Strain Name:
C57BL/6J-MtgxR5422Btlr/Mmmh
Stock Number:
042846-MU
Citation ID:
RRID:MMRRC_042846-MU
Other Names:
R5422 (G1), C57BL/6J-MtgxR5422Btlr
Major Collection:

Strain Information

Fkbp11
Name: FK506 binding protein 11
Synonyms: 1110002O23Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66120
VEGA: 15
Homologene: 9581
Kcnj5
Name: potassium inwardly-rectifying channel, subfamily J, member 5
Synonyms: Kir3.4, GIRK4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 16521
VEGA: 9
HGNC: HGNC:6266
Homologene: 20248
Map7
Name: microtubule-associated protein 7
Synonyms: E-MAP-115, ste, mste, mshi, Mtap7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17761
VEGA: 10
HGNC: HGNC:6869
Homologene: 20851
Ewsr1
Name: Ewing sarcoma breakpoint region 1
Synonyms: Ews
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14030
HGNC: HGNC:3508
Homologene: 134632
Gch1
Name: GTP cyclohydrolase 1
Synonyms: GTPCH, GTP-CH
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14528
VEGA: 14
HGNC: HGNC:4193
Homologene: 132
Mapkapk5
Name: MAP kinase-activated protein kinase 5
Synonyms: PRAK, MK5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17165
HGNC: HGNC:6889
Homologene: 69077
Micu1
Name: mitochondrial calcium uptake 1
Synonyms: C730016L05Rik, Cbara1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216001
HGNC: HGNC:1530
Homologene: 4431
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107182
Homologene: 31978
Tcf12
Name: transcription factor 12
Synonyms: ME1, ALF1, HEB, HTF4, HTF-4, REB, bHLHb20, HEBAlt
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21406
Homologene: 40774
Ranbp9
Name: RAN binding protein 9
Synonyms: IBAP-1, RanBPM
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 56705
VEGA: 13
Homologene: 38057
Kif15
Name: kinesin family member 15
Synonyms: N-10 kinesin, HKLP2, 3930402I10Rik, 3110023M17Rik, Knsl7
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 209737
VEGA: 9
Homologene: 23210
Utp18
Name: UTP18 small subunit processome component
Synonyms: 6230425C22Rik, Wdr50
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217109
Homologene: 41087
Arhgap32
Name: Rho GTPase activating protein 32
Synonyms: p200RhoGAP, GC-GAP, PX-RICS, 3426406O18Rik, Grit
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330914
Homologene: 8812
Ereg
Name: epiregulin
Synonyms: EPR
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13874
HGNC: HGNC:3443
Homologene: 1097
Ambn
Name: ameloblastin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11698
HGNC: HGNC:452
Homologene: 7625
Tjp1
Name: tight junction protein 1
Synonyms: ZO-1, ZO1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21872
Homologene: 2445
Zfp335
Name: zinc finger protein 335
Synonyms: 1810045J01Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329559
Homologene: 11129
Tph2
Name: tryptophan hydroxylase 2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216343
Homologene: 27831
Adgrg5
Name: adhesion G protein-coupled receptor G5
Synonyms: LOC382045, PGR27, Gpr114
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382045
Homologene: 17828
Zfp54
Name: zinc finger protein 54
Synonyms: clone 18, KRAB10, Zfp-54, Zfp76
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22712
Homologene: 129791
Hnrnpa2b1
Name: heterogeneous nuclear ribonucleoprotein A2/B1
Synonyms: Hnrpa2, 9130414A06Rik, Hnrpa2b1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 53379
HGNC: HGNC:5033
Homologene: 22992
Vps51
Name: VPS51 GARP complex subunit
Synonyms: 3110057M17Rik, 1110014N23Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 68505
HGNC: HGNC:1172
Homologene: 34675
Magi3
Name: membrane associated guanylate kinase, WW and PDZ domain containing 3
