Strain Name:
C57BL/6J-MtgxR5229Btlr/Mmmh
Stock Number:
042802-MU
Citation ID:
RRID:MMRRC_042802-MU
Other Names:
R5229 (G1), C57BL/6J-MtgxR5229Btlr
Major Collection:

Strain Information

Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 6720481N21Rik, C230047C07Rik, 8030492O04Rik, AIP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16396
Homologene: 88442
Gpr26
Name: G protein-coupled receptor 26
Synonyms: 9630036A11Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233919
HGNC: HGNC:4481
Homologene: 17764
Rgs3
Name: regulator of G-protein signaling 3
Synonyms: 4930506N09Rik, C2pa, PDZ-RGS3, RGS3S, C2PA-RGS3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 50780
HGNC: HGNC:9999
Homologene: 32440
Mapkapk5
Name: MAP kinase-activated protein kinase 5
Synonyms: PRAK, MK5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17165
HGNC: HGNC:6889
Homologene: 69077
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: eIF4GII, 1500002J22Rik, 4930523M17Rik, G1-419-52, repro8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Rcc2
Name: regulator of chromosome condensation 2
Synonyms: 2610529N02Rik, 2610510H01Rik, Td60
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 108911
Homologene: 10282
Epb41
Name: erythrocyte membrane protein band 4.1
Synonyms: 4.1R, Elp-1, Elp1, D4Ertd442e, Epb4.1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269587
HGNC: HGNC:3377
Homologene: 44324
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: c-Cbl-associated protein, CAP, Sh3d5, 2310065E01Rik, 9530001P15Rik, Ponsin
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Brwd1
Name: bromodomain and WD repeat domain containing 1
Synonyms: 5330419I02Rik, D530019K20Rik, G1-403-16, Wdr9, repro5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 93871
Homologene: 23130
Tdp1
Name: tyrosyl-DNA phosphodiesterase 1
Synonyms: 4921509N21Rik, SCAN1, 2810481F14Rik, E430034L06Rik, Gm40556
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104884
Homologene: 5424
Nudcd3
Name: NudC domain containing 3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 209586
Homologene: 9097
Heatr5a
Name: HEAT repeat containing 5A
Synonyms: D930036F22Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320487
VEGA: 12
Homologene: 19635
Ube4b
Name: ubiquitination factor E4B
Synonyms: UFD2, 4933406G05Rik, 4930551I19Rik, UFD2a, D4Bwg0973e, Ufd2p
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 63958
Homologene: 107623
Pon1
Name: paraoxonase 1
Synonyms: Pon
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18979
HGNC: HGNC:9204
Homologene: 68058
F2rl2
Name: coagulation factor II thrombin receptor like 2
Synonyms: PAR3, PAR-3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14064
HGNC: HGNC:3539
Homologene: 36151
Pdcd6ip
Name: programmed cell death 6 interacting protein
Synonyms: Alix, AIP1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18571
HGNC: HGNC:8766
Homologene: 22614
Nbn
Name: nibrin
Synonyms: Nbs1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 27354
HGNC: HGNC:7652
Homologene: 1858
Erap1
Name: endoplasmic reticulum aminopeptidase 1
Synonyms: PILSAP, ERAAP, Arts1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 80898
VEGA: 13
Homologene: 56754
Apob
Name: apolipoprotein B
Synonyms: apob-100, apob-48
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Man2a1
Name: mannosidase 2, alpha 1
Synonyms: Mana-2, Mana2, Map-2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17158
HGNC: HGNC:6824
Homologene: 1777
Zfp65
Name: zinc finger protein 65
Synonyms: KRAB5, Zfp71-rs1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 235907
Homologene: 87875
Mcm6
Name: minichromosome maintenance complex component 6
Synonyms: Mcmd6, D1Wsu22e
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17219
HGNC: HGNC:6949
Homologene: 4322
Bub1b
Name: BUB1B, mitotic checkpoint serine/threonine kinase
Synonyms: BUBR1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12236
HGNC: HGNC:1149
Homologene: 933
Scpep1
Name: serine carboxypeptidase 1
