Strain Name:
C57BL/6J-MtgxR5324Btlr/Mmmh
Stock Number:
042907-MU
Citation ID:
RRID:MMRRC_042907-MU
Other Names:
R5324 (G1), C57BL/6J-MtgxR5324Btlr
Major Collection:

Strain Information

Akap10
Name: A kinase anchor protein 10
Synonyms: D-AKAP2, 1500031L16Rik, B130049N18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56697
HGNC: HGNC:368
Homologene: 32452
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: alpha-fodrin, Spna-2, 2610027H02Rik, Spna2, alphaII-spectrin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20740
Homologene: 2353
Zfp384
Name: zinc finger protein 384
Synonyms: C130073D16Rik, Ciz, Nmp4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 269800
Homologene: 15849
Psma2
Name: proteasome subunit alpha 2
Synonyms: Lmpc3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19166
VEGA: 13
HGNC: HGNC:9531
Homologene: 2081
Fmn2
Name: formin 2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54418
Homologene: 137334
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: E130014J05Rik, Ayu17-449
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214133
Homologene: 49498
Cbl
Name: Casitas B-lineage lymphoma
Synonyms: Cbl-2, c-Cbl, 4732447J05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12402
HGNC: HGNC:1541
Homologene: 3802
Serpini1
Name: serine (or cysteine) peptidase inhibitor, clade I, member 1
Synonyms: Neuroserpin, PI12, Spi17, Ns
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20713
HGNC: HGNC:8943
Homologene: 21045
N6amt1
Name: N-6 adenine-specific DNA methyltransferase 1 (putative)
Synonyms: 5830445C04Rik, Pred28, Hemk2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67768
Homologene: 5637
Bmp2
Name: bone morphogenetic protein 2
Synonyms: Bmp2a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12156
HGNC: HGNC:1069
Homologene: 926
Epg5
Name: ectopic P-granules 5 autophagy tethering factor
Synonyms: 5430411K18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 100502841
VEGA: 18
Homologene: 14575
Pabpc1
Name: poly(A) binding protein, cytoplasmic 1
Synonyms: Pabpl1, Pabp1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18458
Homologene: 37638
Cyp1a1
Name: cytochrome P450, family 1, subfamily a, polypeptide 1
Synonyms: cytochrome P450 subfamily I, polypeptide 1, P450-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13076
VEGA: 9
HGNC: HGNC:2595
Homologene: 68062
Nktr
Name: natural killer tumor recognition sequence
Synonyms: D9Wsu172e, 5330401F18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18087
HGNC: HGNC:7833
Homologene: 122148
Hbp1
Name: high mobility group box transcription factor 1
Synonyms: 1700058O05Rik, C330012F01Rik, C86454
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 73389
Homologene: 8171
Dock8
Name: dedicator of cytokinesis 8
Synonyms: 1200017A24Rik, 5830472H07Rik, A130095G14Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 76088
VEGA: 19
Homologene: 52414
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, 2900022L05Rik, Dnhd3, Dnahc2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Lrguk
Name: leucine-rich repeats and guanylate kinase domain containing
Synonyms: 4921528H16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74354
Homologene: 34923
Col14a1
Name: collagen, type XIV, alpha 1
Synonyms: 5730412L22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12818
HGNC: HGNC:2191
Homologene: 18741
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 214321
Homologene: 86950
Zxdc
Name: ZXD family zinc finger C
Synonyms: B930086F11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 80292
Homologene: 82340
Corin
Name: corin, serine peptidase
Synonyms: Lrp4
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53419
Homologene: 4804
Or6c212
Name: olfactory receptor family 6 subfamily C member 212
Synonyms: GA_x6K02T2PULF-11402237-11401278, MOR110-4, Olfr805
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258548
Plch2
Name: phospholipase C, eta 2
Synonyms: PLCeta2, A930027K05Rik, Plcl4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269615
Homologene: 85172
Tmprss11f
Name: transmembrane protease, serine 11f
Synonyms: 4732406D01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243083
Homologene: 65356
Or5w8
Name: olfactory receptor family 5 subfamily W member 8