Synonyms: 4732496O19Rik, 6530407C02Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99470
Homologene: 26431
Thoc6
Name: THO complex 6
Synonyms: F830014G06Rik, Wdr58
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 386612
Homologene: 11533
Pcdhb16
Name: protocadherin beta 16
Synonyms: PcdhbP, Pcdhb8
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93887
Homologene: 99843
Clu
Name: clusterin
Synonyms: testosterone repressed prostate message-2, SP-40, complement lysis inhibitor, Sgp-2, ApoJ, Apolipoprotein J, Cli, Sugp-2, Sgp2, D14Ucla3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12759
HGNC: HGNC:2095
Homologene: 1382
Mapk11
Name: mitogen-activated protein kinase 11
Synonyms: Prkm11, p38beta, Sapk2, P38b
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19094
VEGA: 15
HGNC: HGNC:6873
Homologene: 55684
Epha5
Name: Eph receptor A5
Synonyms: Cek7, bsk, Els1, Rek7, Hek7, Ehk1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13839
HGNC: HGNC:3389
Homologene: 55824
Slc45a2
Name: solute carrier family 45, member 2
Synonyms: Dbr, bls, Aim1, Aim-1, dominant brown, blanc-sale, Matp, Oca4
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 22293
Homologene: 9412
Elf3
Name: E74-like factor 3
Synonyms: jen, ESX, ESE-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13710
HGNC: HGNC:3318
Homologene: 3265
Btnl6
Name: butyrophilin-like 6
Synonyms: NG13, Gm6519
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 624681
Homologene: 106849
Myh7b
Name: myosin, heavy chain 7B, cardiac muscle, beta
Synonyms: Myh14
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 668940
Homologene: 66117
Fat4
Name: FAT atypical cadherin 4
Synonyms: 6030410K14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329628
Homologene: 14377
Nprl2
Name: NPR2 like, GATOR1 complex subunit
Synonyms: G21, NPR2L, NPRL2, 2810446G01Rik, Tusc4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56032
Homologene: 4771
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
Nalcn
Name: sodium leak channel, non-selective
Synonyms: A530023G15Rik, Vgcnl1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 338370
VEGA: 14
Homologene: 21832
Tbc1d17
Name: TBC1 domain family, member 17
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233204
Homologene: 11656
Map6
Name: microtubule-associated protein 6
Synonyms: F-STOP, STOP, 2810411E12Rik, Mtap6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17760
HGNC: HGNC:6868
Homologene: 7850
Fgl2
Name: fibrinogen-like protein 2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14190
HGNC: HGNC:3696
Homologene: 4864
Clk3
Name: CDC-like kinase 3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102414
VEGA: 9
HGNC: HGNC:2071
Homologene: 101534
Plcl1
Name: phospholipase C-like 1
Synonyms: PLC-L, C230017K02Rik, PRIP-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227120
HGNC: HGNC:9063
Homologene: 38155
Ghrhr
Name: growth hormone releasing hormone receptor
Synonyms: Ghrfr
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14602
HGNC: HGNC:4266
Homologene: 640
Ogfr
Name: opioid growth factor receptor
Synonyms: 2010013E17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72075
Homologene: 7199
Adamts18
Name: ADAM metallopeptidase with thrombospondin type 1 motif 18
Synonyms: E130314N14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 208936
Homologene: 65241
Atp8b5
Name: ATPase, class I, type 8B, member 5
Synonyms: FetA, 4930417M19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320571
Homologene: 99882
Thsd7b
Name: thrombospondin, type I, domain containing 7B
Synonyms: 1700074E13Rik, D130067I03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210417
Homologene: 18180
