Strain Name:
C57BL/6J-MtgxR4957Btlr/Mmmh
Stock Number:
042554-MU
Citation ID:
RRID:MMRRC_042554-MU
Other Names:
R4957 (G1), C57BL/6J-MtgxR4957Btlr
Major Collection:

Strain Information

Cd74
Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
Synonyms: CLIP, Ii
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16149
VEGA: 18
HGNC: HGNC:1697
Homologene: 3209
Satb1
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20230
Homologene: 2232
Epha7
Name: Eph receptor A7
Synonyms: MDK1, Hek11, Mdk1, Cek11, Ebk, Ehk3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13841
HGNC: HGNC:3390
Homologene: 20935
Ptpn14
Name: protein tyrosine phosphatase, non-receptor type 14
Synonyms: PTP36, OTTMUSG00000022087, C130080N23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19250
HGNC: HGNC:9647
Homologene: 3941
Frrs1
Name: ferric-chelate reductase 1
Synonyms: Sdfr2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20321
Homologene: 40653
Hdac5
Name: histone deacetylase 5
Synonyms: mHDA1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15184
Homologene: 3995
Tlk2
Name: tousled-like kinase 2 (Arabidopsis)
Synonyms: 4933403M19Rik, PKUalpha, protein kinase U-alpha
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 24086
Homologene: 4993
Usp5
Name: ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms: Ucht
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22225
Homologene: 55758
Ncaph
Name: non-SMC condensin I complex, subunit H
Synonyms: A730011O11Rik, Brrn1, HCAP-H
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 215387
HGNC: HGNC:1112
Homologene: 133986
Mtdh
Name: metadherin
Synonyms: Lyric, 3D3/Lyric, AEG-1, 2610103J23Rik, D8Bwg1112e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 67154
Homologene: 12089
Tcf7l2
Name: transcription factor 7 like 2, T cell specific, HMG box
Synonyms: mTcf-4E, TCF4E, TCF4B, mTcf-4B, Tcf-4, Tcf4
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21416
Homologene: 7564
Atf7ip
Name: activating transcription factor 7 interacting protein
Synonyms: Mcaf1, ATFa-associated Modulator, AM, 2610204M12Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 54343
Homologene: 10051
Srsf10
Name: serine and arginine-rich splicing factor 10
Synonyms: TASR1, FUSIP2, SRrp40, NSSR1, NSSR2, Sfrs13a, Srsf13a, TASR2, Nssr, Fusip1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14105
Homologene: 134051
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: 9930018I23Rik, MAP1C, Swl, Dnec1, Loa, Dnchc1, dynein heavy chain, retrograde transport
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Tdrd3
Name: tudor domain containing 3
Synonyms: 4732418C03Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219249
Homologene: 12771
Skic3
Name: SKI3 subunit of superkiller complex
Synonyms: Ttc37
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218343
VEGA: 13
Homologene: 40966
Clptm1l
Name: CLPTM1-like
Synonyms: C130052I12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218335
VEGA: 13
Homologene: 12767
Mnat1
Name: menage a trois 1
Synonyms: E130115E11Rik, MAT1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 17420
HGNC: HGNC:7181
Homologene: 1821
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Grm7
Name: glutamate receptor, metabotropic 7
Synonyms: Gpr1g, E130018M02Rik, mGluR7, mGlu7a receptor, 6330570A01Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108073
HGNC: HGNC:4599
Homologene: 20233
Crebbp
Name: CREB binding protein
Synonyms: KAT3A, CBP
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12914
HGNC: HGNC:2348
Homologene: 68393
Ybx1
Name: Y box protein 1
Synonyms: YB-1, DNA binding protein B, EF1A, dbpB, Nsep1, MSY1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22608
HGNC: HGNC:8014
