Strain Name:
C57BL/6J-MtgxR6446Btlr/Mmmh
Stock Number:
044583-MU
Citation ID:
RRID:MMRRC_044583-MU
Other Names:
R6446 (G1)
Major Collection:

Strain Information

Map7
Name: microtubule-associated protein 7
Synonyms: ste, Mtap7, mste, mshi, E-MAP-115
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17761
VEGA: 10
HGNC: HGNC:6869
Homologene: 20851
Blm
Name: Bloom syndrome, RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12144
HGNC: HGNC:1058
Homologene: 47902
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77595
Homologene: 28122
Slc29a1
Name: solute carrier family 29 (nucleoside transporters), member 1
Synonyms: ENT1, 1200014D21Rik, NBMPR-sensitive equilibrative nucleoside transporter
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 63959
Homologene: 37985
Ccar2
Name: cell cycle activator and apoptosis regulator 2
Synonyms: 2610301G19Rik, Dbc1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219158
VEGA: 14
Homologene: 10910
Sh3glb1
Name: SH3-domain GRB2-like B1 (endophilin)
Synonyms: Endophilin B1, Bif-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54673
Homologene: 9337
Cep350
Name: centrosomal protein 350
Synonyms: 4933409L06Rik, 6430546F08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74081
Homologene: 8879
Pld3
Name: phospholipase D family member 3
Synonyms: Sam-9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18807
Homologene: 7893
Atp13a3
Name: ATPase type 13A3
Synonyms: LOC224087, LOC224088, LOC385637
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224088
Homologene: 23455
Helq
Name: helicase, POLQ-like
Synonyms: D430018E21Rik, Hel308
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 191578
Homologene: 14667
Ncbp2
Name: nuclear cap binding protein subunit 2
Synonyms: 20kDa
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 68092
Homologene: 56377
Svil
Name: supervillin
Synonyms: B430302E16Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225115
Homologene: 25090
Dennd6a
Name: DENN domain containing 6A
Synonyms: Fam116a, A630054L15Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211922
Homologene: 13503
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269700
Homologene: 28297
Prss35
Name: serine protease 35
Synonyms: 6030424L22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244954
Homologene: 17734
Hpse
Name: heparanase
Synonyms: Hpa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15442
HGNC: HGNC:5164
Homologene: 68528
Ccr1
Name: C-C motif chemokine receptor 1
Synonyms: Cmkbr1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12768
VEGA: 9
HGNC: HGNC:1602
Homologene: 20344
Cdkl2
Name: cyclin dependent kinase like 2
Synonyms: KKIAMRE, Kkm, 5330436L21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53886
HGNC: HGNC:1782
Homologene: 55793
Kif27
Name: kinesin family member 27
Synonyms: 4930517I18Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75050
VEGA: 13
Homologene: 69235
Piezo2
Name: piezo-type mechanosensitive ion channel component 2
Synonyms: Piezo2, Fam38b, 9030411M15Rik, Fam38b2, 9430028L06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 667742
Homologene: 49695
Setd1b
Name: SET domain containing 1B
Synonyms: KMT2G
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208043
Homologene: 134654
Synm
Name: synemin, intermediate filament protein
Synonyms: Synemin, 4930412K21Rik, Dmn
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233335
Homologene: 9081
Chtf18
Name: CTF18, chromosome transmission fidelity factor 18
Synonyms: CTF18, 6030457M03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 214901
Homologene: 32532
Serpina3g
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3G
Synonyms: alpha-1 antiproteinase, Spi2A, alpha-1 antiproteinase,, Spi2-1, 2A2, Spi2/eb.1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20715
HGNC: HGNC:16
Homologene: 115927
Dennd5a
Name: DENN domain containing 5A
Synonyms: 1500012B19Rik, ORF37, Rab6ip1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19347
Homologene: 14584
Spag17
