Strain Name:
C57BL/6J-MtgxR6535Btlr/Mmmh
Stock Number:
044661-MU
Citation ID:
RRID:MMRRC_044661-MU
Other Names:
R6535 (G1)
Major Collection:

Strain Information

Apobec3
Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3
Synonyms: CEM15, Rfv3, Gm20117, Rfv-3
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 80287
Homologene: 105420
Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Chrm1
Name: cholinergic receptor, muscarinic 1, CNS
Synonyms: muscarinic acetylcholine receptor 1, M1, AW495047, M1R, Chrm-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12669
HGNC: HGNC:1950
Homologene: 20189
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Aclp7, trabeculin alpha, Acf7
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: nmf2, nur14, C630029C19Rik, seal, med, motor end-plate disease, ataxia 3, nmf335, mnd2, mnd-2, nmf58, NaCh6, NMF335, Nav1.6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20273
Homologene: 7927
Pi4ka
Name: phosphatidylinositol 4-kinase alpha
Synonyms: Pik4ca
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224020
HGNC: HGNC:8983
Homologene: 11171
Prrc2a
Name: proline-rich coiled-coil 2A
Synonyms: 3110039B05Rik, Bat-2, Bat2, G2, D17H6S51E, Wbp12
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 53761
Homologene: 10567
Hip1
Name: huntingtin interacting protein 1
Synonyms: E130315I21Rik, HIP-1, 2610109B09Rik, A930014B11Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 215114
HGNC: HGNC:4913
Homologene: 68463
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, AnkG, 2900054D09Rik, Ankyrin-3, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Cpt1a
Name: carnitine palmitoyltransferase 1a, liver
Synonyms: L-CPT I, Cpt1, CPTI
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12894
VEGA: 19
HGNC: HGNC:2328
Homologene: 1413
Pole
Name: polymerase (DNA directed), epsilon
Synonyms: pol-epsilon
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18973
HGNC: HGNC:9177
Homologene: 4539
Cpne6
Name: copine VI
Synonyms: neuronal copine
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12891
HGNC: HGNC:2319
Homologene: 81815
Mettl8
Name: methyltransferase 8, methylcytidine
Synonyms: TIP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228019
Homologene: 11706
Adgrf5
Name: adhesion G protein-coupled receptor F5
Synonyms: 8430401C09Rik, Gpr116
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224792
VEGA: 17
Homologene: 9065
Lama2
Name: laminin, alpha 2
Synonyms: merosin, mer
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Dennd6b
Name: DENN domain containing 6B
Synonyms: Fam116b, 1700027J05Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69440
VEGA: 15
Homologene: 57065
Mipol1
Name: mirror-image polydactyly 1
Synonyms: 6030439O22Rik, D12Ertd19e, 1700081O04Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 73490
Homologene: 16340
Sbk3
Name: SH3 domain binding kinase family, member 3
Synonyms: Gm1078, LOC381835
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381835
Homologene: 82595
Fam135b
Name: family with sequence similarity 135, member B
Synonyms: 1700010C24Rik, A830008O07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70363
VEGA: 15
Homologene: 66605
Ctsq
Name: cathepsin Q
Synonyms: 1600010J02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 104002
Homologene: 122226
Tshz3
Name: teashirt zinc finger family member 3
Synonyms: Tsh3, A630038G13Rik, teashirt3, Zfp537
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243931
Homologene: 10835
B4gat1
Name: beta-1,4-glucuronyltransferase 1
Synonyms: B3gnt6, BETA3GNT1, 1500032M01Rik, iGNT, B3gnt1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 108902
VEGA: 19
Homologene: 38239
Macrod1
Name: mono-ADP ribosylhydrolase 1
Synonyms: D930010J01Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107227
VEGA: 19
Homologene: 8549
Cers1
Name: ceramide synthase 1
Synonyms: Lass1, to, Uog-1, CerS1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93898
Homologene: 128762
Vmn1r88
Name: vomeronasal 1 receptor, 88
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100312474
Homologene: 74345
Rhbdl1
Name: rhomboid like 1
Synonyms: Rhbdl
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 214951
Homologene: 2946
Tcstv2c
Name: two cell stage variable group member 2C
Synonyms: Gm20767
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 639910
VEGA: 13
Ly6g6g
Name: lymphocyte antigen 6 family member G6G
Synonyms: D730001G18Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78725
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 2 at 70,973,389 bp
  • A to T, chromosome 4 at 123,471,935 bp
  • A to C, chromosome 5 at 110,324,807 bp
  • T to C, chromosome 5 at 135,428,497 bp
  • A to C, chromosome 7 at 4,969,841 bp
  • C to T, chromosome 7 at 13,178,185 bp
  • T to C, chromosome 7 at 36,768,789 bp
  • T to C, chromosome 8 at 33,336,103 bp
  • T to A, chromosome 8 at 70,330,154 bp
  • A to C, chromosome 10 at 27,104,131 bp
  • G to T, chromosome 10 at 69,877,854 bp
  • A to C, chromosome 12 at 57,306,100 bp
  • A to T, chromosome 13 at 61,035,326 bp
  • T to C, chromosome 13 at 120,154,654 bp
  • A to T, chromosome 14 at 55,513,665 bp
  • A to G, chromosome 15 at 71,622,075 bp
  • T to C, chromosome 15 at 74,772,225 bp
  • A to G, chromosome 15 at 79,897,749 bp
  • A to G, chromosome 15 at 89,186,367 bp
  • G to A, chromosome 15 at 100,959,707 bp
  • C to T, chromosome 16 at 17,301,036 bp
  • A to T, chromosome 17 at 25,835,825 bp
  • A to G, chromosome 17 at 35,162,265 bp
  • G to A, chromosome 17 at 43,440,029 bp
  • T to A, chromosome 19 at 3,365,788 bp
  • T to C, chromosome 19 at 5,039,530 bp
  • A to G, chromosome 19 at 7,057,147 bp
  • T to A, chromosome 19 at 8,679,073 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6535 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044661-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.