Strain Name:
C57BL/6J-MtgxR5444Btlr/Mmmh
Stock Number:
043009-MU
Citation ID:
RRID:MMRRC_043009-MU
Other Names:
R5444 (G1), C57BL/6J-MtgxR5444Btlr
Major Collection:

Strain Information

Nfatc2
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1-D, NFATp, NFAT1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18019
HGNC: HGNC:7776
Homologene: 7861
Lamb1
Name: laminin B1
Synonyms: Lamb1-1, C81607, D130003D08Rik, C80098, Lamb-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Cdk17
Name: cyclin dependent kinase 17
Synonyms: 6430598J10Rik, Pctk2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237459
VEGA: 10
HGNC: HGNC:8750
Homologene: 55666
Itgb8
Name: integrin beta 8
Synonyms: 4832412O06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320910
HGNC: HGNC:6163
Homologene: 18567
Nnat
Name: neuronatin
Synonyms: 5730414I02Rik, Peg5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18111
HGNC: HGNC:7860
Homologene: 36176
Atic
Name: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Synonyms: 2610509C24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108147
HGNC: HGNC:794
Homologene: 2983
Mpc1
Name: mitochondrial pyruvate carrier 1
Synonyms: 3830411I18Rik, 0610006G08Rik, Brp44l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 55951
Homologene: 9384
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Thbs3
Name: thrombospondin 3
Synonyms: Thbs-3, TSP3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21827
Homologene: 5159
Usp46
Name: ubiquitin specific peptidase 46
Synonyms: 1190009E20Rik, 2410018I08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69727
Homologene: 56957
Nf1
Name: neurofibromin 1
Synonyms: Mhdadsk9, neurofibromin, Nf-1, Dsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Rybp
Name: RING1 and YY1 binding protein
Synonyms: 2410018J24Rik, DEDAF
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56353
Homologene: 8159
Dhdds
Name: dehydrodolichyl diphosphate synthase
Synonyms: 3222401G21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67422
Homologene: 32615
Relch
Name: RAB11 binding and LisH domain, coiled-coil and HEAT repeat containing
Synonyms: 2310035C23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227446
Homologene: 10834
Nup205
Name: nucleoporin 205
Synonyms: 3830404O05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70699
Homologene: 45971
Polr3a
Name: polymerase (RNA) III (DNA directed) polypeptide A
Synonyms: RPC1, RPC155, 9330175N20Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218832
VEGA: 14
Homologene: 5124
Tbc1d5
Name: TBC1 domain family, member 5
Synonyms: 1600014N05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72238
VEGA: 17
Homologene: 8834
Rbmxl2
Name: RNA binding motif protein, X-linked-like 2
Synonyms: 1700012H05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76572
Homologene: 113740
Asb4
Name: ankyrin repeat and SOCS box-containing 4
Synonyms: 8430401O13Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 65255
Homologene: 9392
Adam11
Name: a disintegrin and metallopeptidase domain 11
Synonyms: Mdc
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11488
HGNC: HGNC:189
Homologene: 7614
Pirb
Name: paired Ig-like receptor B
Synonyms: Gp91, Lilrb3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18733
Homologene: 134028
Chd2
Name: chromodomain helicase DNA binding protein 2
Synonyms: 5630401D06Rik, 2810013C04Rik, 2810040A01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244059
HGNC: HGNC:1917
Homologene: 37462
Cemip
Name: cell migration inducing protein, hyaluronan binding
Synonyms: Hybid, 6330404C01Rik, 12H19.01.T7, 9930013L23Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80982
Homologene: 10268
Irf7
Name: interferon regulatory factor 7
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54123
HGNC: HGNC:6122
Homologene: 128624
Ostf1
Name: osteoclast stimulating factor 1
Synonyms: C78236, SH3P2, Sh3d3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20409
HGNC: HGNC:8510
Homologene: 8227
Ncstn
Name: nicastrin
Synonyms: nicastrin, Nct, D1Dau13e, 9430068N19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 59287
Homologene: 41029
Tmub2
Name: transmembrane and ubiquitin-like domain containing 2
Synonyms: 2010008E23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72053
Homologene: 18581
Gp2
Name: glycoprotein 2 zymogen granule membrane
