Strain Name:
C57BL/6J-MtgxR5308Btlr/Mmmh
Stock Number:
042891-MU
Citation ID:
RRID:MMRRC_042891-MU
Other Names:
R5308 (G1), C57BL/6J-MtgxR5308Btlr
Major Collection:

Strain Information

Eno2
Name: enolase 2, gamma neuronal
Synonyms: Eno-2, D6Ertd375e, NSE
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13807
HGNC: HGNC:3353
Homologene: 74414
Paics
Name: phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoribosylaminoimidazole, succinocarboxamide synthetase
Synonyms: ADE2H1, PAIS, AIRC, 2610511I09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67054
HGNC: HGNC:8587
Homologene: 4710
Usp28
Name: ubiquitin specific peptidase 28
Synonyms: 9830148O20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235323
VEGA: 9
Homologene: 10840
Cdk13
Name: cyclin dependent kinase 13
Synonyms: 2310015O17Rik, Cdc2l5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69562
VEGA: 13
HGNC: HGNC:1733
Homologene: 135707
Xpa
Name: xeroderma pigmentosum, complementation group A
Synonyms: Xpac
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22590
Homologene: 37298
Usp32
Name: ubiquitin specific peptidase 32
Synonyms: 6430526O11Rik, 2900074J03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237898
Homologene: 13066
Rfc1
Name: replication factor C (activator 1) 1
Synonyms: RFC140, Alp145, 140kDa, Recc1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19687
HGNC: HGNC:9969
Homologene: 2187
Pcnt
Name: pericentrin (kendrin)
Synonyms: Pcnt2, m275Asp, m239Asp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18541
VEGA: 10
Rbm27
Name: RNA binding motif protein 27
Synonyms: Psc1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225432
VEGA: 18
Homologene: 35410
Tbc1d8
Name: TBC1 domain family, member 8
Synonyms: BUB2-like protein 1, HBLP1, AD3, GRAM domain
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54610
Homologene: 31421
Prrc2a
Name: proline-rich coiled-coil 2A
Synonyms: G2, Bat-2, D17H6S51E, Wbp12, 3110039B05Rik, Bat2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 53761
Homologene: 10567
Itpr1
Name: inositol 1,4,5-trisphosphate receptor 1
Synonyms: P400, IP3R1, Itpr-1, Ip3r, Pcp-1, opt, InsP3R type I, Pcp1, wblo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16438
HGNC: HGNC:6180
Homologene: 1673
Ric8b
Name: RIC8 guanine nucleotide exchange factor B
Synonyms: Ric-8, Ric-8b
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237422
VEGA: 10
Homologene: 23080
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, Ankyrin-3, AnkG, 2900054D09Rik, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Strn3
Name: striatin, calmodulin binding protein 3
Synonyms: SG2NA
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 94186
VEGA: 12
Homologene: 82078
Gcfc2
Name: GC-rich sequence DNA binding factor 2
Synonyms: AW146020
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330361
HGNC: HGNC:1317
Homologene: 2411
Myo9a
Name: myosin IXa
Synonyms: 4732465J09Rik, C130068I12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270163
HGNC: HGNC:7608
Homologene: 21371
Dmbt1
Name: deleted in malignant brain tumors 1
Synonyms: CRP-[b], CRP-[a], ebnerin, hensin, vomeroglandin, Crpd, MUCLIN, gp300
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12945
HGNC: HGNC:2926
Homologene: 68990
Scyl2
Name: SCY1-like 2 (S. cerevisiae)
Synonyms: D10Ertd802e, CVAK104
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 213326
Homologene: 13349
Angptl3
Name: angiopoietin-like 3
Synonyms: hypl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 30924
HGNC: HGNC:491
Homologene: 8499
Glb1l2
Name: galactosidase, beta 1-like 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244757
Homologene: 71790
Cdc37
Name: cell division cycle 37
Synonyms: p50Cdc37
