Strain Name:
B6.129S7-Zpbp2tm1Zuk/Mmjax
Stock Number:
042297-JAX
Citation ID:
RRID:MMRRC_042297-JAX
Other Names:
B6.Zpbp2-/-, Zpbp2 KO

Strain Information

Zpbp2
Name: zona pellucida binding protein 2
Synonyms: 1700017D11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69376
Homologene: 18892
Genetic Alterations
Zpbp2 (Zona pellucida binding protein) knock-out mice exhibit male infertility, altered lipid metabolism, decreased levels of docosahexanoic acid (DHA), increased body weight and a decreased sensitivity to allergic asthma. This targeted mutation deletes exons 1-3, removing part of the promoter/enhancer region and affecting expression of Zpbp2 and Ormdl3 .

Genotype Determination
ES Cell Line
AB2.2
Phenotype
Zona pellucida binding protein 2 or Zpbp2 is a sulfated glycoprotein found in spermatid and spermatozoon and localized to the acrosome. It is involved in binding to the oocyte zona pellucida. In addition, Zpbp2 is located in a conserved 200 kb asthma-associated locus. The Zpbp2 promoter overlaps with an enhancer region affecting expression of Ormdl3 (ORM1-like 3) a sphingolipid biosynthesis regulator. ORMDL3 is associated with endoplasmic reticulum stress and smooth muscle contraction. This targeted mutation deletes exons 1-3, removing part of the promoter/enhancer region affecting expression of both genes.

Female mice that are homozygous for the targeted knock-out (KO) mutation are viable and fertile. Homozygous males are subfertile and average 3 pups per litter. Males on a mixed C57BL/6 and 129S7 background exhibit abnormal sperm morphology and a reduced ability of sperm to penetrate the zona pelucida. Sperm morphology has not been examined on the congenic background. Both heterozygote and homozygote KO mice exhibit a gain in DNA methylation at the promoter/enhancer region. In addition to reduced fertility, homozygotes exhibit altered lipid metabolism, decreased levels of docosahexanoic acid (DHA), and increased body weight. Sensitization with ovalbumin results in reduced bronchial reactivity. This strain may be useful for studying male infertility, the regulation of airway hypersensitivity and lipid metabolism.
Strain Development
A targeting vector containing a PgkHPRT cassette was used to disrupt exons 1, 2 and 3, which encode the signal peptide region. The construct was electroporated into 129S7/SvEvBrd-Hprt1b-m2 derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J female mice and offspring were backcrossed to C57BL/6J for 10 generations.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
  • Obesity
  • Reproduction
Donor
Anna Naumova, Ph.D., The Research Institute of the McGill University Health Centre.
Primary Reference

Kanagaratham C, Chiwara V, Ho B, Moussette S, Youssef M, Venuto D, JeannotteL, Bourque G, de Sanctis JB, Radzioch D, Naumova AK. Loss of the zonapellucida-binding protein 2 (Zpbp2) gene in mice impacts airway hypersensitivity and lung lipid metabolism in a sex-dependent fashion. Mamm Genome. 2018Apr;29(3-4):281-298. doi: 10.1007/s00335-018-9743-x. Epub 2018 Mar 13. (Medline PMID: 29536159)

Colony and Husbandry Information

Females are very sensitive to stress within the first few days after birth and will abandon babies if disturbed.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
black
MMRRC Breeding System
Sib-mating
Generation
10
Overall Breeding Performance
Poor
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Reduced
Heterozygotes are fertile: N/A N/A
Age Reproductive Decline: 6 to 8 months Undetermined
Average litter size
Less than 4
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042297-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
042297-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.