Synonyms: 2310037I18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67133
HGNC: HGNC:4441
Homologene: 133790
Mccc1
Name: methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
Synonyms: 2310058B18Rik, 1810045E08Rik, MCCalpha, MCCA
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72039
HGNC: HGNC:6936
Homologene: 10603
Rasgrp3
Name: RAS, guanyl releasing protein 3
Synonyms: LOC240168
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240168
VEGA: 17
Homologene: 15019
Trappc14
Name: trafficking protein particle complex 14
Synonyms: BC037034, Map11
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231807
Homologene: 10106
Bbs7
Name: Bardet-Biedl syndrome 7
Synonyms: 8430406N16Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71492
Homologene: 12395
Trank1
Name: tetratricopeptide repeat and ankyrin repeat containing 1
Synonyms: C030048J01Rik, LOC235639, A230061D21Rik, Lba1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320429
Homologene: 45845
Vmn2r25
Name: vomeronasal 2, receptor 25
Synonyms: EG545874
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 545874
Homologene: 135915
Slfn10-ps
Name: schlafen 10, pseudogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237887
Nos1ap
Name: nitric oxide synthase 1 (neuronal) adaptor protein
Synonyms: 6330408P19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70729
Homologene: 135990
Spag17
Name: sperm associated antigen 17
Synonyms: PF6, 4931427F14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74362
Homologene: 52601
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Gjc3
Name: gap junction protein, gamma 3
Synonyms: connexin 29, Cx29, Gje1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 118446
Homologene: 15399
Cyp2d26
Name: cytochrome P450, family 2, subfamily d, polypeptide 26
Synonyms: 1300006E06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76279
VEGA: 15
Homologene: 130660
B3glct
Name: beta-3-glucosyltransferase
Synonyms: LOC381694, B3galtl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381694
Homologene: 14978
Pdzrn4
Name: PDZ domain containing RING finger 4
Synonyms: 1110017D07Rik, LNX4, SAMCAP3L
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239618
VEGA: 15
Homologene: 85433
Rgs22
Name: regulator of G-protein signalling 22
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 626596
Homologene: 75047
Kcnb2
Name: potassium voltage gated channel, Shab-related subfamily, member 2
Synonyms: 9630047L19Rik, Kv2.2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98741
HGNC: HGNC:6232
Homologene: 31263
Fn3krp
Name: fructosamine 3 kinase related protein
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 238024
Homologene: 11093
Mtmr10
Name: myotubularin related protein 10
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233315
Homologene: 9822
Rnf215
Name: ring finger protein 215
Synonyms: 0610009J22Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71673
Homologene: 12402
Or10ak11
Name: olfactory receptor family 10 subfamily AK member 11
Synonyms: MOR259-11, MOR259-6, Olfr1333, GA_x6K02T2QD9B-18703033-18703986
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 258265
Homologene: 134082
Adamts17
Name: ADAM metallopeptidase with thrombospondin type 1 motif 17
Synonyms: AU023434
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233332
Homologene: 16373
Or9g3
Name: olfactory receptor family 9 subfamily G member 3
Synonyms: MOR213-6, Olfr1012, GA_x6K02T2Q125-47239120-47238185
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258561
Homologene: 64889
Sp110
Name: Sp110 nuclear body protein
Synonyms: 5830484A20Rik, Ifi75, 5031415C07Rik, 52kDa, Ipr1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109032
HGNC: HGNC:5401
Homologene: 82192
Or5b109
Name: olfactory receptor family 5 subfamily B member 109
Synonyms: Olfr1463, GA_x6K02T2RE5P-3560863-3561795, MOR202-29P
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258120
HGNC: HGNC:8324
Homologene: 133886
Alg6
Name: ALG6 alpha-1,3-glucosyltransferase
Synonyms: E230028F23Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320438
Homologene: 6920
Or52k2
Name: olfactory receptor family 52 subfamily K member 2
Synonyms: MOR28-1, GA_x6K02T2PBJ9-5323062-5324015, Olfr552
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259106
Homologene: 73941
Pnlip
Name: pancreatic lipase
Synonyms: 1810007A24Rik, PTL, pancreatic triglyceride lipase