Sec16b
Name: SEC16 homolog B, endoplasmic reticulum export factor
Synonyms: Rgpr-p117, Rgpr, Lztr2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 89867
Homologene: 13227
Pdzrn4
Name: PDZ domain containing RING finger 4
Synonyms: SAMCAP3L, LNX4, 1110017D07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239618
VEGA: 15
Homologene: 85433
Usp9y
Name: ubiquitin specific peptidase 9, Y chromosome
Synonyms: Dffry, Fafl2
Type: Gene
Species: Mouse
Chromosome: Y
NCBI: 107868
Homologene: 68408
Parp14
Name: poly (ADP-ribose) polymerase family, member 14
Synonyms: 1600029O10Rik, collaborator of Stat6, CoaSt6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 547253
Homologene: 19697
Or8c10
Name: olfactory receptor family 8 subfamily C member 10
Synonyms: GA_x6K02T2MYUG-19447-18473, MOR170-14, Olfr250, MOR170-8, GA_x6K02T2PVTD-32060891-32061865, Olfr899
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 404312
VEGA: 9
Homologene: 74151
Thoc2l
Name: THO complex subunit 2-like
Synonyms: Gm3179, BC005561
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100042165
Scart1
Name: scavenger receptor family member expressed on T cells 1
Synonyms: E430002D04Rik, Cd163l1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244233
Homologene: 129778
Serpine2
Name: serine (or cysteine) peptidase inhibitor, clade E, member 2
Synonyms: nexin, PN-1, PI7, protease nexin 1, Spi4, B230326M24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20720
HGNC: HGNC:8951
Homologene: 21247
Or6b13
Name: olfactory receptor family 6 subfamily B member 13
Synonyms: GA_x6K02T2PBJ9-42354580-42353624, MOR103-14P, Olfr524
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258055
Homologene: 79347
Rassf5
Name: Ras association (RalGDS/AF-6) domain family member 5
Synonyms: 1300019G20Rik, Nore1B, Nore1A, Rapl
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54354
Homologene: 10296
Ptpn9
Name: protein tyrosine phosphatase, non-receptor type 9
Synonyms: Meg2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56294
VEGA: 9
HGNC: HGNC:9661
Homologene: 2121
Gje1
Name: gap junction protein, epsilon 1
Synonyms: AEY12, connexin 23, Cx23, D230044M03Rik, Gsfaey12, Gjf1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76743
Homologene: 87405
Cyp2w1
Name: cytochrome P450, family 2, subfamily w, polypeptide 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 545817
Homologene: 69239
Zfp607b
Name: zinc finger protein 607B
Synonyms: C030039L03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 112415
Homologene: 134321
Sgms1
Name: sphingomyelin synthase 1
Synonyms: SMS1, 9530058O11Rik, SMS1gamma, SMS1beta, SMS1alpha2, SMS1alpha1, Tmem23
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 208449
Homologene: 27040
1700120B22Rik
Name: RIKEN cDNA 1700120B22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 641387
Ghdc
Name: GH3 domain containing
Synonyms: D11Lgp1e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 80860
Homologene: 32760
Sh3bp5
Name: SH3-domain binding protein 5 (BTK-associated)
Synonyms: Sab
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 24056
Homologene: 23450
Rasgef1a
Name: RasGEF domain family, member 1A
Synonyms: 6330404M18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70727
Homologene: 17067
Agmat
Name: agmatinase
Synonyms: 5033405N08Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 75986
Homologene: 99855
Gbx1
Name: gastrulation brain homeobox 1
Synonyms: Gbx-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231044
HGNC: HGNC:4185
Homologene: 41063
Fn3k
Name: fructosamine 3 kinase
Synonyms: 2310074G21Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 63828
Homologene: 23336
Gm26899
