Strain Name:
C57BL/6J-MtgxR4975Btlr/Mmmh
Stock Number:
042570-MU
Citation ID:
RRID:MMRRC_042570-MU
Other Names:
R4975 (G1), C57BL/6J-MtgxR4975Btlr
Major Collection:

Strain Information

Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, MYH-beta/slow, beta-MHC, B-MHC, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Snx1
Name: sorting nexin 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56440
Homologene: 99716
Il21
Name: interleukin 21
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 60505
HGNC: HGNC:6005
Homologene: 11032
Msi2
Name: musashi RNA-binding protein 2
Synonyms: msi2h, Musashi2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76626
Homologene: 62199
Abcc8
Name: ATP-binding cassette, sub-family C member 8
Synonyms: SUR1, Sur, D930031B21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20927
HGNC: HGNC:59
Homologene: 68048
Kansl1
Name: KAT8 regulatory NSL complex subunit 1
Synonyms: 1700081L11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76719
Homologene: 9140
Setx
Name: senataxin
Synonyms: A930037J23Rik, Als4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269254
HGNC: HGNC:445
Homologene: 41003
Srrm1
Name: serine/arginine repetitive matrix 1
Synonyms: SRm160
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 51796
Clasp2
Name: CLIP associating protein 2
Synonyms: 1500004F14Rik, CLASP2gamma, CLASP2beta, CLASP2alpha, CLASP2, 8030404L10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76499
VEGA: 9
Homologene: 24944
Ralgapb
Name: Ral GTPase activating protein, beta subunit (non-catalytic)
Synonyms: B230339M05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228850
Homologene: 10666
Hectd1
Name: HECT domain E3 ubiquitin protein ligase 1
Synonyms: A630086P08Rik, opm, b2b327Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 207304
VEGA: 12
Homologene: 9115
Zfp619
Name: zinc finger protein 619
Synonyms: 3000002G13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70227
Homologene: 133818
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: E130014J05Rik, Ayu17-449
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214133
Homologene: 49498
Gtf2ird1
Name: general transcription factor II I repeat domain-containing 1
Synonyms: WBSCR11, Cream1, GTF3, MusTRD1, binding factor for early enhancer, BEN, ESTM9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 57080
HGNC: HGNC:4661
Homologene: 4158
Rapgef1
Name: Rap guanine nucleotide exchange factor (GEF) 1
Synonyms: C3G, 4932418O06Rik, Grf2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 107746
HGNC: HGNC:4568
Homologene: 50501
Rttn
Name: rotatin
Synonyms: 4921538A15Rik, C530033I08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 246102
VEGA: 18
Homologene: 65275
Nol6
Name: nucleolar protein family 6 (RNA-associated)
Synonyms: Nrap
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230082
Homologene: 41505
Stk39
Name: serine/threonine kinase 39
Synonyms: DCHT, SPAK, RF005, Rnl5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 53416
Homologene: 22739
Dmap1
Name: DNA methyltransferase 1-associated protein 1
Synonyms: 1500016M21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66233
Homologene: 41311
Ccni
Name: cyclin I
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12453
HGNC: HGNC:1595
Homologene: 4979
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 209018
Homologene: 44592
Cpsf2
Name: cleavage and polyadenylation specific factor 2
Synonyms: Cpsf, 2610024B04Rik, 100kDa
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 51786
VEGA: 12
HGNC: HGNC:2325
Homologene: 6460
Mia3
Name: MIA SH3 domain ER export factor 3
Synonyms: LOC385255, Tango, A930039G15Rik, B230399H06Rik, 9130229H14Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 338366