Homologene: 88707
Slc39a14
Name: solute carrier family 39 (zinc transporter), member 14
Synonyms: G630015O18Rik, Zip14
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 213053
Homologene: 15037
Gemin2
Name: gem nuclear organelle associated protein 2
Synonyms: Sip1, 1700012N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66603
Homologene: 37827
Tnc
Name: tenascin C
Synonyms: C130033P17Rik, TN, hexabrachion, Hxb, cytotactin, tenascin-C, TN-C
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21923
HGNC: HGNC:5318
Homologene: 55636
Arhgap33
Name: Rho GTPase activating protein 33
Synonyms: Tcgap, Snx26, NOMA-GAP
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233071
Homologene: 76448
Mme
Name: membrane metallo endopeptidase
Synonyms: neutral endopeptidase, NEP, neprilysin, 6030454K05Rik, CD10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17380
HGNC: HGNC:7154
Homologene: 5275
Cdc23
Name: CDC23 cell division cycle 23
Synonyms: D18Ertd243e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 52563
HGNC: HGNC:1724
Homologene: 3426
Yme1l1
Name: YME1-like 1 (S. cerevisiae)
Synonyms: ATP-dependent metalloprotease FtsH1, Ftsh
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27377
Homologene: 31996
Prlr
Name: prolactin receptor
Synonyms: Pr-1, Prlr-rs1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19116
HGNC: HGNC:9446
Homologene: 733
Maml2
Name: mastermind like transcriptional coactivator 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270118
Homologene: 134147
Itga11
Name: integrin alpha 11
Synonyms: 4732459H24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319480
HGNC: HGNC:6136
Homologene: 8151
Lmod2
Name: leiomodin 2 (cardiac)
Synonyms: C-Lmod
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 93677
HGNC: HGNC:6648
Homologene: 28302
Slc9c1
Name: solute carrier family 9, subfamily C (Na+-transporting carboxylic acid decarboxylase), member 1
Synonyms: LOC208169, spermNHE, Slc9a10
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208169
Homologene: 19505
Dnah17
Name: dynein, axonemal, heavy chain 17
Synonyms: LOC382552, Dnahcl1, Dnahc17, 2810003K23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69926
HGNC: HGNC:2946
Homologene: 72102
Tnfrsf1b
Name: tumor necrosis factor receptor superfamily, member 1b
Synonyms: TNFBR, p75 TNFR, TNFalpha-R2, TNF-R2, TNFRII, p75, TNF-alphaR2, Tnfr2, TNF-R75, TNF-R-II, TNFR80, CD120b
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21938
Homologene: 829
3110009E18Rik
Name: RIKEN cDNA 3110009E18 gene
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73103
Homologene: 41754
Pcdhb13
Name: protocadherin beta 13
Synonyms: Pcdbh6, PcdhbM
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93884
HGNC: HGNC:8691
Homologene: 10338
Ccdc150
Name: coiled-coil domain containing 150
Synonyms: 4930511H11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78016
Homologene: 15814
Scarf1
Name: scavenger receptor class F, member 1
Synonyms: SREC, SREC-I
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380713
Homologene: 2741
Frmpd1
Name: FERM and PDZ domain containing 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 666060
Homologene: 8939
Pnliprp2
Name: pancreatic lipase-related protein 2
Synonyms: PLRP2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18947
VEGA: 19
HGNC: HGNC:9157
Homologene: 3936
Gmcl1
Name: germ cell-less, spermatogenesis associated 1
Synonyms: 2810049L19Rik, Gcl, mglc-1, Btbd13
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 23885
Homologene: 8021
Ift22
Name: intraflagellar transport 22
Synonyms: Rabl5, 3110017O03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67286
Homologene: 11253
Zfp422
Name: zinc finger protein 422
