Strain Name:
C57BL/6J-MtgxR4574Btlr/Mmmh
Stock Number:
041797-MU
Citation ID:
RRID:MMRRC_041797-MU
Other Names:
R4574 (G1), C57BL/6J-MtgxR4574Btlr
Major Collection:

Strain Information

Mthfr
Name: methylenetetrahydrofolate reductase
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17769
HGNC: HGNC:7436
Homologene: 4349
Ddx23
Name: DEAD box helicase 23
Synonyms: 4921506D17Rik, 3110082M05Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 74351
Homologene: 3542
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 9530077A04Rik, Mll5, 1810033J14Rik, D230038D11Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Trpm7
Name: transient receptor potential cation channel, subfamily M, member 7
Synonyms: 2310022G15Rik, 5033407O22Rik, 4833414K03Rik, CHAK1, LTRPC7, CHAK, TRP-PLIK, Ltpr7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58800
Homologene: 9774
Appbp2
Name: amyloid beta precursor protein binding protein 2
Synonyms: 1300003O07Rik, PAT1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66884
HGNC: HGNC:622
Homologene: 31378
Ccdc93
Name: coiled-coil domain containing 93
Synonyms: 9230102M16Rik, 4633402D15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70829
Homologene: 10393
Anapc1
Name: anaphase promoting complex subunit 1
Synonyms: tsg24, 2610021O03Rik, Apc1, Mcpr
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17222
Homologene: 7414
Pspc1
Name: paraspeckle protein 1
Synonyms: 5730470C09Rik, PSP1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 66645
Homologene: 10108
Dlx6
Name: distal-less homeobox 6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13396
HGNC: HGNC:2919
Homologene: 87855
Ubtf
Name: upstream binding transcription factor, RNA polymerase I
Synonyms: A930005G04Rik, Tcfubf, UBF, UBF1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21429
Homologene: 7970
Pikfyve
Name: phosphoinositide kinase, FYVE type zinc finger containing
Synonyms: Pip5k3, PipkIII, 5230400C17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18711
Homologene: 32115
Sumf1
Name: sulfatase modifying factor 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 58911
Homologene: 16268
Tjp1
Name: tight junction protein 1
Synonyms: ZO-1, ZO1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21872
Homologene: 2445
Zfp592
Name: zinc finger protein 592
Synonyms: A730014M16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233410
Homologene: 8759
Ms4a14
Name: membrane-spanning 4-domains, subfamily A, member 14
Synonyms: LOC383435
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 383435
Homologene: 138453
Cdk12
Name: cyclin dependent kinase 12
Synonyms: Crk7, 1810022J16Rik, D11Ertd752e, Crkrs
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69131
Homologene: 128632
Nup54
Name: nucleoporin 54
Synonyms: 3110079L04Rik, 54kDa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269113
Homologene: 41169
Mpp7
Name: membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7)
Synonyms: 5430426E14Rik, LOC381166, 2810038M04Rik, 1110068J02Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 75739
VEGA: 18
Homologene: 1455
Sez6l
Name: seizure related 6 homolog like
Synonyms: Acig1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56747
Homologene: 10895
Steap3
Name: STEAP family member 3
Synonyms: 1010001D01Rik, pHyde
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68428
Homologene: 10084
Rorc
Name: RAR-related orphan receptor gamma
Synonyms: thymus orphan receptor, Thor, RORgamma
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19885
Homologene: 21051
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: Dnahc5, Mdnah5, b2b1134Clo, b2b1154Clo, b2b1537Clo, b2b1565Clo, b2b3491Clo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: Dnahc6, A730004I20Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Rusc2
Name: RUN and SH3 domain containing 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100213
Homologene: 18967
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: b2b1203Clo, Dnahc11, avc4, b2b1289Clo, b2b1279Clo, lrd, b2b598Clo, b2b1727Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Ralgapa2
Name: Ral GTPase activating protein, alpha subunit 2 (catalytic)
Synonyms: RGC2, A230067G21Rik, AS250
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241694
Homologene: 28131
