Strain Name:
C57BL/6J-MtgxR5531Btlr/Mmmh
Stock Number:
043089-MU
Citation ID:
RRID:MMRRC_043089-MU
Other Names:
R5531 (G1), C57BL/6J-MtgxR5531Btlr
Major Collection:

Strain Information

Epha4
Name: Eph receptor A4
Synonyms: Cek8, Sek, Tyro1, Hek8, 2900005C20Rik, Sek1, rb
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Jade1
Name: jade family PHD finger 1
Synonyms: Phf17, D530048A03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269424
Homologene: 18162
Igf2bp2
Name: insulin-like growth factor 2 mRNA binding protein 2
Synonyms: C330012H03Rik, IMP2, IMP-2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 319765
Homologene: 4774
Mcm3
Name: minichromosome maintenance complex component 3
Synonyms: p1.m, Mcmd, P1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17215
HGNC: HGNC:6945
Homologene: 1791
Bard1
Name: BRCA1 associated RING domain 1
Synonyms: ENSMUSG00000060893, ENSMUSG00000073653
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12021
HGNC: HGNC:952
Homologene: 400
Dhx40
Name: DEAH-box helicase 40
Synonyms: ARG147, DDX40, 2410016C14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67487
Homologene: 57000
Zbtb20
Name: zinc finger and BTB domain containing 20
Synonyms: A930017C21Rik, 7330412A13Rik, HOF, D16Wsu73e, 1300017A20Rik, Zfp288
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 56490
Homologene: 9226
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: Dnahc7, LOC381341, Dnahc7a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 627872
Homologene: 41287
Zswim6
Name: zinc finger SWIM-type containing 6
Synonyms: 2900036G02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 67263
VEGA: 13
Homologene: 83517
Lca5
Name: Leber congenital amaurosis 5 (human)
Synonyms: 4930431B11Rik, 5730406O13Rik, ORF64
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 75782
Homologene: 32718
Ap5z1
Name: adaptor-related protein complex 5, zeta 1 subunit
Synonyms: C330006K01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231855
Homologene: 18213
Il2ra
Name: interleukin 2 receptor, alpha chain
Synonyms: Ly-43, Il2r, IL-2R alpha chain, CD25
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16184
HGNC: HGNC:6008
Homologene: 360
Smurf2
Name: SMAD specific E3 ubiquitin protein ligase 2
Synonyms: 2810411E22Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66313
Homologene: 41490
Ino80
Name: INO80 complex subunit
Synonyms: 2310079N15Rik, INO80, Inoc1, 4632409L19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68142
Homologene: 75070
Zfyve19
Name: zinc finger, FYVE domain containing 19
Synonyms: 1500041L05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72008
Homologene: 12435
Tubgcp2
Name: tubulin, gamma complex component 2
Synonyms: 1700022B05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74237
Homologene: 55980
Hibch
Name: 3-hydroxyisobutyryl-Coenzyme A hydrolase
Synonyms: HIBYL-COA-H, 2610509I15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227095
HGNC: HGNC:4908
Homologene: 5607
Cwc22
Name: CWC22 spliceosome-associated protein
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 80744
Homologene: 6048
Stk32b
Name: serine/threonine kinase 32B
Synonyms: YANK2, Stk32, STKG6, 2510009F08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 64293
Homologene: 48654
Siglech
Name: sialic acid binding Ig-like lectin H
Synonyms: Siglec-H, 6430529G09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233274
Homologene: 48041
Spp1
Name: secreted phosphoprotein 1
Synonyms: bone sialoprotein 1, Spp-1, 44kDa bone phosphoprotein, 2ar, osteopontin, minopontin, Ric, Opn, BNSP, ETA-1, OP, osteopontin-like protein, Apl-1, Opnl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20750
Homologene: 20156
Acr
Name: acrosin prepropeptide
Synonyms: preproacrosin
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11434
VEGA: 15
HGNC: HGNC:126
Homologene: 855
Ncoa1
Name: nuclear receptor coactivator 1
Synonyms: KAT13A, bHLHe74, SRC-1, SRC-a/NCoA-1, steroid receptor coactivator-1, SRC1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 17977
VEGA: 12
HGNC: HGNC:7668
Homologene: 7859
Cubn
Name: cubilin
Synonyms: D2Wsu88e, intrinsic factor-cobalamin receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Prkg2
Name: protein kinase, cGMP-dependent, type II
Synonyms: cGK-II, Prkgr2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19092