Synonyms: GA_x6K02T2Q125-49358694-49359620, MOR177-9, Olfr1151
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258631
Homologene: 36999
Mmrn1
Name: multimerin 1
Synonyms: 4921530G03Rik, Emilin4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70945
HGNC: HGNC:7178
Homologene: 49134
Dpm1
Name: dolichyl-phosphate mannosyltransferase subunit 1, catalytic
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13480
HGNC: HGNC:3005
Homologene: 2865
Dip2a
Name: disco interacting protein 2 homolog A
Synonyms: Kiaa0184-hp, 4931420H10Rik, Dip2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64451
Homologene: 41012
Tcp11
Name: t-complex protein 11
Synonyms: D17Ken1, Tcp-11
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21463
Homologene: 8433
Or52ab7
Name: olfactory receptor family 52 subfamily AB member 7
Synonyms: GA_x6K02T2PBJ9-6037823-6038782, MOR23-4P, MOR23-5, Olfr598
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 257975
Homologene: 132401
Parp12
Name: poly (ADP-ribose) polymerase family, member 12
Synonyms: Zc3hdc1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243771
Homologene: 11236
Rpe65
Name: retinal pigment epithelium 65
Synonyms: A930029L06Rik, Mord1, rd12
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19892
Homologene: 20108
Or56a42-ps1
Name: olfactory receptor family 56 subfamily A member 42, pseudogene 1
Synonyms: GA_x6K02SYW8DF-188-1037, GA_x6K02T2PBJ9-7755919-7755070, MOR40-6P, MOR40-19_p, Olfr217-ps1, Olfr682-ps1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258187
Tmem71
Name: transmembrane protein 71
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 213068
VEGA: 15
Homologene: 51638
Ap5b1
Name: adaptor-related protein complex 5, beta 1 subunit
Synonyms: Gm962
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 381201
VEGA: 19
Homologene: 16299
Zfp747l1
Name: zinc finger protein 747 like 1
Synonyms: 9130019O22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 78921
Homologene: 118005
Rdh16
Name: retinol dehydrogenase 16
Synonyms: CRAD1, cis-retinol/androgen dehydrogenase 1, Rdh6
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19683
Homologene: 129514
Mroh9
Name: maestro heat-like repeat family member 9
Synonyms: 4921528O07Rik, Armc11
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78258
Homologene: 123521
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to G, chromosome 1 at 163,060,760 bp
  • G to A, chromosome 1 at 174,608,880 bp
  • A to T, chromosome 2 at 29,993,724 bp
  • A to T, chromosome 2 at 87,857,696 bp
  • C to T, chromosome 2 at 133,561,359 bp
  • C to T, chromosome 2 at 168,210,571 bp
  • T to C, chromosome 3 at 75,640,294 bp
  • T to C, chromosome 3 at 133,485,913 bp
  • A to G, chromosome 3 at 159,604,404 bp
  • T to A, chromosome 4 at 154,984,534 bp
  • A to G, chromosome 5 at 72,435,257 bp
  • T to A, chromosome 5 at 86,556,978 bp
  • T to C, chromosome 6 at 34,073,797 bp
  • A to T, chromosome 6 at 39,102,612 bp
  • A to T, chromosome 6 at 60,976,586 bp
  • T to A, chromosome 6 at 90,373,800 bp
  • G to A, chromosome 6 at 125,031,423 bp
  • T to A, chromosome 7 at 103,329,050 bp
  • C to T, chromosome 7 at 105,126,777 bp
  • G to T, chromosome 7 at 127,384,907 bp
  • A to T, chromosome 9 at 44,154,254 bp
  • A to G, chromosome 9 at 57,702,369 bp
  • A to T, chromosome 9 at 121,727,346 bp
  • T to C, chromosome 10 at 76,296,393 bp
  • G to A, chromosome 10 at 127,801,267 bp
  • T to A, chromosome 10 at 129,722,945 bp
  • C to T, chromosome 11 at 61,916,189 bp
  • T to A, chromosome 11 at 69,457,993 bp
  • T to C, chromosome 12 at 31,928,618 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • T to C, chromosome 13 at 14,625,217 bp
  • A to G, chromosome 15 at 36,600,625 bp
  • A to T, chromosome 15 at 55,338,445 bp
  • C to T, chromosome 15 at 66,555,214 bp
  • A to G, chromosome 16 at 87,354,353 bp
  • G to A, chromosome 17 at 28,067,863 bp
  • A to G, chromosome 18 at 77,962,445 bp
  • G to A, chromosome 19 at 5,569,835 bp
  • T to C, chromosome 19 at 25,163,094 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5324 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042907-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.