Strain Name:
B6J.129-Dlx1tm3.1Jlr/Mmucd
Stock Number:
044028-UCD
Citation ID:
RRID:MMRRC_044028-UCD
Other Names:
Dlx1-ki-FLAG-eGFP

Strain Information

Dlx1tm3.1Jlr
Name: distal-less homeobox 1; targeted mutation 3.1, John Rubenstein
Synonyms: Dlx1-ki-FLAG-eGFP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knock-In
Dlx1
Name: distal-less homeobox 1
Synonyms: DII B, Dlx-1, Dlx
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Knock-In
NCBI: 13390
HGNC: HGNC:2914
Homologene: 22558
Genetic Alterations
This is a knockin into the Dlx1 locus of a modification of Dlx1 (Flag tagged C-terminus) followed by an IRES and eGFP. There is a single loxP sequence in intron 2, that is residual to having a neo cassette in this location that was removed by Cre.
Genotype Determination
ES Cell Line
The ES cell line was derived from an F1(C57BL/6J x 129/Sv) embryo.
Phenotype

EGFP is correctly expressed by the Dlx1 locus in the developing basal ganglia, cortical interneurons, and in the head. Otherwise no phenotype detected in the heterozygotes or homozygotes.

Strain Development
A triple-FLAG tag (Dlx1-3x-FLAG) was appended to the C-terminus of the DLX1 protein. Transfection activation experiments in tissue culture cells, using an expression vector encoding the Dlx1-3x-FLAG c-terminus, showed that this form of the DLX1 protein’s transcriptional activation properties was indistinguishable with wild-type Dlx1. Chimeric animals were bred with Hprt-Cre (backcrossed over 30 generations with C57BL/6J to remove the Neo cassette). The strain background of the F1 mice generated was 75% B6J / 25% 129Sv. Once the mice arrived at UCSF the line was crossed with C57BL/6J for roughly 5 generations, and then the heterozygotes were inbred to generate homozygotes. Prior to its arrival at the MMRRC, the line was maintained by inbreeding the homozygotes.
Suggested Control Mice
wild-type C57BL/6J
MMRRC Genetic QC Summary
Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary. The impact of genetic background on previously reported phenotypes has not been evaluated.

GQC analysis was done on one XY sample, IE7920, at the time of importation. The results of the analysis are inconsistent with the SDS, see details below. The primary (host) background is C57BL/6. There is positive evidence of C57BL/6J and C57BL/6N substrains. The secondary (donor) background, 129Sv, is consistent with the F1(C57BL/6J x 129/Sv) ES cells indicated in the submission and the SDS and 129S6SvEv diagnostics are detected. However, there is an additional unknown strain present. The mitochondrial genome is consistent with at least one backcross to a female from the C57BL/6 background. The Y chromosome is consistent with at least one backcross to a male from the host/C57BL6J background. The sample is homozygous for the donor background at the locus. IRES and g_FP constructs are detected in the sample.

This sample has more than 2 genetic backgrounds (unexplained regions and/or fractured ideogram). The strain selected for secondary background may be incorrect. The estimation of the contribution of primary and secondary background are likely incorrect.
MiniMUGA Sample Report
  • Developmental Biology
  • Neurobiology
Donor
John Rubenstein, M.D., University of California, San Francisco.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
The MMRRC at UC Davis performed microbita analysis on feces samples received from the donating investigator while the mice were still at their facility. There is a MMRRC Fecal Microbiota report available for this strain.If you are interested in having the MMRRC at UC Davis perform microbiota analysis once the line is established at your facility please contact mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
N=5 (C57BL/6J)
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 6 to 8 months
Bred to Homozygosity
Yes
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044028-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
044028-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.