Strain Name:
C57BL/6J-MtgxR5538Btlr/Mmmh
Stock Number:
043096-MU
Citation ID:
RRID:MMRRC_043096-MU
Other Names:
R5538 (G1), C57BL/6J-MtgxR5538Btlr
Major Collection:

Strain Information

Dnm2
Name: dynamin 2
Synonyms: Dyn2, b2b2159Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13430
HGNC: HGNC:2974
Homologene: 90883
Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Slc1a3
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: MGluT1, Eaat1, B430115D02Rik, GLAST, Gmt1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20512
Homologene: 20882
Klf2
Name: Kruppel-like transcription factor 2 (lung)
Synonyms: Lklf
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16598
HGNC: HGNC:6347
Homologene: 133978
Clasrp
Name: CLK4-associating serine/arginine rich protein
Synonyms: SWAP2, Sfrs16, Srsf16
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 53609
Homologene: 134306
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: Ese1, EHSH1, Sh3p17, Eh domain, SH3 domain regulator of endocytosis 1, Intersectin-L
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Arl8b
Name: ADP-ribosylation factor-like 8B
Synonyms: 3100002J04Rik, 2610313E07Rik, Arl10c
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67166
Homologene: 10056
Stk11ip
Name: serine/threonine kinase 11 interacting protein
Synonyms: LKB1IP, LIP1, 1200014D22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71728
Homologene: 12406
Hells
Name: helicase, lymphoid specific
Synonyms: Lysh, proliferation-associated SNF2-like, PASG, LSH, YFK8, E130115I21Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15201
HGNC: HGNC:4861
Homologene: 50037
Usp32
Name: ubiquitin specific peptidase 32
Synonyms: 6430526O11Rik, 2900074J03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237898
Homologene: 13066
Kif20b
Name: kinesin family member 20B
Synonyms: N-6 kinesin, C330014J10Rik, Kif20b, Mphosph1, 33cex, magoo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240641
VEGA: 19
HGNC: HGNC:7212
Homologene: 9418
Fry
Name: FRY microtubule binding protein
Synonyms: cg003, 9330186A19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320365
Homologene: 113770
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: trithorax Drosophila, HTRX1, ALL-1, All1, Cxxc7, Mll, Mll1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Phkb
Name: phosphorylase kinase beta
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102093
HGNC: HGNC:8927
Homologene: 247
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, Ankyrin-3, AnkG, 2900054D09Rik, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Cc2d2a
Name: coiled-coil and C2 domain containing 2A
Synonyms: 5730509K17Rik, b2b1035Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231214
Homologene: 18159
C2cd3
Name: C2 calcium-dependent domain containing 3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 277939
Homologene: 19524
Npnt
Name: nephronectin
Synonyms: POEM, 1110009H02Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 114249
Homologene: 14138
C6
Name: complement component 6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12274
HGNC: HGNC:1339
Homologene: 47
Dhx32
Name: DEAH-box helicase 32 (putative)
Synonyms: Ddx32, DEAH (Asp-Glu-Ala-His) box polypeptide 32
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101437
Homologene: 56798
Ern1
Name: endoplasmic reticulum to nucleus signalling 1
Synonyms: 9030414B18Rik, Ire1p, Ire1alpha, Ire1a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78943
HGNC: HGNC:3449
Homologene: 55580
Ror1
Name: receptor tyrosine kinase-like orphan receptor 1
Synonyms: Ntrkr1, 2810404D04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26563
Homologene: 3675
Cep63
Name: centrosomal protein 63
Synonyms: CD20R, ET2, D9Mgc41, D9Mgc48e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 28135
Homologene: 11861
Jak3
Name: Janus kinase 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16453
HGNC: HGNC:6193
Homologene: 181
Gramd1c
Name: GRAM domain containing 1C
Synonyms: 4921521N14Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207798
Homologene: 129735
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: DHC1b, DHC2, 4432416O06Rik, D330044F14Rik, D030010H02Rik, Dnchc2, b2b414Clo, m407Asp, m152Asp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Pnpla6
Name: patatin-like phospholipase domain containing 6
Synonyms: Swiss-cheese, MSws, Nte
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50767
Homologene: 21333
Lonrf1
Name: LON peptidase N-terminal domain and ring finger 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244421
Homologene: 34980
Cox16
Name: cytochrome c oxidase assembly protein 16
Synonyms: 1810055I05Rik, 1810020G14Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66272
Homologene: 9520
Htr7
Name: 5-hydroxytryptamine (serotonin) receptor 7
Synonyms: 5-HT7
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15566
HGNC: HGNC:5302
Homologene: 20244
Flt3l
Name: FMS-like tyrosine kinase 3 ligand
