78658
VEGA: 9
Homologene: 41021
Kri1
Name: KRI1 homolog
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 215194
Homologene: 133742
Rbck1
Name: RanBP-type and C3HC4-type zinc finger containing 1
Synonyms: Ubce7ip3, HOIL-1L, HOIL-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 24105
Homologene: 32448
Hus1
Name: HUS1 checkpoint clamp component
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15574
HGNC: HGNC:5309
Homologene: 37932
Gabrr3
Name: gamma-aminobutyric acid type A receptor subunit rho 3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 328699
VEGA: 16
Homologene: 26716
Apobec1
Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11810
HGNC: HGNC:604
Homologene: 1243
Rbm39
Name: RNA binding motif protein 39
Synonyms: Caper alpha, 1500012C14Rik, caper, 2310040E03Rik, Rnpc2, B330012G18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 170791
Homologene: 136465
Rab4a
Name: RAB4A, member RAS oncogene family
Synonyms: Rab4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19341
HGNC: HGNC:9781
Homologene: 101538
Ino80
Name: INO80 complex subunit
Synonyms: 2310079N15Rik, INO80, Inoc1, 4632409L19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68142
Homologene: 75070
Zfp296
Name: zinc finger protein 296
Synonyms: 2210018A16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 63872
Homologene: 11185
Terf2ip
Name: telomeric repeat binding factor 2, interacting protein
Synonyms: Rap1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 57321
Homologene: 10357
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: LOC224126, E530005C20Rik, 2210407G14Rik, Hapip
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Wnt9b
Name: wingless-type MMTV integration site family, member 9B
Synonyms: Wnt14b, clf, Wnt15, clf1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22412
Homologene: 2551
Qrfprl
Name: pyroglutamylated RFamide peptide receptor like
Synonyms: C130060K24Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243407
Homologene: 135976
Alox12e
Name: arachidonate lipoxygenase, epidermal
Synonyms: e-LOX1, Alox12-ps2, Alox12-ps1, 8-LOX, Aloxe
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11685
Homologene: 105868
Pigg
Name: phosphatidylinositol glycan anchor biosynthesis, class G
Synonyms: Gpi7
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 433931
Homologene: 39421
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: b2b1203Clo, Dnahc11, avc4, b2b1289Clo, b2b1279Clo, lrd, b2b598Clo, b2b1727Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Ephb2
Name: Eph receptor B2
Synonyms: eteck, Nuk, Cek5, Qek5, Prkm5, Sek3, Erk, Hek5, Drt, Tyro5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13844
HGNC: HGNC:3393
Homologene: 37925
Tmed4
Name: transmembrane p24 trafficking protein 4
Synonyms: 1110014L17Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103694
Homologene: 5308
Mapk15
Name: mitogen-activated protein kinase 15
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 332110
Homologene: 16371
Clcn2
Name: chloride channel, voltage-sensitive 2
Synonyms: nmf240, Clc2, ClC-2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12724
HGNC: HGNC:2020
Homologene: 3213
Gen1
Name: GEN1, Holliday junction 5' flap endonuclease
Synonyms: 5830483C08Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 209334
VEGA: 12
Homologene: 35313
Stxbp5
Name: syntaxin binding protein 5 (tomosyn)
Synonyms: 4930565N16Rik, LGL3, 0710001E20Rik, tomosyn 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 78808
Homologene: 16402
Ulk4
Name: unc-51-like kinase 4
Synonyms: 4932415A06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 209012
Homologene: 41205
Nlrp9b
Name: NLR family, pyrin domain containing 9B
Synonyms: Nalp9b, Nalp-delta
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243874
Homologene: 18530
Duox2
Name: dual oxidase 2
Synonyms: A430065P05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214593
Homologene: 9689
Trim30a
Name: tripartite motif-containing 30A