Synonyms: 2410018F01Rik, Risc, 4833411K15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74617
Homologene: 41443
Nrbp1
Name: nuclear receptor binding protein 1
Synonyms: B230344L17Rik, Nrbp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 192292
HGNC: HGNC:7993
Homologene: 8373
Kdm4a
Name: lysine (K)-specific demethylase 4A
Synonyms: Jmjd2, D4Ertd222e, JHDM3A, Jmjd2a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230674
Homologene: 27780
Ppp4r2
Name: protein phosphatase 4, regulatory subunit 2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232314
Homologene: 17953
F2
Name: coagulation factor II
Synonyms: prothrombin, Cf-2, Cf2, FII, thrombin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14061
HGNC: HGNC:3535
Homologene: 426
Rnasek
Name: ribonuclease, RNase K
Synonyms: 2310033H11Rik, D11Bwg0434e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 52898
Homologene: 45475
Dpysl3
Name: dihydropyrimidinase-like 3
Synonyms: Ulip1, Ulip, CRMP-4, TUC4, 9430041P20Rik, CRMP4
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 22240
HGNC: HGNC:3015
Homologene: 20361
Myh8
Name: myosin, heavy polypeptide 8, skeletal muscle, perinatal
Synonyms: MyHC-pn, Myhs-p, Myhsp, 4832426G23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17885
HGNC: HGNC:7578
Homologene: 68256
Scn5a
Name: sodium channel, voltage-gated, type V, alpha
Synonyms: Nav1.5c, Nav1.5, mH1, SkM2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20271
Homologene: 22738
Adcy5
Name: adenylate cyclase 5
Synonyms: AC5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224129
VEGA: 16
HGNC: HGNC:236
Homologene: 11213
4930562C15Rik
Name: RIKEN cDNA 4930562C15 gene
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78809
Homologene: 53527
Fam237b
Name: family with sequence similarity 237, member B
Synonyms: Gm29287, Gm8773
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 667705
Or10al5
Name: olfactory receptor family 10 subfamily AL member 5
Synonyms: MOR263-4, GA_x6K02T2PSCP-2211113-2212078, Olfr121
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258622
Homologene: 17195
Rbak
Name: RB-associated KRAB zinc finger
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 57782
Homologene: 23287
Prl2c5
Name: prolactin family 2, subfamily c, member 5
Synonyms: MRP-4, PLF-4, Mrpplf4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107849
Homologene: 40763
Nrdc
Name: nardilysin convertase
Synonyms: NRD-C, Nrd1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230598
HGNC: HGNC:7995
Homologene: 68260
Cnga1
Name: cyclic nucleotide gated channel alpha 1
Synonyms: Cncg
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12788
HGNC: HGNC:2148
Homologene: 55432
Scgb1b24
Name: secretoglobin, family 1B, member 24
Synonyms: Gm12769, Abpa24
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043860
Homologene: 114479
Gm15446
Name: predicted gene 15446
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100642166
Hdac9
Name: histone deacetylase 9
Synonyms: HDRP, Hdac7b, Mitr, D030072B18Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 79221
Homologene: 128578
Or5p54
Name: olfactory receptor family 5 subfamily P member 54
Synonyms: GA_x6K02T2PBJ9-10283869-10284801, MOR204-20, Olfr474
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258488
Tspan2
Name: tetraspanin 2
Synonyms: B230119D02Rik, 6330415F13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70747
Homologene: 21169
Cyp3a11
Name: cytochrome P450, family 3, subfamily a, polypeptide 11
Synonyms: IIIAm1, Cyp3a, Pcn
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13112
HGNC: HGNC:2638
Homologene: 133568
Vmn1r237
Name: vomeronasal 1 receptor 237
Synonyms: V1rf3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171234
Homologene: 64475
Golim4
Name: golgi integral membrane protein 4
Synonyms: GPP130, P138, 3110027H23Rik, Golph4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 73124
Homologene: 8716
Depp1
Name: DEPP1 autophagy regulator
Synonyms: Depp, 8430408G22Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 213393