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 69060
VEGA: 19
HGNC: HGNC:9155
Homologene: 30999
Or3a4
Name: olfactory receptor family 3 subfamily A member 4
Synonyms: Olfr399, GA_x6K02T2P1NL-4211516-4210581, MOR255-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 259006
Homologene: 27325
4930584F24Rik
Name: RIKEN cDNA 4930584F24 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 75942
Neurl3
Name: neuralized E3 ubiquitin protein ligase 3
Synonyms: Lincr, 2010300P06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 214854
Homologene: 35002
Sult2a1
Name: sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
Synonyms: Std, Sth1, mSTa1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20859
Homologene: 37741
Eef2kmt
Name: eukaryotic elongation factor 2 lysine methyltransferase
Synonyms: 5730409G15Rik, Fam86
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70511
Homologene: 45719
Apol9b
Name: apolipoprotein L 9b
Synonyms: 2310016F22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 71898
VEGA: 15
Homologene: 77567
Sgo2b
Name: shugoshin 2B
Synonyms: Gm4975, Sgol2b
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244495
Homologene: 51867
Gm15856
Name: predicted gene 15856
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
1700066N21Rik
Name: RIKEN cDNA 1700066N21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73471
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 15,711,492 bp
  • G to T, chromosome 1 at 36,269,490 bp
  • T to C, chromosome 1 at 71,576,717 bp
  • T to A, chromosome 1 at 85,589,148 bp
  • C to T, chromosome 1 at 105,726,384 bp
  • T to C, chromosome 1 at 170,375,251 bp
  • A to C, chromosome 1 at 172,072,839 bp
  • A to T, chromosome 2 at 85,759,919 bp
  • T to C, chromosome 2 at 157,561,217 bp
  • A to G, chromosome 2 at 168,534,890 bp
  • T to A, chromosome 3 at 35,976,742 bp
  • T to C, chromosome 3 at 36,612,050 bp
  • T to C, chromosome 3 at 89,223,385 bp
  • T to C, chromosome 3 at 100,056,152 bp
  • A to G, chromosome 4 at 99,741,579 bp
  • T to A, chromosome 4 at 118,830,111 bp
  • G to A, chromosome 4 at 133,971,136 bp
  • A to G, chromosome 5 at 26,479,737 bp
  • A to G, chromosome 5 at 74,016,731 bp
  • T to C, chromosome 5 at 87,979,288 bp
  • A to T, chromosome 5 at 109,675,636 bp
  • G to A, chromosome 5 at 137,957,547 bp
  • A to T, chromosome 5 at 138,260,998 bp
  • C to T, chromosome 5 at 149,746,520 bp
  • T to A, chromosome 6 at 5,431,040 bp
  • T to A, chromosome 6 at 35,189,189 bp
  • A to T, chromosome 6 at 100,287,270 bp
  • A to T, chromosome 6 at 121,226,101 bp
  • T to C, chromosome 6 at 123,828,492 bp
  • A to T, chromosome 7 at 3,712,750 bp
  • A to T, chromosome 7 at 13,836,019 bp
  • T to C, chromosome 7 at 64,288,401 bp
  • T to A, chromosome 7 at 67,041,899 bp
  • T to C, chromosome 7 at 73,473,085 bp
  • T to A, chromosome 7 at 83,982,291 bp
  • C to T, chromosome 7 at 102,604,869 bp
  • G to C, chromosome 7 at 107,209,837 bp
  • G to A, chromosome 7 at 119,454,598 bp
  • C to T, chromosome 7 at 141,264,819 bp
  • T to C, chromosome 8 at 63,926,556 bp
  • A to G, chromosome 9 at 109,106,453 bp
  • T to A, chromosome 9 at 111,392,958 bp
  • T to G, chromosome 10 at 93,217,961 bp
  • A to G, chromosome 11 at 4,135,843 bp
  • T to C, chromosome 11 at 74,053,977 bp
  • T to C, chromosome 11 at 79,443,959 bp
  • C to T, chromosome 11 at 83,035,287 bp
  • T to C, chromosome 11 at 102,288,240 bp
  • A to G, chromosome 11 at 102,772,848 bp
  • G to A, chromosome 11 at 121,421,604 bp
  • T to C, chromosome 12 at 31,298,909 bp
  • T to A, chromosome 12 at 111,838,688 bp
  • T to C, chromosome 12 at 119,237,838 bp
  • T to C, chromosome 14 at 24,454,941 bp
  • T to C, chromosome 15 at 36,015,627 bp
  • T to C, chromosome 15 at 77,735,763 bp
  • G to T, chromosome 15 at 82,792,538 bp
  • T to A, chromosome 15 at 92,770,925 bp
  • G to A, chromosome 16 at 5,249,095 bp
  • A to G, chromosome 17 at 8,296,508 bp
  • A to G, chromosome 17 at 50,735,967 bp
  • A to T, chromosome 17 at 75,503,375 bp
  • A to T, chromosome 19 at 13,234,958 bp
  • A to G, chromosome 19 at 18,581,313 bp
  • A to G, chromosome 19 at 58,673,163 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5444 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043009-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.