Synonyms: Trim30, Rpt-1, Rpt1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20128
Homologene: 114426
Gabrb2
Name: gamma-aminobutyric acid type A receptor subunit beta 2
Synonyms: C030021G16Rik, Gabrb-2, C030002O17Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14401
HGNC: HGNC:4082
Homologene: 7327
Ankrd36
Name: ankyrin repeat domain 36
Synonyms: 1700012M14Rik, GC3, 1700008J08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76389
Homologene: 137366
Abca6
Name: ATP-binding cassette, sub-family A member 6
Synonyms: 6330565N06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76184
HGNC: HGNC:36
Homologene: 71264
Ddx60
Name: DExD/H box helicase 60
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234311
Homologene: 23031
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225638
Homologene: 50475
Zfp735
Name: zinc finger protein 735
Synonyms: 1700012C15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76390
Homologene: 88945
Or7e170
Name: olfactory receptor family 7 subfamily E member 170
Synonyms: GA_x6K02T2PVTD-13624132-13623212, Olfr862, MOR146-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258555
Homologene: 115540
Ugt3a2
Name: UDP glycosyltransferases 3 family, polypeptide A2
Synonyms: Ugt3a, Ugt3a1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105887
Homologene: 122787
Spata17
Name: spermatogenesis associated 17
Synonyms: 1700065F16Rik, 4930504I07Rik, 4930513F16Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74717
Homologene: 12551
Slfn1
Name: schlafen 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20555
Homologene: 86967
Cyp17a1
Name: cytochrome P450, family 17, subfamily a, polypeptide 1
Synonyms: Cyp17, p450c17, steroid 17-alpha hydroxylase
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13074
HGNC: HGNC:2593
Homologene: 73875
Cilp2
Name: cartilage intermediate layer protein 2
Synonyms: 1110031K21Rik, CLIP-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68709
Homologene: 17713
Mrpl50
Name: mitochondrial ribosomal protein L50
Synonyms: D4Wsu125e
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 28028
Homologene: 32436
Ptger4
Name: prostaglandin E receptor 4 (subtype EP4)
Synonyms: Ptgerep4, EP4
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19219
HGNC: HGNC:9596
Homologene: 20261
Hmbox1
Name: homeobox containing 1
Synonyms: F830020C16Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219150
Homologene: 11589
Tll1
Name: tolloid-like
Synonyms: Tll-1, b2b2476Clo
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 21892
Homologene: 49202
Npy6r
Name: neuropeptide Y receptor Y6
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 18169
VEGA: 18
HGNC: HGNC:7959
Homologene: 134692
Sesn3
Name: sestrin 3
Synonyms: SEST3, 5630400E15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 75747
Homologene: 14386
Adamtsl2
Name: ADAMTS-like 2
Synonyms: A930008K15Rik, stb
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77794
Homologene: 8798
Mrgpre
Name: MAS-related GPR, member E
Synonyms: MrgE, C130069N09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244238
Homologene: 18469
Trim35
Name: tripartite motif-containing 35
Synonyms: Hls5, A430106H13Rik, 0710005M05Rik, Mair
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 66854
Homologene: 134331
Plppr3
Name: phospholipid phosphatase related 3
Synonyms: Lppr3, BC005764
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216152
Homologene: 11769
Dok6
Name: docking protein 6
Synonyms: Dok-6
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 623279
Homologene: 45143
Epha10
Name: Eph receptor A10
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230735
Homologene: 67804
Fscn3
Name: fascin actin-bundling protein 3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56223
HGNC: HGNC:3961
Homologene: 10475
Mup15
Name: major urinary protein 15
Synonyms: Gm2068
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100039150
Homologene: 74304
Srcin1
