Strain Name:
C57BL/6J-MtgxR6557Btlr/Mmmh
Stock Number:
044681-MU
Citation ID:
RRID:MMRRC_044681-MU
Other Names:
R6557 (G1)
Major Collection:

Strain Information

Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Sec24d
Name: SEC24 homolog D, COPII coat complex component
Synonyms: LOC383951, 2310020L09Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69608
Homologene: 40986
Zfp638
Name: zinc finger protein 638
Synonyms: Np220, Zfml
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18139
Homologene: 7447
Mon2
Name: MON2 homolog, regulator of endosome to Golgi trafficking
Synonyms: SF21, 2610528O22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67074
VEGA: 10
Homologene: 44309
Scaper
Name: S phase cyclin A-associated protein in the ER
Synonyms: D530014O03Rik, Zfp291
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244891
VEGA: 9
Homologene: 32488
Hip1
Name: huntingtin interacting protein 1
Synonyms: E130315I21Rik, HIP-1, 2610109B09Rik, A930014B11Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 215114
HGNC: HGNC:4913
Homologene: 68463
Lamb2
Name: laminin, beta 2
Synonyms: Lams, npht, Lamb-2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 16779
HGNC: HGNC:6487
Homologene: 1723
Ccn3
Name: cellular communication network factor 3
Synonyms: CCN3, C130088N23Rik, Nov
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18133
HGNC: HGNC:7885
Homologene: 1884
Zzz3
Name: zinc finger, ZZ domain containing 3
Synonyms: 6430567E01Rik, 3110065C23Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 108946
Homologene: 9182
Nfatc3
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3
Synonyms: D8Ertd281e, NFAT4, NFATx
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18021
HGNC: HGNC:7777
Homologene: 27827
Liph
Name: lipase, member H
Synonyms: PLA1B, mPA-PLA1, D16Wsu119e, C130037N08Rik, LPDLR, Lpdlr
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239759
VEGA: 16
Homologene: 71802
Ikzf3
Name: IKAROS family zinc finger 3
Synonyms: Zfpn1a3, Aiolos, 5830411O07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22780
Homologene: 8269
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
Tdrd5
Name: tudor domain containing 5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 214575
Homologene: 18312
Mamdc2
Name: MAM domain containing 2
Synonyms: 1200015L10Rik, mamcan
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71738
VEGA: 19
Homologene: 17722
Topaz1
Name: testis and ovary specific PAZ domain containing 1
Synonyms: Gm9524
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 671232
VEGA: 9
Homologene: 19835
Greb1
Name: gene regulated by estrogen in breast cancer protein
Synonyms: 5730583K22Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 268527
Homologene: 8780
Krtap16-1
Name: keratin associated protein 16-1
Synonyms: AI450886
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100504183
Homologene: 99988
Ica1l
Name: islet cell autoantigen 1-like
Synonyms: b2b3465Clo, Als2cr15, 1700030B17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70375
Homologene: 16323
Gpc5
Name: glypican 5
Synonyms: A230034F01Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 103978
HGNC: HGNC:4453
Homologene: 3285
Or4f52
Name: olfactory receptor family 4 subfamily F member 52
Synonyms: MOR245-18, GA_x6K02T2Q125-72283260-72282322, Olfr1275
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 257980
Homologene: 79419
Cxcl15
Name: C-X-C motif chemokine ligand 15
Synonyms: lungkine, Il8, weche, Scyb15
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20309
Hecw1
Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Synonyms: NEDL1, E130207I19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94253
VEGA: 13
Homologene: 9004
Map10
Name: microtubule-associated protein 10
Synonyms: 4933403G14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74393
Homologene: 10416
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 59,997,625 bp
  • T to C, chromosome 1 at 156,300,721 bp
  • T to C, chromosome 2 at 111,231,631 bp
  • T to C, chromosome 3 at 123,343,087 bp
  • T to A, chromosome 3 at 152,428,460 bp
  • C to T, chromosome 5 at 90,794,566 bp
  • T to C, chromosome 5 at 135,428,719 bp
  • C to A, chromosome 6 at 83,930,110 bp
  • A to G, chromosome 6 at 84,186,384 bp
  • T to C, chromosome 8 at 106,119,354 bp
  • T to C, chromosome 8 at 125,670,252 bp
  • T to C, chromosome 9 at 55,550,850 bp
  • T to C, chromosome 9 at 108,488,400 bp
  • A to G, chromosome 9 at 122,748,895 bp
  • C to A, chromosome 10 at 123,016,402 bp
  • C to T, chromosome 11 at 98,516,881 bp
  • T to C, chromosome 11 at 99,985,130 bp
  • T to C, chromosome 12 at 16,710,383 bp
  • T to C, chromosome 13 at 14,316,646 bp
  • A to T, chromosome 14 at 115,092,534 bp
  • A to T, chromosome 15 at 54,747,927 bp
  • T to A, chromosome 16 at 21,983,920 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • A to G, chromosome 19 at 23,310,845 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6557 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044681-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.