Strain Name:
C57BL/6J-MtgxR6549Btlr/Mmmh
Stock Number:
044674-MU
Citation ID:
RRID:MMRRC_044674-MU
Other Names:
R6549 (G1)
Major Collection:

Strain Information

Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 4921505C17Rik, D530039E11Rik, 6030405M08Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: NBCn1, NBC3, E430014N10Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Pus10
Name: pseudouridylate synthase 10
Synonyms: Ccdc139, 4933435A13Rik, 2810013G11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74467
Homologene: 12566
Cndp1
Name: carnosine dipeptidase 1
Synonyms: Cn1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 338403
Homologene: 57178
Piezo1
Name: piezo-type mechanosensitive ion channel component 1
Synonyms: Piezo1, Fam38a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234839
Homologene: 124356
Zfp988
Name: zinc finger protein 988
Synonyms: Gm13151
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 115489950
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: MDHC7, Dnahc1, B230373P09Rik, E030034C22Rik, ferf1, G1-415-19
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Scn4a
Name: sodium channel, voltage-gated, type IV, alpha
Synonyms: SkM1, mH2, Nav1.4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 110880
Homologene: 283
C1rl
Name: complement component 1, r subcomponent-like
Synonyms: C1r-LP, C1rl1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232371
Homologene: 9559
Primpol
Name: primase and polymerase (DNA-directed)
Synonyms: Ccdc111
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 408022
Homologene: 14065
Ldb3
Name: LIM domain binding 3
Synonyms: cypher, ZASP
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 24131
VEGA: 14
Homologene: 134626
Sox6
Name: SRY (sex determining region Y)-box 6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20679
Homologene: 22631
Sell
Name: selectin, lymphocyte
Synonyms: CD62L, Lyam-1, L-selectin, Lnhr, Ly-m22, Ly-22, Lyam1, LECAM-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20343
Homologene: 539
Scn2a
Name: sodium channel, voltage-gated, type II, alpha
Synonyms: A230052E19Rik, Scn2a1, Nav1.2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 110876
Homologene: 75001
Atxn7
Name: ataxin 7
Synonyms: A430107N12Rik, Sca7, ataxin-7
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 246103
VEGA: 14
Homologene: 30967
Vmn2r77
Name: vomeronasal 2, receptor 77
Synonyms: EG546983
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 546983
Homologene: 115466
Ccdc54
Name: coiled-coil domain containing 54
Synonyms: 1700007N18Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 69339
VEGA: 16
Homologene: 49871
Vmn1r31
Name: vomeronasal 1 receptor 31
Synonyms: Gm6709
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 626828
Homologene: 115643
Synj1
Name: synaptojanin 1
Synonyms: A930006D20Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 104015
Homologene: 48252
Gm9922
Name: predicted gene 9922
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 102633345
VEGA: 14
Or8k30
Name: olfactory receptor family 8 subfamily K member 30
Synonyms: MOR189-2, Olfr1076, GA_x6K02T2Q125-47993761-47994702
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258401
Homologene: 17233
Hycc2
Name: hyccin PI4KA lipid kinase complex subunit 2
Synonyms: Fam126b, D1Ertd53e
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 213056
Homologene: 18184
Col25a1
Name: collagen, type XXV, alpha 1
Synonyms: 2700062B08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77018
Homologene: 57111
Defb8
Name: defensin beta 8
Synonyms: mBD8, Defr1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244334
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 58,539,600 bp
  • A to G, chromosome 1 at 164,065,629 bp
  • A to G, chromosome 2 at 65,764,674 bp
  • A to T, chromosome 2 at 86,509,382 bp
  • A to G, chromosome 3 at 130,182,795 bp
  • G to A, chromosome 4 at 147,331,853 bp
  • A to T, chromosome 4 at 152,131,068 bp
  • A to T, chromosome 6 at 58,472,663 bp
  • T to A, chromosome 6 at 124,508,528 bp
  • A to G, chromosome 7 at 86,800,857 bp
  • A to G, chromosome 7 at 115,486,692 bp
  • G to T, chromosome 8 at 19,447,544 bp
  • A to T, chromosome 8 at 46,605,150 bp
  • G to A, chromosome 8 at 122,500,263 bp
  • T to C, chromosome 9 at 53,493,177 bp
  • G to A, chromosome 11 at 23,729,075 bp
  • T to C, chromosome 11 at 106,343,965 bp
  • T to C, chromosome 14 at 14,013,087 bp
  • T to A, chromosome 14 at 14,748,564 bp
  • A to T, chromosome 14 at 31,269,383 bp
  • A to G, chromosome 14 at 34,541,897 bp
  • C to A, chromosome 14 at 101,729,457 bp
  • C to G, chromosome 15 at 6,796,175 bp
  • T to C, chromosome 16 at 50,590,025 bp
  • A to G, chromosome 16 at 90,938,677 bp
  • A to T, chromosome 18 at 84,636,184 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6549 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044674-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.