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12539
VEGA: 9
HGNC: HGNC:1735
Homologene: 38268
Snx11
Name: sorting nexin 11
Synonyms: 4933439F10Rik, A930041K09Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74479
Homologene: 12568
Plekho2
Name: pleckstrin homology domain containing, family O member 2
Synonyms: Plekhq1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102595
VEGA: 9
Homologene: 11870
Grn
Name: granulin
Synonyms: acrogranulin, epithelin, PC cell-derived growth factor, progranulin, Pgrn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14824
HGNC: HGNC:4601
Homologene: 1577
Ckap4
Name: cytoskeleton-associated protein 4
Synonyms: CLIMP-63, P63, 5630400A09Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216197
VEGA: 10
Homologene: 4970
Itga11
Name: integrin alpha 11
Synonyms: 4732459H24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319480
HGNC: HGNC:6136
Homologene: 8151
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: Mdnah5, b2b1154Clo, b2b1134Clo, b2b1537Clo, b2b1565Clo, Dnahc5, b2b3491Clo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: lrd, b2b598Clo, b2b1203Clo, b2b1279Clo, b2b1289Clo, b2b1727Clo, Dnahc11, avc4
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Klra3
Name: killer cell lectin-like receptor, subfamily A, member 3
Synonyms: 5E6, Ly49C, Nk-2, Ly49c, NK-2.1, Nk2.1, Nk2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16634
Homologene: 110821
Garre1
Name: granule associated Rac and RHOG effector 1
Synonyms: 4931406P16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233103
Homologene: 8795
Slc4a1
Name: solute carrier family 4 (anion exchanger), member 1
Synonyms: erythrocyte membrane protein band 3, band 3, Empb3, Ae1, CD233, l11Jus51
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20533
Homologene: 133556
Cplane1
Name: ciliogenesis and planar polarity effector 1
Synonyms: b2b012Clo, Jbts17, Hug, 2410089E03Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 73692
Homologene: 11315
Col6a3
Name: collagen, type VI, alpha 3
Synonyms: Col6a-3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12835
HGNC: HGNC:2213
Homologene: 37917
Csf3r
Name: colony stimulating factor 3 receptor
Synonyms: G-CSFR, Csfgr, Cd114
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12986
HGNC: HGNC:2439
Homologene: 601
Slc20a1
Name: solute carrier family 20, member 1
Synonyms: Glvr-1, Glvr1, PiT-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20515
Homologene: 38049
Sacs
Name: sacsin
Synonyms: E130115J16Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50720
Matn3
Name: matrilin 3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 17182
HGNC: HGNC:6909
Homologene: 1785
1700006A11Rik
Name: RIKEN cDNA 1700006A11 gene
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71824
Abcd4
Name: ATP-binding cassette, sub-family D member 4
Synonyms: P69r, Pxmp1l
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 19300
VEGA: 12
HGNC: HGNC:68
Homologene: 3703
Nxpe3
Name: neurexophilin and PC-esterase domain family, member 3
Synonyms: LOC208684, LOC385658, Fam55c
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 385658
VEGA: 16
Homologene: 17030
Hexd
Name: hexosaminidase D
Synonyms: Hexdc
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 238023
Homologene: 68120
Cimap3
Name: ciliary microtubule associated protein 3
Synonyms: pitchfork, 1700027A23Rik, Pifo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100503311
Homologene: 18759
Iqsec1
Name: IQ motif and Sec7 domain 1
Synonyms: cI-43, D6Ertd349e, BRAG2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232227
Homologene: 82429
Mad2l1
Name: MAD2 mitotic arrest deficient-like 1
Synonyms: MAD2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56150