HGNC: HGNC:9416
Homologene: 4556
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 545370
Homologene: 23741
Ptprd
Name: protein tyrosine phosphatase receptor type D
Synonyms: 3000002J10Rik, 1110002J03Rik, B230219D21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19266
HGNC: HGNC:9668
Acacb
Name: acetyl-Coenzyme A carboxylase beta
Synonyms: Accb, Acc2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100705
HGNC: HGNC:85
Homologene: 74382
Wdr11
Name: WD repeat domain 11
Synonyms: 2900055P10Rik, Brwd2, Wdr11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 207425
Homologene: 41229
Clspn
Name: claspin
Synonyms: E130314M08Rik, C85083
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269582
Homologene: 11138
Xirp2
Name: xin actin-binding repeat containing 2
Synonyms: mXin beta, myomaxin, 2310003D02Rik, Cmya3, 2310008C07Rik, A530024P18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241431
Homologene: 19388
Brd10
Name: bromodomain containing 10
Synonyms: Gm9832, 9930021J03Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240613
Homologene: 19046
Stxbp5
Name: syntaxin binding protein 5 (tomosyn)
Synonyms: 4930565N16Rik, LGL3, 0710001E20Rik, tomosyn 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 78808
Homologene: 16402
Scap
Name: SREBF chaperone
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235623
Homologene: 8160
Gucy2g
Name: guanylate cyclase 2g
Synonyms: 2410077I05Rik, GC-G
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 73707
Homologene: 44544
Ly75
Name: lymphocyte antigen 75
Synonyms: DEC-205, CD205
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17076
Homologene: 31085
Tulp4
Name: TUB like protein 4
Synonyms: 2210038L17Rik, 1110057P05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 68842
Homologene: 32467
Fbxw10
Name: F-box and WD-40 domain protein 10
Synonyms: SM2SH2, SM25H2, Fbw10
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 213980
Homologene: 32757
Or5p5
Name: olfactory receptor family 5 subfamily P member 5
Synonyms: MOR204-33P, GA_x6K02T2PBJ9-10144091-10145011, Olfr467
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 257919
Homologene: 81542
Ankrd42
Name: ankyrin repeat domain 42
Synonyms: 4933417L02Rik, Ikbn
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73845
Homologene: 23652
Hsd11b1
Name: hydroxysteroid 11-beta dehydrogenase 1
Synonyms: 11beta-hydroxysteroid dehydrogenase type 1, 11beta-HSD-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 15483
HGNC: HGNC:5208
Homologene: 68471
G930045G22Rik
Name: RIKEN cDNA G930045G22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Ncf4
Name: neutrophil cytosolic factor 4
Synonyms: p40phox
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17972
HGNC: HGNC:7662
Homologene: 525
Or6c8b
Name: olfactory receptor family 6 subfamily C member 8B
Synonyms: GA_x6K02T2PULF-10732607-10731678, Olfr765, MOR115-4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 544748
Homologene: 105186
Sgk2
Name: serum/glucocorticoid regulated kinase 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27219
Homologene: 8446
Slc40a1
Name: solute carrier family 40 (iron-regulated transporter), member 1
Synonyms: Dusg, IREG1, FPN1, ferroportin1, Pcm, Slc11a3, metal transporting protein 1, Ol5, MTP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 53945
Homologene: 40959
Sv2c
Name: synaptic vesicle glycoprotein 2c
Synonyms: 4930527L09Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75209
Homologene: 57152
Ankrd29
Name: ankyrin repeat domain 29
Synonyms: G630054C21Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225187
Homologene: 17839
Vmn2r76
Name: vomeronasal 2, receptor 76
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 675969
Homologene: 104330
Lsp1
Name: lymphocyte specific 1
Synonyms: Lsp-1, lymphocyte-specific protein 1, leukocyte-specific protein 1, p50, pp52, leukocyte specific protein 1, WP34
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16985
HGNC: HGNC:6707
Homologene: 1750
Sgpp1
Name: sphingosine-1-phosphate phosphatase 1
Synonyms: SPP, SPP1, mSPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 81535
VEGA: 12
Homologene: 101696
Zfp957
Name: zinc finger protein 957
Synonyms: AU017455
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105590
Gpr156