Name: predicted gene, 26899
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Catspere1
Name: cation channel sperm associated auxiliary subunit epsilon 1
Synonyms: Catspere, Gm7068
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 631584
Homologene: 52161
Gm8032
Name: predicted gene 8032
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 101055806
Homologene: 115686
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 55,697,384 bp
  • C to A, chromosome 1 at 79,816,875 bp
  • T to C, chromosome 1 at 79,821,489 bp
  • T to C, chromosome 1 at 129,921,334 bp
  • C to A, chromosome 1 at 131,181,174 bp
  • C to T, chromosome 1 at 135,255,040 bp
  • T to A, chromosome 1 at 157,538,262 bp
  • A to T, chromosome 1 at 177,907,760 bp
  • A to G, chromosome 2 at 82,982,228 bp
  • T to C, chromosome 2 at 120,535,484 bp
  • A to C, chromosome 2 at 155,631,034 bp
  • T to C, chromosome 2 at 164,907,730 bp
  • G to T, chromosome 2 at 180,595,274 bp
  • A to T, chromosome 2 at 180,595,275 bp
  • A to G, chromosome 3 at 38,887,245 bp
  • C to T, chromosome 3 at 104,051,368 bp
  • T to A, chromosome 4 at 43,366,644 bp
  • C to A, chromosome 4 at 141,755,833 bp
  • T to G, chromosome 5 at 21,375,810 bp
  • C to T, chromosome 5 at 24,504,669 bp
  • A to T, chromosome 5 at 84,331,490 bp
  • T to C, chromosome 5 at 88,464,511 bp
  • T to C, chromosome 5 at 91,074,807 bp
  • T to C, chromosome 5 at 104,519,646 bp
  • T to A, chromosome 5 at 121,531,722 bp
  • T to C, chromosome 5 at 139,352,773 bp
  • A to T, chromosome 6 at 51,465,228 bp
  • A to G, chromosome 6 at 55,388,203 bp
  • T to A, chromosome 6 at 118,088,134 bp
  • A to G, chromosome 7 at 27,702,388 bp
  • A to T, chromosome 7 at 44,848,868 bp
  • A to T, chromosome 7 at 65,302,967 bp
  • A to G, chromosome 7 at 99,269,002 bp
  • A to T, chromosome 7 at 140,202,392 bp
  • A to T, chromosome 7 at 140,224,155 bp
  • T to A, chromosome 8 at 94,933,952 bp
  • T to C, chromosome 8 at 113,698,974 bp
  • A to G, chromosome 9 at 32,247,230 bp
  • A to G, chromosome 9 at 32,317,705 bp
  • A to T, chromosome 9 at 38,367,974 bp
  • T to A, chromosome 9 at 57,033,157 bp
  • A to G, chromosome 9 at 57,765,438 bp
  • A to T, chromosome 9 at 71,869,038 bp
  • C to A, chromosome 9 at 107,543,597 bp
  • T to A, chromosome 9 at 122,984,889 bp
  • G to A, chromosome 10 at 14,716,684 bp
  • G to T, chromosome 10 at 20,266,766 bp
  • C to A, chromosome 10 at 59,740,794 bp
  • T to A, chromosome 10 at 63,243,101 bp
  • T to A, chromosome 10 at 115,079,764 bp
  • A to G, chromosome 11 at 5,080,668 bp
  • T to C, chromosome 11 at 93,885,660 bp
  • C to A, chromosome 11 at 100,769,194 bp
  • C to A, chromosome 11 at 121,450,122 bp
  • A to T, chromosome 13 at 43,419,626 bp
  • C to A, chromosome 14 at 31,377,495 bp
  • T to C, chromosome 14 at 42,559,100 bp
  • C to T, chromosome 14 at 47,157,449 bp
  • A to G, chromosome 14 at 65,975,602 bp
  • A to T, chromosome 14 at 123,515,365 bp
  • C to T, chromosome 15 at 11,027,785 bp
  • A to G, chromosome 15 at 89,146,285 bp
  • G to A, chromosome 15 at 92,677,621 bp
  • A to T, chromosome 15 at 98,728,108 bp
  • T to C, chromosome 16 at 35,866,175 bp
  • C to A, chromosome 17 at 21,434,526 bp
  • A to T, chromosome 17 at 23,669,480 bp
  • C to T, chromosome 17 at 34,514,107 bp
  • A to T, chromosome 18 at 37,479,867 bp
  • T to G, chromosome 19 at 6,071,033 bp
  • G to T, chromosome 19 at 32,159,832 bp
  • A to T, chromosome 19 at 36,951,107 bp
  • T to C, chromosome Y at 1,314,676 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5422 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042846-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.