Strain Detail Sheet

Strain Name:
B6.Cg-Tg(OPN1LW-EGFP)85933Hue/Mmmh
Stock Number:
000043-MU
Citation ID:
RRID:MMRRC_000043-MU

Strain Information

Gene Details

OPN1LW
Name: opsin 1 (cone pigments), long-wave-sensitive (color blindness, protan)
Type: Gene
Species: Homo sapiens (human)
Chromosome: X
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Tg(OPN1LW-EGFP)85933Hue
Name: transgene insertion 85933, Thomas Hughes
Synonyms: R6.85933
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Mutant mice contain the EGFP transgene under the control of the human OPN1LW promoter which limits EGFP expression to the retina.
Genotype Determination

Strain Description

Phenotype
Many, but not all, of the cone photoreceptors contain the jellyfish green fluorescent protein. The fluorescent signal can be used to identify and record from cone photoreceptors or to purify their cell bodies by FACs.
MeSH Terms
  • Animals
  • Artificial Gene Fusion
  • Eye Proteins/genetics
  • Gene Expression
  • Green Fluorescent Proteins
  • Humans
  • Luminescent Proteins/genetics
  • Mice
  • Mice, Transgenic
  • Retinal Cone Photoreceptor Cells/physiology
  • Rod Opsins
  • Transgenes
Strain Development
DNA was microinjected into C57BL/6 x SJL F1 zygotes. Founder mice were mated to C57BL/6 mice. Founder line 85933 was continued in backcrossing for 4 generations with C57BL/6 mice during which the rd mutation of the Pde6b gene was selectively removed by continued mating of only rd negative mice. Following the removal of the rd mutation, this strain has been maintained by breeding EGFP positive carrier mice to C57BL/6 mice and then selecting EGFP transgene positive offspring for mating at the next backcross generation.
Suggested Control Mice
  • Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Eye Research
  • Macular Degeneration

Strain Origin

Donor
Thomas Hughes, Ph.D., Yale Medical School
Primary Reference
Fei Y, Hughes TE. Transgenic expression of the jellyfish green fluorescent protein in the cone photoreceptors of the mouse. Vis Neurosci. 2001 Jul-Aug;18(4):615-23. (Medline PMID: 11829307)

Colony and Husbandry Information

Special Considerations

Should not be crossed with any line that carries the rd mutation. Donor did not try to breed this transgene to homozygosity. The animals breed well. Because the founders were B6SJLF1 in origin, they carried the rd gene which causes massive loss of photoreceptors. Donor bred only rd negative animals to get rid of this.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • C57BL/6 female x Hemizygous male or reciprocal mating
Generation
N4 As of: 5/2003
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Yes Yes Note short reproductive lifespan.
Age Reproductive Decline: 4 months 4 months
Average litter size
4-5
Recommended wean age
4 weeks. These pups tend to run on the VERY small size.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
000043-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000043-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
000043-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.