Name: SRC kinase signaling inhibitor 1
Synonyms: p140Cap, P140
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56013
Homologene: 10324
Lekr1
Name: leucine, glutamate and lysine rich 1
Synonyms: Gm6534, EG546798
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 624866
Homologene: 54497
Crtap
Name: cartilage associated protein
Synonyms: P3h5, 5730529N23Rik, Leprel3, CASP
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56693
HGNC: HGNC:2379
Homologene: 21280
Gm6866
Name: predicted gene 6866
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 628304
Homologene: 80005
B430119L08Rik
Name: RIKEN cDNA B430119L08 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Gm1993
Name: predicted gene 1993
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 100038977
Homologene: 134350
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 10,213,141 bp
  • T to C, chromosome 1 at 187,193,996 bp
  • A to G, chromosome 2 at 27,083,256 bp
  • G to A, chromosome 2 at 119,402,358 bp
  • T to A, chromosome 2 at 122,281,726 bp
  • G to A, chromosome 2 at 152,318,733 bp
  • A to G, chromosome 2 at 156,177,847 bp
  • A to T, chromosome 3 at 65,783,915 bp
  • A to T, chromosome 4 at 49,514,399 bp
  • A to G, chromosome 4 at 61,438,217 bp
  • A to G, chromosome 4 at 124,902,568 bp
  • A to T, chromosome 4 at 127,050,835 bp
  • A to C, chromosome 4 at 136,655,940 bp
  • A to G, chromosome 5 at 108,332,885 bp
  • G to A, chromosome 6 at 12,006,824 bp
  • T to A, chromosome 6 at 28,430,635 bp
  • A to T, chromosome 6 at 65,454,991 bp
  • C to A, chromosome 6 at 115,955,756 bp
  • T to C, chromosome 6 at 119,951,911 bp
  • T to C, chromosome 6 at 122,581,397 bp
  • A to G, chromosome 7 at 19,584,464 bp
  • G to A, chromosome 7 at 20,026,681 bp
  • G to A, chromosome 7 at 41,216,159 bp
  • G to T, chromosome 7 at 104,411,188 bp
  • G to A, chromosome 7 at 143,781,104 bp
  • A to G, chromosome 8 at 61,987,421 bp
  • A to G, chromosome 8 at 64,056,309 bp
  • A to G, chromosome 8 at 69,882,410 bp
  • A to G, chromosome 8 at 112,012,017 bp
  • A to T, chromosome 8 at 123,834,060 bp
  • G to A, chromosome 9 at 14,321,220 bp
  • A to C, chromosome 9 at 19,883,979 bp
  • A to T, chromosome 9 at 21,280,384 bp
  • A to G, chromosome 9 at 27,094,615 bp
  • T to C, chromosome 9 at 114,384,806 bp
  • T to C, chromosome 9 at 121,192,764 bp
  • T to C, chromosome 10 at 9,838,144 bp
  • T to A, chromosome 10 at 79,866,063 bp
  • T to C, chromosome 10 at 116,494,846 bp
  • A to T, chromosome 11 at 5,689,340 bp
  • CTCTTTCT to CTCT, chromosome 11 at 6,274,461 bp
  • A to T, chromosome 11 at 9,007,617 bp
  • A to G, chromosome 11 at 42,593,917 bp
  • A to G, chromosome 11 at 70,321,181 bp
  • A to G, chromosome 11 at 73,689,785 bp
  • A to G, chromosome 11 at 75,316,236 bp
  • A to T, chromosome 11 at 83,121,463 bp
  • A to C, chromosome 11 at 97,534,934 bp
  • G to A, chromosome 11 at 103,732,155 bp
  • A to T, chromosome 11 at 110,216,548 bp
  • A to G, chromosome 12 at 11,242,418 bp
  • A to T, chromosome 12 at 103,898,708 bp
  • A to T, chromosome 12 at 118,010,125 bp
  • A to T, chromosome 13 at 55,380,062 bp
  • A to G, chromosome 14 at 64,903,233 bp
  • T to C, chromosome 14 at 66,307,873 bp
  • A to C, chromosome 15 at 5,243,133 bp
  • T to A, chromosome 15 at 9,306,393 bp
  • G to T, chromosome 15 at 72,937,067 bp
  • T to C, chromosome 15 at 75,998,750 bp
  • A to G, chromosome 16 at 20,712,139 bp
  • T to C, chromosome 16 at 32,753,802 bp
  • A to T, chromosome 16 at 34,392,042 bp
  • A to T, chromosome 16 at 59,461,638 bp
  • A to T, chromosome 18 at 44,275,917 bp
  • A to T, chromosome 18 at 65,281,004 bp
  • T to A, chromosome 18 at 89,473,947 bp
  • A to G, chromosome 19 at 46,670,551 bp
  • C to T, chromosome X at 25,560,356 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4572 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041796-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.