Name: G protein-coupled receptor 156
Synonyms: Gababl
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239845
VEGA: 16
Homologene: 17683
AI987944
Name: expressed sequence AI987944
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233168
Or1e16
Name: olfactory receptor family 1 subfamily E member 16
Synonyms: MOR135-13, GA_x6K02T2P1NL-3556334-3555390, I54, Olfr1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258923
Homologene: 115482
Speer4f2
Name: spermatogenesis associated glutamate (E)-rich protein 4f2
Synonyms: Gm3495, Gm3535
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100041749
Ifi214
Name: interferon activated gene 214
Synonyms: BC094916, Pyhin-B
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 545384
HGNC: HGNC:5395
Homologene: 115929
Ubap1l
Name: ubiquitin-associated protein 1-like
Synonyms: Gm514, LOC208080
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 208080
VEGA: 9
Homologene: 19477
Lce1l
Name: late cornified envelope 1L
Synonyms: 1110008K04Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 73730
Dact3
Name: dishevelled-binding antagonist of beta-catenin 3
Synonyms: EG666671
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 629378
Homologene: 77340
Rps15a-ps5
Name: ribosomal protein S15A, pseudogene 5
Synonyms: Gm10196
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100042335
Homologene: 134982
Gm21103
Name: predicted gene, 21103
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 118567363
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,803,544 bp
  • A to G, chromosome 1 at 45,912,338 bp
  • A to G, chromosome 1 at 52,845,069 bp
  • A to G, chromosome 1 at 53,419,748 bp
  • A to C, chromosome 1 at 71,046,721 bp
  • A to G, chromosome 1 at 77,374,876 bp
  • A to T, chromosome 1 at 150,743,788 bp
  • A to G, chromosome 1 at 173,525,120 bp
  • CGG to CG, chromosome 1 at 193,240,249 bp
  • A to T, chromosome 2 at 11,676,892 bp
  • C to T, chromosome 2 at 13,350,932 bp
  • T to C, chromosome 2 at 60,365,145 bp
  • C to G, chromosome 2 at 67,515,302 bp
  • T to C, chromosome 2 at 77,924,569 bp
  • C to T, chromosome 2 at 119,211,946 bp
  • T to A, chromosome 2 at 119,445,575 bp
  • T to C, chromosome 2 at 162,994,704 bp
  • A to C, chromosome 3 at 41,613,511 bp
  • G to T, chromosome 3 at 92,850,497 bp
  • A to C, chromosome 4 at 76,059,667 bp
  • A to G, chromosome 4 at 126,577,773 bp
  • A to G, chromosome 5 at 17,376,528 bp
  • T to C, chromosome 5 at 37,459,734 bp
  • T to C, chromosome 5 at 98,967,734 bp
  • T to G, chromosome 5 at 104,440,558 bp
  • T to C, chromosome 5 at 105,797,670 bp
  • T to C, chromosome 5 at 114,204,706 bp
  • T to C, chromosome 5 at 142,467,781 bp
  • C to A, chromosome 6 at 50,847,776 bp
  • A to G, chromosome 7 at 16,875,615 bp
  • A to T, chromosome 7 at 41,374,390 bp
  • A to T, chromosome 7 at 55,768,665 bp
  • A to T, chromosome 7 at 86,225,449 bp
  • G to A, chromosome 7 at 92,612,828 bp
  • A to G, chromosome 7 at 107,815,244 bp
  • T to C, chromosome 7 at 129,606,524 bp
  • A to C, chromosome 7 at 140,005,024 bp
  • A to T, chromosome 7 at 142,490,621 bp
  • C to T, chromosome 9 at 65,371,691 bp
  • C to T, chromosome 9 at 83,398,595 bp
  • A to G, chromosome 9 at 110,381,429 bp
  • G to A, chromosome 10 at 9,762,924 bp
  • G to C, chromosome 10 at 129,046,564 bp
  • G to A, chromosome 11 at 62,862,656 bp
  • T to C, chromosome 11 at 73,395,177 bp
  • T to C, chromosome 11 at 86,789,504 bp
  • G to A, chromosome 11 at 106,852,563 bp
  • T to A, chromosome 12 at 4,253,746 bp
  • G to T, chromosome 12 at 75,735,207 bp
  • T to A, chromosome 13 at 95,961,378 bp
  • T to C, chromosome 13 at 107,769,593 bp
  • A to T, chromosome 14 at 6,303,861 bp
  • T to A, chromosome 14 at 79,213,182 bp
  • T to G, chromosome 15 at 78,260,788 bp
  • T to A, chromosome 15 at 89,573,943 bp
  • T to G, chromosome 16 at 22,089,085 bp
  • T to C, chromosome 16 at 38,005,257 bp
  • C to A, chromosome 16 at 43,610,867 bp
  • G to T, chromosome 17 at 6,214,640 bp
  • G to A, chromosome 18 at 12,279,778 bp
  • A to G, chromosome 19 at 29,753,672 bp
  • A to G, chromosome 19 at 55,241,140 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5531 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043089-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.