Pnldc1
Name: poly(A)-specific ribonuclease (PARN)-like domain containing 1
Synonyms: LOC240023
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240023
VEGA: 17
Homologene: 15054
Carmil3
Name: capping protein regulator and myosin 1 linker 3
Synonyms: Lrrc16b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268747
VEGA: 14
Homologene: 74564
Vmn2r19
Name: vomeronasal 2, receptor 19
Synonyms: EG232358
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232358
Homologene: 84037
Gsdmc2
Name: gasdermin C2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 331063
HGNC: HGNC:7151
Homologene: 69487
Adamts9
Name: ADAM metallopeptidase with thrombospondin type 1 motif 9
Synonyms: 1810011L16Rik, UND3, Mhdaund4, Gsfund3, 8430403M15Rik, Mhdaund3, E030027K14Rik, UND4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101401
Homologene: 18821
Cd101
Name: CD101 antigen
Synonyms: Igsf2, LOC381460
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 630146
HGNC: HGNC:5949
Homologene: 3142
Pex3
Name: peroxisomal biogenesis factor 3
Synonyms: 1700014F15Rik, 2810027F19Rik, 2900010N04Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 56535
HGNC: HGNC:8858
Homologene: 2691
Sspo
Name: SCO-spondin
Synonyms: Scospondin, C79529
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243369
Homologene: 45453
Plcl2
Name: phospholipase C-like 2
Synonyms: Plce2, PRIP-2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224860
VEGA: 17
HGNC: HGNC:9064
Homologene: 9052
Ofcc1
Name: orofacial cleft 1 candidate 1
Synonyms: Opo, ojoplano
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218165
VEGA: 13
Homologene: 125376
Tsfm
Name: Ts translation elongation factor, mitochondrial
Synonyms: EF-TS, EF-Tsmt, 2310050B20Rik, 9430024O13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66399
Homologene: 4184
Myo5c
Name: myosin VC
Synonyms: 9130003O20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 208943
VEGA: 9
HGNC: HGNC:7604
Homologene: 135711
Or5ak22
Name: olfactory receptor family 5 subfamily AK member 22
Synonyms: MOR203-1, GA_x6K02T2Q125-46877170-46876241, Olfr992
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258865
Homologene: 121517
Fpr-rs6
Name: formyl peptide receptor, related sequence 6
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 321020
HGNC: HGNC:3827
Homologene: 104303
Clcn4
Name: chloride channel, voltage-sensitive 4
Synonyms: Clcn4-2, Clc4-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12727
HGNC: HGNC:2022
Homologene: 68207
Ctf2
Name: cardiotrophin 2
Synonyms: NP, neuropoietin, LOC244218
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244218
Homologene: 19463
Cpt1b
Name: carnitine palmitoyltransferase 1b, muscle
Synonyms: M-CPTI, Cpt1, M-CPT I
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12895
HGNC: HGNC:2329
Homologene: 22548
Gm4841
Name: predicted gene 4841
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225594
VEGA: 18
Slc22a14
Name: solute carrier family 22 (organic cation transporter), member 14
Synonyms: LOC382113
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382113
HGNC: HGNC:8495
Homologene: 3530
Pom121l2
Name: POM121 transmembrane nucleoporin like 2
Synonyms: LOC195236
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 195236
Homologene: 123536
Maip1
Name: matrix AAA peptidase interacting protein 1
Synonyms: 9430016H08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68115
Homologene: 11565
Pccb
Name: propionyl Coenzyme A carboxylase, beta polypeptide
Synonyms: 1300012P06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66904
HGNC: HGNC:8654
Homologene: 447
Rsph3b
Name: radial spoke 3B homolog (Chlamydomonas)
Synonyms: Rshl2b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 100037282
VEGA: 17
Homologene: 12043
Neurl1b
Name: neuralized E3 ubiquitin protein ligase 1B
Synonyms: EG240055, C230078M08Rik, Neur2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240055
Homologene: 35443
Telo2
Name: telomere maintenance 2
Synonyms: 1200003M09Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 71718
Homologene: 41107
Irx5
Name: Iroquois homeobox 5
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54352
Homologene: 38105
Pate2
Name: prostate and testis expressed 2
Synonyms: LOC330921, mANLP1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330921