Name: sperm associated antigen 17
Synonyms: PF6, 4931427F14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74362
Homologene: 52601
Vmn2r108
Name: vomeronasal 2, receptor 108
Synonyms: EG627805
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627805
Homologene: 129678
Grid2
Name: glutamate receptor, ionotropic, delta 2
Synonyms: B230104L07Rik, GluRdelta2, tpr
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14804
HGNC: HGNC:4576
Homologene: 74399
Dut
Name: deoxyuridine triphosphatase
Synonyms: 5031412I06Rik, 5133400F09Rik, Dutp, dUTPase, D2Bwg0749e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 110074
HGNC: HGNC:3078
Homologene: 31475
Rimbp3
Name: RIMS binding protein 3
Synonyms: LOC385766, LOC239731, RIM-BP3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239731
Homologene: 77940
Or14c45
Name: olfactory receptor family 14 subfamily C member 45
Synonyms: Olfr297, MOR220-3, GA_x6K02T2NHDJ-9587747-9586815
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258611
Homologene: 115526
Catsperg1
Name: cation channel sperm associated auxiliary subunit gamma 1
Synonyms: Catsperg, A230107C01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 320225
Homologene: 10915
Iigp1c
Name: interferon inducible GTPase 1C
Synonyms: Gm4951
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240327
Csnka2ip
Name: casein kinase 2, alpha prime interacting protein
Synonyms: Ckt2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224291
Homologene: 77570
Gcm1
Name: glial cells missing homolog 1
Synonyms: glial cell deficient, Gcm 1, Gcm a, Gcm1-rs1, glide, GCMa
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14531
VEGA: 9
HGNC: HGNC:4197
Homologene: 2702
Cbx2
Name: chromobox 2
Synonyms: M33
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12416
HGNC: HGNC:1552
Homologene: 7256
Mtmr11
Name: myotubularin related protein 11
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 194126
Homologene: 82382
Kcnj15
Name: potassium inwardly-rectifying channel, subfamily J, member 15
Synonyms: Kir4.2, IRKK, 4930414N08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16516
HGNC: HGNC:6261
Homologene: 1690
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 155,862,154 bp
  • T to C, chromosome 2 at 125,251,019 bp
  • G to A, chromosome 3 at 90,172,068 bp
  • A to T, chromosome 3 at 96,171,188 bp
  • A to T, chromosome 3 at 100,103,132 bp
  • T to C, chromosome 3 at 144,705,605 bp
  • T to A, chromosome 5 at 92,033,217 bp
  • G to A, chromosome 5 at 100,695,569 bp
  • A to T, chromosome 5 at 100,768,384 bp
  • T to A, chromosome 5 at 121,334,375 bp
  • G to A, chromosome 5 at 123,161,799 bp
  • A to T, chromosome 6 at 64,345,593 bp
  • T to C, chromosome 7 at 27,537,731 bp
  • T to C, chromosome 7 at 29,206,567 bp
  • A to G, chromosome 7 at 67,734,966 bp
  • GCCTCCTCCTCCTCCTCCTCCTCCTCCTCC to GCCTCCTCCTCCTCCTCCTCCTCCTCC, chromosome 7 at 80,512,904 bp
  • T to A, chromosome 7 at 86,527,102 bp
  • A to G, chromosome 7 at 109,894,666 bp
  • T to C, chromosome 9 at 78,059,783 bp
  • G to T, chromosome 9 at 86,755,653 bp
  • A to G, chromosome 9 at 123,964,106 bp
  • T to G, chromosome 10 at 20,278,233 bp
  • T to A, chromosome 11 at 119,027,926 bp
  • T to C, chromosome 12 at 104,239,082 bp
  • C to A, chromosome 13 at 58,345,716 bp
  • T to A, chromosome 14 at 26,629,534 bp
  • T to A, chromosome 14 at 70,143,069 bp
  • A to T, chromosome 16 at 17,212,929 bp
  • A to G, chromosome 16 at 30,361,869 bp
  • CGTCTGGATG to CG, chromosome 16 at 31,956,343 bp
  • G to A, chromosome 16 at 64,479,381 bp
  • C to T, chromosome 16 at 95,296,259 bp
  • A to T, chromosome 17 at 20,472,347 bp
  • A to G, chromosome 17 at 25,721,244 bp
  • A to T, chromosome 17 at 45,589,245 bp
  • A to C, chromosome 18 at 5,057,323 bp
  • A to G, chromosome 18 at 60,245,768 bp
  • G to T, chromosome 18 at 63,086,607 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6446 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044583-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.