Strain Name:
C57BL/6J-MtgxR6532Btlr/Mmmh
Stock Number:
044658-MU
Citation ID:
RRID:MMRRC_044658-MU
Other Names:
R6532 (G1)
Major Collection:

Strain Information

Nlk
Name: nemo like kinase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18099
Homologene: 88836
Atr
Name: ataxia telangiectasia and Rad3 related
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245000
HGNC: HGNC:882
Homologene: 96916
Nvl
Name: nuclear VCP-like
Synonyms: 1200009I24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67459
HGNC: HGNC:8070
Homologene: 1902
Tdrd3
Name: tudor domain containing 3
Synonyms: 4732418C03Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219249
Homologene: 12771
Hook1
Name: hook microtubule tethering protein 1
Synonyms: azh, abnormal spermatozoon head shape, A930033L17Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 77963
Homologene: 9289
Unc5b
Name: unc-5 netrin receptor B
Synonyms: 6330415E02Rik, D10Bwg0792e, Unc5h2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 107449
VEGA: 10
Homologene: 32538
Pnkp
Name: polynucleotide kinase 3'- phosphatase
Synonyms: 1810009G08Rik, PNK
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 59047
HGNC: HGNC:9154
Homologene: 5247
Garnl3
Name: GTPase activating RANGAP domain-like 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99326
Homologene: 13003
Dpp9
Name: dipeptidylpeptidase 9
Synonyms: DPRP2, 6430584G11Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224897
VEGA: 17
Homologene: 16385
Exoc1
Name: exocyst complex component 1
Synonyms: Sec3p, Sec3l1, 2810407P21Rik, A730011E05Rik, SEC3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69940
Homologene: 41241
Akap2
Name: A kinase (PRKA) anchor protein 2
Synonyms: B230340M18Rik, AKAP-KL
Type: Gene
Species: Mouse
Chromosome: 4
Skic2
Name: SKI2 subunit of superkiller complex
Synonyms: Ski2w, 4930534J06Rik, Skiv2l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 108077
Homologene: 123971
Hoxb7
Name: homeobox B7
Synonyms: Hox-2.3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15415
HGNC: HGNC:5118
Homologene: 55828
Cubn
Name: cubilin
Synonyms: D2Wsu88e, intrinsic factor-cobalamin receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: b2b1554Clo, A2mr, CD91
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Lama4
Name: laminin, alpha 4
Synonyms: laminin [a]4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16775
HGNC: HGNC:6484
Homologene: 37604
Adal
Name: adenosine deaminase-like
Synonyms: 4930578F03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75894
Homologene: 13827
Hoxa7
Name: homeobox A7
Synonyms: Hox-1.1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 15404
HGNC: HGNC:5108
Homologene: 56001
Haspin
Name: histone H3 associated protein kinase
Synonyms: Gsg2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14841
Homologene: 49236
Ifi207
Name: interferon activated gene 207
Synonyms: AI607873, Pyhin-A
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226691
HGNC: HGNC:5395
Homologene: 115929
Abcc6
Name: ATP-binding cassette, sub-family C member 6
Synonyms: Dyscalc1, Mrp6, DCC
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27421
HGNC: HGNC:57
Homologene: 55559
Myo1f
Name: myosin IF
Synonyms: C330006B10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17916
HGNC: HGNC:7600
Homologene: 56276
Pcnx1
Name: pecanex 1
Synonyms: Pcnx, 3526401J03Rik, 2900024E21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 54604
VEGA: 12
Homologene: 40997
Idh3b
Name: isocitrate dehydrogenase 3 (NAD+) beta
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 170718
HGNC: HGNC:5385
Homologene: 5035
Fam227a
Name: family with sequence similarity 227, member A
Synonyms: 4933432B09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75729
Homologene: 123434
Kcnt2
Name: potassium channel, subfamily T, member 2
Synonyms: E330038N15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240776
Homologene: 16121
Zfp563
Name: zinc finger protein 563
Synonyms: Zfp413, zinc finger protein
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240068
Homologene: 77346
Or9m1
Name: olfactory receptor family 9 subfamily M member 1
Synonyms: Olfr1154, GA_x6K02T2Q125-49403456-49402524, MOR173-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258641
Homologene: 74192
Glb1l3
Name: galactosidase, beta 1 like 3
Synonyms: 4921509F24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70893
Homologene: 72240
Slc25a54
Name: solute carrier family 25, member 54
Synonyms: SCaMC-1like, 4930443G12Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74686
Homologene: 82464
Inca1
Name: inhibitor of CDK, cyclin A1 interacting protein 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103844
Homologene: 19481
Wfdc2
Name: WAP four-disulfide core domain 2
Synonyms: 1600023A02Rik, HE4, WAP5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67701
Homologene: 4450
Ear1
Name: eosinophil-associated, ribonuclease A family, member 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13586
Homologene: 40596
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 140,584,106 bp
  • A to G, chromosome 1 at 173,729,645 bp
  • G to A, chromosome 1 at 181,144,143 bp
  • T to C, chromosome 2 at 13,459,002 bp
  • A to T, chromosome 2 at 33,031,119 bp
  • A to T, chromosome 2 at 87,903,202 bp
  • A to G, chromosome 2 at 121,146,123 bp
  • AG to AGCACCACAACTG, chromosome 2 at 130,279,673 bp
  • A to G, chromosome 2 at 164,563,376 bp
  • A to G, chromosome 3 at 109,112,052 bp
  • T to C, chromosome 4 at 57,855,174 bp
  • T to G, chromosome 4 at 96,019,756 bp
  • T to C, chromosome 5 at 76,537,837 bp
  • A to G, chromosome 6 at 52,217,315 bp
  • T to A, chromosome 7 at 44,857,405 bp
  • A to G, chromosome 7 at 45,977,379 bp
  • T to C, chromosome 9 at 26,818,442 bp
  • T to A, chromosome 9 at 95,908,408 bp
  • G to A, chromosome 10 at 39,048,077 bp
  • A to G, chromosome 10 at 60,778,828 bp
  • A to C, chromosome 10 at 127,541,682 bp
  • A to G, chromosome 10 at 127,549,407 bp
  • T to C, chromosome 11 at 70,695,907 bp
  • A to T, chromosome 11 at 73,137,551 bp
  • G to T, chromosome 11 at 78,696,055 bp
  • C to A, chromosome 11 at 96,286,888 bp
  • C to A, chromosome 12 at 81,980,964 bp
  • C to T, chromosome 14 at 43,819,252 bp
  • A to G, chromosome 14 at 87,505,816 bp
  • C to T, chromosome 15 at 79,636,720 bp
  • A to G, chromosome 17 at 33,105,698 bp
  • A to G, chromosome 17 at 33,575,846 bp
  • G to A, chromosome 17 at 34,844,743 bp
  • C to T, chromosome 17 at 56,205,854 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6532 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044658-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.