Homologene: 85165
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Twf2
Name: twinfilin actin binding protein 2
Synonyms: A6-related, Twf2, Twinfilin-2, Ptk9l
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23999
HGNC: HGNC:9621
Homologene: 5272
Svil
Name: supervillin
Synonyms: B430302E16Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225115
Homologene: 25090
Siglece
Name: sialic acid binding Ig-like lectin E
Synonyms: mSiglec-E, Siglecl1, Siglec5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 83382
Homologene: 130669
Lmo2
Name: LIM domain only 2
Synonyms: Rbtn-2, Rbtn2, Rhom-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16909
HGNC: HGNC:6642
Homologene: 4072
Hmcn2
Name: hemicentin 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 665700
Homologene: 90772
Reln
Name: reelin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Or52h1
Name: olfactory receptor family 52 subfamily H member 1
Synonyms: GA_x6K02T2PBJ9-6914780-6913830, MOR31-12, Olfr648
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258746
Homologene: 82999
Zfp608
Name: zinc finger protein 608
Synonyms: 4932417D18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 269023
VEGA: 18
Homologene: 18485
Or5d18
Name: olfactory receptor family 5 subfamily D member 18
Synonyms: mOR-EG, MOR174-9, GA_x6K02T2Q125-49527073-49526132, Olfr73
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 117004
Homologene: 133889
Rab11fip4
Name: RAB11 family interacting protein 4 (class II)
Synonyms: RAB11-FIP4, A730072L08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268451
Homologene: 8803
Sybu
Name: syntabulin (syntaxin-interacting)
Synonyms: A830027B17Rik, Golsyn/Syntabulin, 5730410E15Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 319613
VEGA: 15
Homologene: 9838
Slco1a8
Name: solute carrier organic anion transporter family, member 1a8
Synonyms: Gm6614
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 625716
Homologene: 87075
Tfip11
Name: tuftelin interacting protein 11
Synonyms: Tip39
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 54723
Homologene: 40813
Xylt1
Name: xylosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233781
Homologene: 32534
Otog
Name: otogelin
Synonyms: Otgn
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18419
HGNC: HGNC:8516
Homologene: 8421
Cdsn
Name: corneodesmosin
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 386463
HGNC: HGNC:1802
Chtf18
Name: CTF18, chromosome transmission fidelity factor 18
Synonyms: 6030457M03Rik, CTF18
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 214901
Homologene: 32532
Cdkl4
Name: cyclin dependent kinase like 4
Synonyms: LOC381113
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381113
VEGA: 17
Homologene: 28725
Ror2
Name: receptor tyrosine kinase-like orphan receptor 2
Synonyms: Ntrkr2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26564
Homologene: 55831
Aadacl2fm1
Name: AADACL2 family member 1
Synonyms: C130079G13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229333
Cyld
Name: CYLD lysine 63 deubiquitinase
Synonyms: CYLD1, 2010013M14Rik, C130039D01Rik, 2900009M21Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74256
HGNC: HGNC:2584
Homologene: 9069
Ergic3
Name: ERGIC and golgi 3
Synonyms: CGI-54, NY-BR-84, 2310015B14Rik, D2Ucla1, Sdbcag84
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66366
Homologene: 5289
Med16
Name: mediator complex subunit 16
Synonyms: 95kDa, Trap95, Thrap5
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216154
Homologene: 64602
Disp3
Name: dispatched RND transporter family member 3
Synonyms: G630052C06Rik, Ptchd2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242748
Homologene: 25712
Cttnbp2