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14256
HGNC: HGNC:3766
Homologene: 48021
Col24a1
Name: collagen, type XXIV, alpha 1
Synonyms: 5430404K19Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71355
Homologene: 65061
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: 4833413O10Rik, D430029O09Rik, 1110021D17Rik, Polydom
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 64817
Homologene: 23386
Trappc14
Name: trafficking protein particle complex 14
Synonyms: Map11, BC037034
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231807
Homologene: 10106
Dpysl4
Name: dihydropyrimidinase-like 4
Synonyms: unc-33-like phosphoprotein 4, Ulip4, CRMP-3, Drp-4, DPY4, Crmp3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26757
HGNC: HGNC:3016
Homologene: 4691
Vmn2r116
Name: vomeronasal 2, receptor 116
Synonyms: EG619697, V2Rp5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 619697
Homologene: 86604
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mass1, Mgr1, Gpr98
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110789
Homologene: 19815
Fbn2
Name: fibrillin 2
Synonyms: sy, Sne, Fib-2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Tie1
Name: tyrosine kinase with immunoglobulin-like and EGF-like domains 1
Synonyms: TIE, D430008P04Rik, tie-1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21846
Homologene: 3957
Pcnx1
Name: pecanex 1
Synonyms: 3526401J03Rik, 2900024E21Rik, Pcnx
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 54604
VEGA: 12
Homologene: 40997
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243369
Homologene: 45453
Fmo9
Name: flavin containing monooxygenase 9
Synonyms: 4831428F09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240894
Homologene: 134192
Kmt2d
Name: lysine (K)-specific methyltransferase 2D
Synonyms: C430014K11Rik, Mll4, Mll2, bapa
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 381022
HGNC: HGNC:7133
Homologene: 86893
Txlnb
Name: taxilin beta
Synonyms: Mdp77, 2310001N14Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 378431
Homologene: 44534
Mybpc1
Name: myosin binding protein C, slow-type
Synonyms: Slow-type C-protein, 8030451F13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 109272
HGNC: HGNC:7549
Homologene: 1846
Sytl2
Name: synaptotagmin-like 2
Synonyms: Slp2-a, Slp2, Slp2-d, Slp2-c, Slp2-b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 83671
Homologene: 131343
Or6n1
Name: olfactory receptor family 6 subfamily N member 1
Synonyms: GA_x6K02T2P20D-21090094-21089156, MOR105-1, Olfr429
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 258717
Homologene: 17366
Cd46
Name: CD46 antigen, complement regulatory protein
Synonyms: CD46, Mcp
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17221
HGNC: HGNC:6953
Homologene: 7832
Pycr1
Name: pyrroline-5-carboxylate reductase 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 209027
HGNC: HGNC:9721
Homologene: 56002
Tle2
Name: transducin-like enhancer of split 2
Synonyms: Grg2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21886
Homologene: 20693
H2-Bl
Name: histocompatibility 2, blastocyst
Synonyms: blastocyst MHC
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14963
Homologene: 128352
Or6c69
Name: olfactory receptor family 6 subfamily C member 69
Synonyms: GA_x6K02T2PULF-11590830-11589892, MOR113-1, Olfr816
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258667
Homologene: 133584
Ceacam3
Name: CEA cell adhesion molecule 3
Synonyms: cea12, EG384557, Psg24
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384557
HGNC: HGNC:1819
Homologene: 86971
Fez1
Name: fasciculation and elongation protein zeta 1
Synonyms: UNC-76, UNC76, zygin I
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235180
HGNC: HGNC:3659
Homologene: 21063
Slc19a3
Name: solute carrier family 19, member 3
Synonyms: ThTr2, A230084E24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 80721
Homologene: 23530
Arhgap11a
Name: Rho GTPase activating protein 11A
Synonyms: GAP (1-12), 6530401L14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228482
Homologene: 8862
Skint11
Name: selection and upkeep of intraepithelial T cells 11
Synonyms: A630098G03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230623
Homologene: 136292
Scnm1
Name: sodium channel modifier 1
Synonyms: 3110001I17Rik, Scnm1-ps
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69269
Homologene: 11431
Gnpda2
Name: glucosamine-6-phosphate deaminase 2
Synonyms: 4921523I18Rik, Gnp2, 4933412A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67980
Homologene: 12381
Or51h5
Name: olfactory receptor family 51 subfamily H member 5
Synonyms: GA_x6K02T2PBJ9-5638785-5639741, MOR10-2, Olfr572
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259093
Homologene: 131142
Upk3b
Name: uroplakin 3B
Synonyms: PMS2L14, UpIIIb, P35
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100647
Homologene: 18435
Or4c12b
Name: olfactory receptor family 4 subfamily C member 12B
Synonyms: GA_x6K02T2Q125-51257221-51258135, MOR232-4, Olfr1255