Homologene: 86016
Upk3a
Name: uroplakin 3A
Synonyms: 1110017C07Rik, Upk3
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 22270
VEGA: 15
Homologene: 5066
Dmrtb1
Name: DMRT-like family B with proline-rich C-terminal, 1
Synonyms: Prp13, Dmrt6
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56296
Homologene: 13207
Mtres1
Name: mitochondrial transcription rescue factor 1
Synonyms: 1700021F05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67851
Homologene: 41137
Adam1b
Name: a disintegrin and metallopeptidase domain 1b
Synonyms: PH-30 alpha, Ftna, fertilin alpha
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 280667
Homologene: 136485
Rpl3l
Name: ribosomal protein L3-like
Synonyms: 1110057H16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66211
Homologene: 68434
0610039K10Rik
Name: RIKEN cDNA 0610039K10 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68386
Bcas2
Name: BCAS2 pre-mRNA processing factor
Synonyms: C76366, breast carcinoma amplified sequence 2, 6430539P16Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68183
HGNC: HGNC:975
Homologene: 4291
Cltb
Name: clathrin light chain B
Synonyms: 2310046E19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 74325
HGNC: HGNC:2091
Homologene: 37532
Rgs13
Name: regulator of G-protein signaling 13
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 246709
HGNC: HGNC:9995
Homologene: 14774
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 57,413,245 bp
  • T to A, chromosome 1 at 65,192,192 bp
  • G to T, chromosome 1 at 120,241,456 bp
  • T to A, chromosome 1 at 121,496,999 bp
  • T to A, chromosome 1 at 144,140,845 bp
  • C to A, chromosome 2 at 85,400,026 bp
  • A to T, chromosome 2 at 126,797,211 bp
  • G to T, chromosome 2 at 128,627,195 bp
  • A to G, chromosome 2 at 146,435,999 bp
  • A to G, chromosome 2 at 163,645,735 bp
  • T to C, chromosome 3 at 94,388,984 bp
  • T to C, chromosome 3 at 101,013,153 bp
  • C to T, chromosome 3 at 103,174,350 bp
  • A to G, chromosome 4 at 43,416,080 bp
  • A to T, chromosome 4 at 107,677,068 bp
  • A to G, chromosome 4 at 148,043,541 bp
  • T to A, chromosome 5 at 23,492,407 bp
  • T to A, chromosome 5 at 92,425,782 bp
  • T to C, chromosome 5 at 112,428,478 bp
  • A to C, chromosome 5 at 121,500,793 bp
  • G to T, chromosome 6 at 6,865,305 bp
  • G to A, chromosome 6 at 48,465,523 bp
  • T to A, chromosome 6 at 73,086,181 bp
  • G to A, chromosome 6 at 92,879,959 bp
  • A to G, chromosome 6 at 108,108,432 bp
  • C to T, chromosome 6 at 123,315,980 bp
  • T to C, chromosome 7 at 7,287,805 bp
  • A to G, chromosome 7 at 65,322,605 bp
  • A to G, chromosome 7 at 81,023,786 bp
  • T to G, chromosome 7 at 127,719,384 bp
  • G to A, chromosome 8 at 92,358,262 bp
  • C to A, chromosome 9 at 35,685,673 bp
  • T to A, chromosome 9 at 67,951,683 bp
  • A to G, chromosome 9 at 75,269,611 bp
  • T to C, chromosome 9 at 100,985,199 bp
  • A to G, chromosome 9 at 119,179,495 bp
  • G to A, chromosome 10 at 13,535,571 bp
  • T to A, chromosome 10 at 43,533,010 bp
  • A to C, chromosome 10 at 127,028,373 bp
  • A to T, chromosome 11 at 85,209,938 bp
  • A to G, chromosome 11 at 98,220,988 bp
  • T to C, chromosome 11 at 102,306,765 bp
  • A to G, chromosome 12 at 118,012,255 bp
  • T to A, chromosome 13 at 21,984,402 bp
  • G to A, chromosome 13 at 40,015,388 bp
  • C to T, chromosome 13 at 54,598,761 bp
  • GGACGA to GGA, chromosome 14 at 55,499,476 bp
  • A to T, chromosome 14 at 56,761,947 bp
  • C to T, chromosome 15 at 28,367,763 bp
  • C to T, chromosome 15 at 63,828,023 bp
  • G to T, chromosome 15 at 85,020,551 bp
  • G to T, chromosome 15 at 89,424,044 bp
  • G to A, chromosome 15 at 98,647,624 bp
  • A to G, chromosome 17 at 6,905,039 bp
  • T to C, chromosome 17 at 12,892,782 bp
  • T to A, chromosome 17 at 20,183,097 bp
  • A to C, chromosome 17 at 24,734,010 bp
  • T to C, chromosome 17 at 25,101,673 bp
  • A to G, chromosome 17 at 26,431,886 bp
  • T to C, chromosome 17 at 50,607,846 bp
  • G to T, chromosome 18 at 7,353,228 bp
  • T to C, chromosome 18 at 60,269,926 bp
  • T to C, chromosome 19 at 11,303,971 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4574 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041797-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.