Name: cortactin binding protein 2
Synonyms: ORF4, Cortbp2, 9130022E09Rik, 4732477G22Rik, 3010022N24Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 30785
Homologene: 14125
Cfap20dc
Name: CFAP20 domain containing
Synonyms: 4930452B06Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 74430
Homologene: 18873
Rgs22
Name: regulator of G-protein signalling 22
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 626596
Homologene: 75047
Tmc1
Name: transmembrane channel-like gene family 1
Synonyms: Bth, Beethoven, 4933416G09Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13409
VEGA: 19
Homologene: 23670
Bmp2k
Name: BMP2 inducible kinase
Synonyms: BIKE, 4933417M22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 140780
Homologene: 34134
Aldh1a3
Name: aldehyde dehydrogenase family 1, subfamily A3
Synonyms: ALDH6, V1, retinaldehyde dehydrogenase 3, RALDH3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56847
HGNC: HGNC:409
Homologene: 68080
Itih4
Name: inter alpha-trypsin inhibitor, heavy chain 4
Synonyms: Itih-4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16427
HGNC: HGNC:6169
Homologene: 1670
Or4k41
Name: olfactory receptor family 4 subfamily K member 41
Synonyms: GA_x6K02T2Q125-72500603-72501520, MOR248-15, Olfr1287
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 257935
Gpbar1
Name: G protein-coupled bile acid receptor 1
Synonyms: GPR131, M-BAR, BG37, TGR5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227289
Homologene: 18125
Rps6ka4
Name: ribosomal protein S6 kinase, polypeptide 4
Synonyms: MSK2, 1110069D02Rik, 90kDa
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56613
VEGA: 19
Homologene: 69288
Cyp3a41a
Name: cytochrome P450, family 3, subfamily a, polypeptide 41A
Synonyms: steroid inducible, Cyp3a41
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53973
HGNC: HGNC:2638
Homologene: 133568
Or6c205
Name: olfactory receptor family 6 subfamily C member 205
Synonyms: GA_x6K02T2PULF-10936819-10937757, MOR111-7, MOR111-6, MOR111-7, Olfr1518, Olfr775
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258538
Homologene: 115559
Zscan4d
Name: zinc finger and SCAN domain containing 4D
Synonyms: EG545913
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 545913
Homologene: 85986
Pus7l
Name: pseudouridylate synthase 7-like
Synonyms: 3000003F02Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78895
Homologene: 12842
Krt27
Name: keratin 27
Synonyms: Krt1-c29, mIRSa3.1, stpm
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16675
Homologene: 7805
Nhlrc1
Name: NHL repeat containing 1
Synonyms: B230309E09Rik, EPM2B, Malin
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105193
VEGA: 13
Homologene: 18439
Ptprv
Name: protein tyrosine phosphatase receptor type V
Synonyms: mOST-PTP, Esp, OST-PTP, OST
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13924
Homologene: 7306
Runx3
Name: runt related transcription factor 3
Synonyms: AML2, Cbfa3, Rx3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12399
Homologene: 37914
Sun3
Name: Sad1 and UNC84 domain containing 3
Synonyms: D630047F21Rik, Sunc1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 194974
Homologene: 51273
Atp6v1g2
Name: ATPase, H+ transporting, lysosomal V1 subunit G2
Synonyms: 1500002D01Rik, NG38, VAG2, Atp6g2, lysosomal 13kDa
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66237
HGNC: HGNC:862
Homologene: 41518
Gm867
Name: predicted gene 867
Synonyms: LOC327769, LOC333670
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 333670
Homologene: 122921
Fkbp14
Name: FK506 binding protein 14
Synonyms: FKBP22
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 231997
Homologene: 23059
Or4c117
Name: olfactory receptor family 4 subfamily C member 117