HGNC: HGNC:6763
Homologene: 1768
Tbc1d4
Name: TBC1 domain family, member 4
Synonyms: 5930406J04Rik, AS160
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 210789
Homologene: 45451
Zfp85
Name: zinc finger protein 85
Synonyms: Zfp71, KRAB19, Zfp85-rs1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 22746
Homologene: 133709
Ercc4
Name: excision repair cross-complementing rodent repair deficiency, complementation group 4
Synonyms: Xpf
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 50505
HGNC: HGNC:3436
Homologene: 3836
Igfbp1
Name: insulin-like growth factor binding protein 1
Synonyms: IGFBP-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16006
HGNC: HGNC:5469
Homologene: 498
Cyp2d37-ps
Name: cytochrome P450, family 2, subfamily d, polypeptide 37, pseudogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 627860
VEGA: 15
Cfap61
Name: cilia and flagella associated protein 61
Synonyms: 4930529M08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78774
Or4c103
Name: olfactory receptor family 4 subfamily C member 103
Synonyms: GA_x6K02T2Q125-50163514-50162588, MOR230-4, MOR230-12_p, Olfr1195
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258748
Homologene: 103782
Or4k45
Name: olfactory receptor family 4 subfamily K member 45
Synonyms: GA_x6K02T2Q125-72616944-72616006, MOR248-10, Olfr1295
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258398
Homologene: 74058
Stard4
Name: StAR related lipid transfer domain containing 4
Synonyms: 9030213J02Rik, 4632419C16Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 170459
Homologene: 15874
4921504E06Rik
Name: RIKEN cDNA 4921504E06 gene
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70909
Homologene: 87049
Krtap4-22
Name: keratin associated protein 4-22
Synonyms: Gm11595
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100040276
Homologene: 124486
Tmem45b
Name: transmembrane protein 45b
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235135
VEGA: 9
Homologene: 16349
Fcgr2b
Name: Fc receptor, IgG, low affinity IIb
Synonyms: CD32, FcgRII, Fc[g]RII, Fc gamma RIIB, FcgammaRIIB, Fcgr2, Fcr-2, Ly-m20, Ly-17, LyM-1, Fcr-3, Fcgr2a, F630109E10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14130
Homologene: 2974
Tas2r121
Name: taste receptor, type 2, member 121
Synonyms: Tas2r21, mT2r48, mGR21, T2R21
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 387349
Homologene: 41542
Or1j16
Name: olfactory receptor family 1 subfamily J member 16
Synonyms: GA_x6K02T2NLDC-33334179-33335114, MOR136-7, Olfr345
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258947
Homologene: 45069
Alg9
Name: ALG9 alpha-1,2-mannosyltransferase
Synonyms: 8230402H15Rik, B430313H07Rik, Dibd1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102580
Homologene: 6756
Stx17
Name: syntaxin 17
Synonyms: 6330411F21Rik, 4833418L03Rik, 9030425C21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67727
Homologene: 9917
Garin5a
Name: golgi associated RAB2 interactor 5A
Synonyms: 1700021N13Rik, 0610007G24Rik, 1700021P22Rik, Fam71e1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75538
Homologene: 19259
Or3a1c
Name: olfactory receptor family 3 subfamily A member 1C
Synonyms: GA_x6K02T2P1NL-4307199-4308146, MOR255-4, Olfr402
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258703
HGNC: HGNC:8282
Homologene: 1915
Romo1
Name: reactive oxygen species modulator 1
Synonyms: 2010100O12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67067
Homologene: 7096
Sh3bp5
Name: SH3-domain binding protein 5 (BTK-associated)
Synonyms: Sab
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 24056
Homologene: 23450
Rpl26
Name: ribosomal protein L26