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258979
Homologene: 82298
Cybb
Name: cytochrome b-245, beta polypeptide
Synonyms: gp91phox, gp91phox, Nox2, Cgd
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 13058
HGNC: HGNC:2578
Homologene: 68054
Bbs2
Name: Bardet-Biedl syndrome 2
Synonyms: 2410125H22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67378
HGNC: HGNC:967
Homologene: 12122
Zfp607b
Name: zinc finger protein 607B
Synonyms: C030039L03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 112415
Homologene: 134321
Fam221b
Name: family with sequence similarity 221, member B
Synonyms: 4930412F15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242408
Homologene: 52184
Smok2b
Name: sperm motility kinase 2B
Synonyms: LOC236574
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 236574
Homologene: 128738
Clk2
Name: CDC-like kinase 2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12748
HGNC: HGNC:2069
Homologene: 33303
Gm10719
Name: predicted gene 10719
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
2900055J20Rik
Name: RIKEN cDNA 2900055J20 gene
Type: Gene
Species: Mouse
Chromosome: 18
Igkv13-54-1
Name: immunoglobulin kappa chain variable 13-54-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 692105
Gm4535
Name: predicted gene 4535
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043585
Potefam3e
Name: POTE ankyrin domain family member 3E
Synonyms: ENSMUSG00000074453, Pote3e, TSAP, OTTMUSG00000018948, 4930467E23Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 626415
Homologene: 128726
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 75,528,335 bp
  • T to A, chromosome 1 at 83,022,561 bp
  • T to A, chromosome 1 at 166,673,629 bp
  • T to C, chromosome 1 at 174,089,978 bp
  • T to G, chromosome 1 at 195,068,170 bp
  • A to T, chromosome 2 at 40,697,474 bp
  • T to A, chromosome 2 at 89,816,620 bp
  • G to T, chromosome 2 at 113,837,530 bp
  • T to A, chromosome 3 at 89,175,655 bp
  • A to G, chromosome 3 at 95,129,755 bp
  • T to C, chromosome 3 at 132,904,963 bp
  • C to T, chromosome 3 at 145,293,121 bp
  • C to A, chromosome 4 at 43,664,158 bp
  • T to C, chromosome 4 at 58,049,282 bp
  • T to C, chromosome 4 at 100,441,011 bp
  • A to T, chromosome 4 at 114,231,762 bp
  • T to C, chromosome 4 at 118,486,193 bp
  • T to C, chromosome 5 at 43,695,176 bp
  • A to T, chromosome 5 at 69,578,051 bp
  • A to T, chromosome 5 at 104,175,230 bp
  • C to G, chromosome 5 at 136,044,036 bp
  • C to T, chromosome 5 at 138,262,695 bp
  • T to A, chromosome 5 at 150,495,848 bp
  • T to C, chromosome 6 at 48,452,178 bp
  • T to A, chromosome 6 at 69,617,263 bp
  • C to A, chromosome 6 at 108,783,336 bp
  • A to T, chromosome 7 at 17,158,421 bp
  • A to C, chromosome 7 at 19,584,782 bp
  • A to G, chromosome 7 at 27,702,869 bp
  • A to G, chromosome 7 at 45,132,348 bp
  • A to G, chromosome 7 at 90,388,906 bp
  • T to A, chromosome 7 at 100,455,493 bp
  • A to G, chromosome 7 at 102,928,521 bp
  • A to T, chromosome 7 at 133,723,217 bp
  • A to G, chromosome 7 at 139,091,990 bp
  • A to G, chromosome 7 at 141,406,845 bp
  • A to G, chromosome 8 at 3,531,508 bp
  • A to C, chromosome 8 at 19,749,414 bp
  • T to A, chromosome 8 at 36,223,024 bp
  • A to G, chromosome 8 at 71,678,773 bp
  • T to C, chromosome 8 at 72,319,472 bp
  • G to A, chromosome 8 at 85,922,127 bp
  • G to A, chromosome 8 at 94,089,763 bp
  • T to C, chromosome 9 at 3,018,962 bp
  • T to C, chromosome 9 at 7,168,630 bp
  • T to C, chromosome 9 at 21,505,627 bp
  • T to A, chromosome 9 at 36,868,876 bp
  • A to G, chromosome 9 at 44,820,342 bp
  • A to T, chromosome 9 at 102,588,793 bp
  • T to C, chromosome 10 at 17,838,909 bp
  • A to G, chromosome 10 at 69,987,427 bp
  • T to C, chromosome 10 at 81,580,584 bp
  • T to A, chromosome 10 at 88,546,029 bp
  • T to A, chromosome 10 at 129,912,002 bp
  • A to T, chromosome 11 at 85,017,786 bp
  • A to G, chromosome 11 at 106,421,901 bp
  • T to C, chromosome 11 at 120,641,710 bp
  • T to C, chromosome 12 at 81,484,929 bp
  • T to C, chromosome 12 at 81,860,409 bp
  • C to A, chromosome 13 at 81,433,689 bp
  • T to A, chromosome 15 at 4,814,829 bp
  • A to T, chromosome 15 at 8,645,704 bp
  • G to A, chromosome 15 at 98,852,109 bp
  • A to G, chromosome 16 at 15,651,469 bp
  • T to A, chromosome 16 at 43,982,092 bp
  • C to A, chromosome 16 at 91,784,102 bp
  • T to A, chromosome 17 at 13,235,553 bp
  • C to T, chromosome 17 at 23,401,067 bp
  • T to A, chromosome 17 at 36,081,286 bp
  • T to A, chromosome 18 at 40,257,266 bp
  • G to A, chromosome 18 at 58,071,901 bp
  • A to T, chromosome 19 at 34,952,964 bp
  • A to G, chromosome 19 at 35,969,835 bp
  • T to A, chromosome 19 at 38,953,652 bp
  • C to G, chromosome X at 9,450,750 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5538 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043096-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.