Synonyms: GA_x6K02T2Q125-50604368-50603433, MOR233-14, Olfr1222
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258177
Homologene: 73988
Gm7135
Name: predicted gene 7135
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 634346
Homologene: 86643
Gm4845
Name: predicted gene 4845
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226472
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • TACCAC to TAC, chromosome 1 at 74,279,545 bp
  • A to T, chromosome 1 at 97,354,076 bp
  • A to G, chromosome 1 at 135,118,848 bp
  • T to A, chromosome 1 at 141,256,885 bp
  • T to C, chromosome 1 at 183,331,115 bp
  • A to G, chromosome 2 at 29,164,550 bp
  • C to T, chromosome 2 at 29,733,655 bp
  • A to G, chromosome 2 at 31,393,025 bp
  • A to T, chromosome 2 at 68,220,992 bp
  • T to C, chromosome 2 at 88,034,661 bp
  • T to C, chromosome 2 at 89,125,338 bp
  • T to A, chromosome 2 at 103,976,143 bp
  • T to C, chromosome 2 at 111,449,683 bp
  • T to C, chromosome 2 at 156,017,718 bp
  • A to G, chromosome 2 at 158,435,508 bp
  • C to A, chromosome 3 at 37,232,504 bp
  • A to C, chromosome 3 at 59,932,740 bp
  • A to G, chromosome 3 at 133,486,759 bp
  • C to T, chromosome 4 at 41,120,167 bp
  • T to C, chromosome 4 at 117,681,036 bp
  • A to G, chromosome 4 at 135,171,135 bp
  • A to G, chromosome 4 at 135,346,720 bp
  • C to T, chromosome 4 at 148,244,216 bp
  • A to G, chromosome 5 at 21,960,426 bp
  • A to T, chromosome 5 at 93,187,694 bp
  • A to G, chromosome 5 at 97,087,085 bp
  • G to T, chromosome 5 at 112,335,747 bp
  • T to A, chromosome 5 at 134,395,627 bp
  • A to G, chromosome 5 at 145,720,048 bp
  • C to A, chromosome 6 at 18,406,526 bp
  • A to G, chromosome 6 at 54,592,958 bp
  • T to C, chromosome 6 at 141,980,873 bp
  • A to T, chromosome 7 at 11,165,347 bp
  • T to G, chromosome 7 at 39,537,080 bp
  • C to T, chromosome 7 at 43,658,972 bp
  • T to C, chromosome 7 at 46,150,867 bp
  • T to C, chromosome 7 at 46,287,991 bp
  • C to T, chromosome 7 at 66,419,179 bp
  • A to T, chromosome 7 at 104,179,529 bp
  • A to T, chromosome 7 at 117,667,342 bp
  • T to G, chromosome 8 at 88,707,232 bp
  • A to T, chromosome 9 at 66,104,905 bp
  • G to T, chromosome 9 at 106,212,340 bp
  • T to A, chromosome 9 at 113,903,916 bp
  • A to G, chromosome 10 at 75,939,802 bp
  • A to G, chromosome 10 at 79,903,005 bp
  • T to C, chromosome 10 at 129,251,272 bp
  • G to A, chromosome 11 at 9,038,311 bp
  • A to G, chromosome 11 at 79,619,671 bp
  • T to C, chromosome 11 at 88,394,655 bp
  • G to A, chromosome 11 at 99,346,896 bp
  • A to T, chromosome 11 at 104,335,564 bp
  • A to G, chromosome 12 at 51,762,497 bp
  • A to G, chromosome 12 at 101,983,493 bp
  • A to G, chromosome 13 at 47,013,740 bp
  • C to T, chromosome 13 at 53,131,918 bp
  • A to G, chromosome 14 at 8,518,736 bp
  • T to A, chromosome 14 at 30,892,287 bp
  • T to C, chromosome 14 at 54,971,671 bp
  • A to C, chromosome 15 at 36,054,876 bp
  • T to A, chromosome 15 at 44,677,667 bp
  • A to G, chromosome 15 at 94,529,488 bp
  • T to A, chromosome 16 at 21,466,469 bp
  • T to C, chromosome 17 at 25,724,566 bp
  • T to C, chromosome 17 at 30,656,985 bp
  • A to G, chromosome 17 at 35,237,172 bp
  • T to C, chromosome 17 at 35,555,429 bp
  • C to A, chromosome 17 at 67,738,834 bp
  • C to A, chromosome 17 at 80,525,335 bp
  • A to G, chromosome 18 at 5,054,025 bp
  • T to G, chromosome 18 at 54,889,890 bp
  • T to A, chromosome 18 at 89,064,085 bp
  • A to T, chromosome 19 at 6,840,310 bp
  • A to C, chromosome 19 at 20,906,955 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4975 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042570-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.