Synonyms: KOX15, 2900028O21Rik, Krox-26, Krox25, Krox-25-2, Krox26
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67255
Homologene: 5067
Tssk4
Name: testis-specific serine kinase 4
Synonyms: 4933424F08Rik, 1700020B19Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71099
VEGA: 14
Homologene: 57078
Tcam1
Name: testicular cell adhesion molecule 1
Synonyms: 4930570F09Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75870
Homologene: 123944
Or5m9b
Name: olfactory receptor family 5 subfamily M member 9B
Synonyms: MOR262-13, GA_x6K02T2Q125-47549689-47550621, Olfr1036, MOR245-25
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258245
H2-T10
Name: histocompatibility 2, T region locus 10
Synonyms: H-2T10
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15024
Ercc3
Name: excision repair cross-complementing rodent repair deficiency, complementation group 3
Synonyms: XPB
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13872
HGNC: HGNC:3435
Homologene: 96
Vmn2r8
Name: vomeronasal 2, receptor 8
Synonyms: EG627479
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 627479
Homologene: 129606
Scart1
Name: scavenger receptor family member expressed on T cells 1
Synonyms: E430002D04Rik, Cd163l1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244233
Homologene: 129778
Creb5
Name: cAMP responsive element binding protein 5
Synonyms: 9430076C15Rik, D430026C09Rik, Crebpa
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 231991
Homologene: 18215
6820408C15Rik
Name: RIKEN cDNA 6820408C15 gene
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228778
Homologene: 51916
Cdc25b
Name: cell division cycle 25B
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12531
HGNC: HGNC:1726
Homologene: 41451
Ugt3a1
Name: UDP glycosyltransferases 3 family, polypeptide A1
Synonyms: Ugt3a2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223337
Homologene: 122787
Pla2g4f
Name: phospholipase A2, group IVF
Synonyms: 4732472I07Rik, Pla2zeta
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 271844
Homologene: 77933
Ighg3
Name: Immunoglobulin heavy constant gamma 3
Synonyms: AI324046, IgG3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 380795
Zpbp2
Name: zona pellucida binding protein 2
Synonyms: 1700017D11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69376
Homologene: 18892
Cacnb4
Name: calcium channel, voltage-dependent, beta 4 subunit
Synonyms: Cchb4, 3110038O15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12298
HGNC: HGNC:1404
Homologene: 20188
Fbxl8
Name: F-box and leucine-rich repeat protein 8
Synonyms: FBL8
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50788
Homologene: 9279
Enkd1
Name: enkurin domain containing 1
Synonyms: E130303B06Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102124
Homologene: 12957
Elovl1
Name: ELOVL fatty acid elongase 1
Synonyms: elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 1, Ssc1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 54325
Homologene: 41322
Glra1
Name: glycine receptor, alpha 1 subunit
Synonyms: B230397M16Rik, oscillator, nmf11, ot
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14654
HGNC: HGNC:4326
Homologene: 20083
Or9g8
Name: olfactory receptor family 9 subfamily G member 8
Synonyms: MOR213-5, Olfr1014, GA_x6K02T2Q125-47255337-47256254
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258562
Homologene: 83447
Gpr15
Name: G protein-coupled receptor 15
Synonyms: 4933439K08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 71223
VEGA: 16
HGNC: HGNC:4469
Homologene: 3869
Or2t43
Name: olfactory receptor family 2 subfamily T member 43
Synonyms: Olfr327-ps1, GA_x6K02T00261-652-347, GA_x6K02T2NKPP-858022-858862, MOR275-10_p, Olfr224, MOR275-3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258198