Homologene: 48491
Gpat3
Name: glycerol-3-phosphate acyltransferase 3
Synonyms: A230097K15Rik, 4933407I02Rik, Agpat9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231510
Homologene: 13099
Slc35g1
Name: solute carrier family 35, member G1
Synonyms: D330039I19Rik, Tmem20
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240660
VEGA: 19
Homologene: 44720
4930444P10Rik
Name: RIKEN cDNA 4930444P10 gene
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75799
Homologene: 90802
Igkv2-95-2
Name: immunoglobulin kappa variable 2-95-2
Synonyms: Gm17533
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 692164
Vmn2r125
Name: vomeronasal 2, receptor 125
Synonyms: Gm20782
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 670059
VEGA: 4
Igkv10-96
Name: immunoglobulin kappa variable 10-96
Synonyms: Gm16637
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 692165
Trav6-5
Name: T cell receptor alpha variable 6-5
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100126464
Lrg1
Name: leucine-rich alpha-2-glycoprotein 1
Synonyms: 2310031E04Rik, Lrhg, 1300008B03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76905
VEGA: 17
Homologene: 36468
Gm9104
Name: predicted gene 9104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 668319
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 16,080,959 bp
  • T to C, chromosome 1 at 128,333,584 bp
  • A to T, chromosome 2 at 91,630,241 bp
  • C to A, chromosome 2 at 118,629,989 bp
  • T to C, chromosome 2 at 155,168,790 bp
  • G to A, chromosome 3 at 75,956,239 bp
  • T to C, chromosome 3 at 102,768,899 bp
  • C to T, chromosome 4 at 15,963,893 bp
  • T to C, chromosome 4 at 62,702,187 bp
  • T to C, chromosome 4 at 88,630,135 bp
  • T to C, chromosome 4 at 109,049,108 bp
  • G to A, chromosome 4 at 118,146,605 bp
  • C to A, chromosome 4 at 131,978,935 bp
  • C to A, chromosome 4 at 138,096,794 bp
  • G to A, chromosome 4 at 140,717,029 bp
  • A to G, chromosome 4 at 149,387,178 bp
  • C to T, chromosome 4 at 156,351,038 bp
  • A to T, chromosome 5 at 5,575,565 bp
  • T to C, chromosome 5 at 31,251,093 bp
  • A to G, chromosome 5 at 72,609,500 bp
  • G to A, chromosome 5 at 100,883,424 bp
  • T to G, chromosome 5 at 109,943,170 bp
  • T to C, chromosome 5 at 121,533,391 bp
  • A to G, chromosome 5 at 143,174,162 bp
  • G to T, chromosome 5 at 145,855,135 bp
  • C to T, chromosome 6 at 5,177,295 bp
  • A to G, chromosome 6 at 68,632,239 bp
  • A to G, chromosome 6 at 68,648,111 bp
  • C to A, chromosome 6 at 100,865,215 bp
  • T to C, chromosome 6 at 116,652,031 bp
  • A to T, chromosome 7 at 33,744,095 bp
  • A to G, chromosome 7 at 107,955,169 bp
  • A to T, chromosome 7 at 131,984,247 bp
  • A to T, chromosome 9 at 113,678,333 bp
  • A to G, chromosome 9 at 119,535,976 bp
  • A to G, chromosome 11 at 6,193,238 bp
  • A to T, chromosome 11 at 67,284,484 bp
  • A to T, chromosome 11 at 70,239,660 bp
  • A to T, chromosome 11 at 88,937,045 bp
  • A to G, chromosome 12 at 7,977,806 bp
  • A to C, chromosome 12 at 34,437,164 bp
  • A to T, chromosome 12 at 51,947,978 bp
  • A to G, chromosome 12 at 99,893,660 bp
  • G to A, chromosome 13 at 13,185,856 bp
  • A to T, chromosome 13 at 67,708,810 bp
  • G to A, chromosome 13 at 74,660,375 bp
  • A to T, chromosome 13 at 95,700,687 bp
  • T to G, chromosome 14 at 53,491,588 bp
  • A to G, chromosome 16 at 4,850,051 bp
  • A to G, chromosome 16 at 35,269,070 bp
  • C to A, chromosome 16 at 96,002,209 bp
  • C to A, chromosome 17 at 21,314,371 bp
  • A to G, chromosome 17 at 37,752,301 bp
  • A to G, chromosome 17 at 45,466,133 bp
  • A to G, chromosome 17 at 56,120,154 bp
  • A to T, chromosome 17 at 64,710,734 bp
  • C to A, chromosome 18 at 43,332,951 bp
  • A to G, chromosome 19 at 38,402,632 bp
  • A to G, chromosome 19 at 40,340,707 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5229 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042802-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.