Synonyms: SIG-20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 19941
Homologene: 113207
Snx18
Name: sorting nexin 18
Synonyms: Snag1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 170625
VEGA: 13
Homologene: 14164
Plscr5
Name: phospholipid scramblase family, member 5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 100504689
Homologene: 28688
BC048644
Name: cDNA sequence BC048644
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 407789
RP23-357H5.5
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 39,389,409 bp
  • G to C, chromosome 1 at 90,801,531 bp
  • T to G, chromosome 1 at 170,965,710 bp
  • T to C, chromosome 2 at 19,524,081 bp
  • T to A, chromosome 2 at 36,640,694 bp
  • T to C, chromosome 2 at 88,683,405 bp
  • C to T, chromosome 2 at 111,564,554 bp
  • C to T, chromosome 2 at 129,200,311 bp
  • G to A, chromosome 2 at 146,109,988 bp
  • C to A, chromosome 2 at 156,144,553 bp
  • T to C, chromosome 3 at 106,001,103 bp
  • C to G, chromosome 3 at 124,406,350 bp
  • T to A, chromosome 4 at 46,185,659 bp
  • A to T, chromosome 4 at 48,182,851 bp
  • C to A, chromosome 4 at 99,034,486 bp
  • A to G, chromosome 4 at 126,035,344 bp
  • T to A, chromosome 5 at 65,279,461 bp
  • T to C, chromosome 5 at 76,956,632 bp
  • T to C, chromosome 6 at 66,537,691 bp
  • T to C, chromosome 6 at 81,943,543 bp
  • G to A, chromosome 6 at 90,662,878 bp
  • A to T, chromosome 6 at 108,356,511 bp
  • C to A, chromosome 6 at 124,767,093 bp
  • T to C, chromosome 6 at 130,334,307 bp
  • G to A, chromosome 6 at 132,700,517 bp
  • T to A, chromosome 7 at 34,245,755 bp
  • T to C, chromosome 7 at 44,500,182 bp
  • T to A, chromosome 7 at 131,041,021 bp
  • G to T, chromosome 8 at 121,917,686 bp
  • A to T, chromosome 9 at 21,140,764 bp
  • C to T, chromosome 9 at 26,764,759 bp
  • T to C, chromosome 9 at 31,429,084 bp
  • T to C, chromosome 9 at 49,037,201 bp
  • T to C, chromosome 9 at 50,822,711 bp
  • T to A, chromosome 9 at 59,863,961 bp
  • T to A, chromosome 9 at 62,755,769 bp
  • T to A, chromosome 9 at 65,558,675 bp
  • T to C, chromosome 9 at 92,198,512 bp
  • G to A, chromosome 9 at 109,789,449 bp
  • G to A, chromosome 10 at 70,002,565 bp
  • A to C, chromosome 10 at 76,356,325 bp
  • T to C, chromosome 10 at 84,528,374 bp
  • T to A, chromosome 10 at 84,947,747 bp
  • A to T, chromosome 10 at 89,642,007 bp
  • G to A, chromosome 11 at 7,199,919 bp
  • T to A, chromosome 11 at 68,904,458 bp
  • T to A, chromosome 11 at 74,155,571 bp
  • T to A, chromosome 11 at 85,017,718 bp
  • A to G, chromosome 11 at 96,770,709 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • T to A, chromosome 11 at 102,359,077 bp
  • A to G, chromosome 11 at 102,436,192 bp
  • T to A, chromosome 11 at 121,222,269 bp
  • CGGGGCTCGGGGGC to CGGGGC, chromosome 12 at 8,952,308 bp
  • A to G, chromosome 12 at 51,629,385 bp
  • C to T, chromosome 12 at 84,603,293 bp
  • A to G, chromosome 12 at 118,085,680 bp
  • T to C, chromosome 13 at 17,772,313 bp
  • C to T, chromosome 13 at 67,748,855 bp
  • G to A, chromosome 13 at 113,616,847 bp
  • C to A, chromosome 14 at 31,377,495 bp
  • T to C, chromosome 14 at 61,192,400 bp
  • T to A, chromosome 14 at 101,462,833 bp
  • G to A, chromosome 15 at 8,260,690 bp
  • A to T, chromosome 15 at 28,229,651 bp
  • T to C, chromosome 15 at 82,689,811 bp
  • G to T, chromosome 16 at 13,130,164 bp
  • C to T, chromosome 16 at 55,866,471 bp
  • C to T, chromosome 17 at 35,161,047 bp
  • G to T, chromosome 18 at 33,203,625 bp
  • T to A, chromosome 18 at 42,327,210 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5308 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042891-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.