Homologene: 133015
Or4f17-ps1
Name: olfactory receptor family 4 subfamily F member 17, pseudogene 1
Synonyms: GA_x6K02T2Q125-72578807-72579745, MOR245-24P, OTTMUSG00000015076, Olfr1293-ps
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 623583
Gm12256
Name: predicted gene 12256
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 54,364,868 bp
  • G to C, chromosome 1 at 120,169,110 bp
  • G to T, chromosome 1 at 120,169,119 bp
  • C to T, chromosome 1 at 120,169,120 bp
  • C to T, chromosome 1 at 189,851,272 bp
  • G to A, chromosome 2 at 23,193,224 bp
  • T to C, chromosome 2 at 52,558,291 bp
  • T to C, chromosome 2 at 85,777,115 bp
  • T to A, chromosome 2 at 86,075,510 bp
  • C to A, chromosome 2 at 111,528,224 bp
  • C to T, chromosome 2 at 120,300,499 bp
  • T to C, chromosome 2 at 127,121,257 bp
  • T to C, chromosome 2 at 131,193,605 bp
  • T to A, chromosome 2 at 152,444,093 bp
  • T to A, chromosome 3 at 63,343,489 bp
  • G to A, chromosome 3 at 116,885,248 bp
  • C to T, chromosome 4 at 28,871,892 bp
  • T to G, chromosome 4 at 45,273,099 bp
  • G to C, chromosome 4 at 63,976,556 bp
  • A to T, chromosome 4 at 118,431,923 bp
  • A to T, chromosome 4 at 119,278,938 bp
  • T to C, chromosome 4 at 135,856,230 bp
  • C to A, chromosome 4 at 145,246,757 bp
  • T to G, chromosome 4 at 145,246,758 bp
  • T to G, chromosome 5 at 108,799,263 bp
  • G to A, chromosome 5 at 136,908,216 bp
  • T to C, chromosome 6 at 24,603,872 bp
  • A to T, chromosome 6 at 53,693,922 bp
  • G to A, chromosome 6 at 86,710,521 bp
  • G to A, chromosome 6 at 111,358,863 bp
  • T to A, chromosome 6 at 116,626,943 bp
  • C to G, chromosome 6 at 124,822,630 bp
  • C to T, chromosome 6 at 136,606,810 bp
  • C to T, chromosome 7 at 30,532,361 bp
  • T to C, chromosome 7 at 140,228,522 bp
  • A to G, chromosome 8 at 105,268,195 bp
  • A to G, chromosome 8 at 105,704,489 bp
  • A to G, chromosome 9 at 13,620,276 bp
  • A to T, chromosome 9 at 62,767,648 bp
  • T to A, chromosome 11 at 55,527,398 bp
  • A to G, chromosome 11 at 58,566,518 bp
  • T to A, chromosome 11 at 58,891,231 bp
  • A to G, chromosome 11 at 75,525,634 bp
  • T to A, chromosome 11 at 98,551,324 bp
  • C to A, chromosome 11 at 102,205,256 bp
  • T to C, chromosome 11 at 105,253,359 bp
  • T to A, chromosome 11 at 106,282,879 bp
  • A to T, chromosome 11 at 118,074,298 bp
  • A to G, chromosome 12 at 59,017,168 bp
  • A to G, chromosome 12 at 73,123,878 bp
  • C to A, chromosome 12 at 110,658,126 bp
  • T to C, chromosome 12 at 113,361,130 bp
  • T to C, chromosome 13 at 11,785,080 bp
  • T to A, chromosome 13 at 73,611,196 bp
  • T to C, chromosome 13 at 73,612,428 bp
  • T to G, chromosome 13 at 76,185,113 bp
  • A to T, chromosome 14 at 55,651,809 bp
  • A to T, chromosome 14 at 70,315,811 bp
  • T to A, chromosome 14 at 87,505,787 bp
  • G to A, chromosome 15 at 9,365,188 bp
  • G to T, chromosome 15 at 10,319,195 bp
  • A to T, chromosome 15 at 34,083,135 bp
  • T to C, chromosome 16 at 4,117,367 bp
  • A to T, chromosome 16 at 45,544,831 bp
  • G to T, chromosome 16 at 58,718,174 bp
  • T to A, chromosome 17 at 36,117,416 bp
  • A to G, chromosome 17 at 51,803,548 bp
  • T to A, chromosome 18 at 32,243,116 bp
  • G to T, chromosome 18 at 32,243,117 bp
  • C to T, chromosome 18 at 34,637,343 bp
  • T to A, chromosome 18 at 37,444,784 bp
  • A to G, chromosome 18 at 60,809,037 bp
  • A to G, chromosome 19 at 55,931,432 bp
  • C to T, chromosome 19 at 58,775,145 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4957 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042554-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.