Strain Name:
C57BL/6J-MtgxR6577Btlr/Mmmh
Stock Number:
044701-MU
Citation ID:
RRID:MMRRC_044701-MU
Other Names:
R6577 (G1)
Major Collection:

Strain Information

Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66797
Homologene: 69159
Tns3
Name: tensin 3
Synonyms: TEM6, F830010I22Rik, Tens1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319939
Homologene: 49713
Ark2n
Name: arkadia (RNF111) N-terminal like PKA signaling regulator 2N
Synonyms: Ark2N, 8030462N17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 212163
VEGA: 18
Homologene: 17035
Hells
Name: helicase, lymphoid specific
Synonyms: PASG, YFK8, proliferation-associated SNF2-like, Lysh, LSH, E130115I21Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15201
HGNC: HGNC:4861
Homologene: 50037
Tut7
Name: terminal uridylyl transferase 7
Synonyms: 6030448M23Rik, Zcchc6, Tent3b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 214290
VEGA: 13
Homologene: 51941
Nup98
Name: nucleoporin 98
Synonyms: Nup96
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269966
HGNC: HGNC:8068
Homologene: 35472
Api5
Name: apoptosis inhibitor 5
Synonyms: AAC-11
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11800
HGNC: HGNC:594
Homologene: 4809
Dgkd
Name: diacylglycerol kinase, delta
Synonyms: dgkd-2, DGKdelta
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227333
HGNC: HGNC:2851
Homologene: 100054
Srp68
Name: signal recognition particle 68
Synonyms: 2610024I03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217337
Homologene: 7056
Def8
Name: differentially expressed in FDCP 8
Synonyms: D8Ertd713e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23854
Homologene: 14279
Asap3
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
Synonyms: UPLC1, Ddefl1, 9430088F20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230837
Homologene: 41190
Tmem120b
Name: transmembrane protein 120B
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 330189
Homologene: 62404
Gjc1
Name: gap junction protein, gamma 1
Synonyms: Cx45, Gja-7, C130009G16Rik, connexin 45, Gja7
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14615
HGNC: HGNC:4280
Homologene: 4016
Clec10a
Name: C-type lectin domain family 10, member A
Synonyms: CD301a, Mgl1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17312
Homologene: 7836
Tsc2
Name: TSC complex subunit 2
Synonyms: tuberous sclerosis 2, tuberin, Nafld
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22084
VEGA: 17
Homologene: 462
Ephb2
Name: Eph receptor B2
Synonyms: eteck, Nuk, Cek5, Qek5, Prkm5, Sek3, Erk, Hek5, Drt, Tyro5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13844
HGNC: HGNC:3393
Homologene: 37925
Cpsf1
Name: cleavage and polyadenylation specific factor 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 94230
VEGA: 15
HGNC: HGNC:2324
Homologene: 40865
Hc
Name: hemolytic complement
Synonyms: C5, He, Hfib2, C5a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15139
HGNC: HGNC:1331
Homologene: 20412
Atp11b
Name: ATPase, class VI, type 11B
Synonyms: 1110019I14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76295
Homologene: 32919
Irf6
Name: interferon regulatory factor 6
Synonyms: E230028I05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54139
HGNC: HGNC:6121
Homologene: 4479
Atp13a4
Name: ATPase type 13A4
Synonyms: 9330174J19Rik, 4631413J11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224079
Homologene: 75330
Kcnv2
Name: potassium channel, subfamily V, member 2
Synonyms: KV11.1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240595
Homologene: 26423
Scml4
Name: Scm polycomb group protein like 4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268297
Homologene: 18262
Myo1a
Name: myosin IA
Synonyms: BBM-I, brush border myosin 1, Myhl
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 432516
VEGA: 10
HGNC: HGNC:7595
Homologene: 21113
Ppfibp1
Name: PTPRF interacting protein, binding protein 1 (liprin beta 1)
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67533
HGNC: HGNC:9249
Homologene: 2685
Papolg
Name: poly(A) polymerase gamma
Synonyms: 9630006B20Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216578
Homologene: 56959
Hid1
Name: HID1 domain containing
Synonyms: C630004H02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217310
Homologene: 12737
Zfp820
Name: zinc finger protein 820
Synonyms: 2610036F08Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 75424
VEGA: 17
Srsf12
Name: serine and arginine-rich splicing factor 12
Synonyms: Srrp, Sfrs13b
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 272009
Lmln
Name: leishmanolysin-like (metallopeptidase M8 family)
Synonyms: 5330415H22Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239833
Homologene: 13198
Sardh
Name: sarcosine dehydrogenase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192166
Homologene: 5149
Stat5b
Name: signal transducer and activator of transcription 5B
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20851
Homologene: 55718
Upb1
Name: ureidopropionase, beta
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103149
Homologene: 9471
Uba1y
Name: ubiquitin-activating enzyme, Chr Y
Synonyms: Ube1y-1, Sby, Ube1y1, Ube-2, A1s9Y-1
Type: Gene
Species: Mouse
Chromosome: Y
NCBI: 22202
Homologene: 129435
Skap1
Name: src family associated phosphoprotein 1
Synonyms: Skap-55, 1700091G21Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78473
Homologene: 2764
Cracd
Name: capping protein inhibiting regulator of actin
Synonyms: C530008M17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320827
Homologene: 136278
Cd27
Name: CD27 antigen
Synonyms: Tnfrsf7, Cd27, S152, Tp55
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21940
Homologene: 74386
Uros
Name: uroporphyrinogen III synthase
Synonyms: uroporphyrinogen-III synthase, Uros3, URO-synthase, hydroxymethylbilane hydrolyase (cyclizing)
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22276
Homologene: 37296
Tma7
Name: translational machinery associated 7
Synonyms: 1110017O22Rik, Ccdc72
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66167
Homologene: 41078
Igkv8-24
Name: immunoglobulin kappa chain variable 8-24
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 677858
HGNC: HGNC:5834
Arl14
Name: ADP-ribosylation factor-like 14
Synonyms: 9130014L17Rik, Arf7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71619
Homologene: 11812
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 87,940,240 bp
  • A to G, chromosome 1 at 193,169,354 bp
  • T to C, chromosome 2 at 27,218,855 bp
  • T to C, chromosome 2 at 35,032,126 bp
  • T to C, chromosome 2 at 94,422,381 bp
  • T to A, chromosome 3 at 35,839,162 bp
  • A to G, chromosome 3 at 69,223,072 bp
  • T to C, chromosome 4 at 33,209,196 bp
  • T to C, chromosome 4 at 136,238,230 bp
  • T to C, chromosome 4 at 136,657,550 bp
  • A to T, chromosome 5 at 76,866,100 bp
  • T to G, chromosome 5 at 123,116,647 bp
  • C to T, chromosome 6 at 46,170,272 bp
  • C to T, chromosome 6 at 70,216,963 bp
  • T to C, chromosome 6 at 125,236,793 bp
  • T to A, chromosome 6 at 146,999,655 bp
  • A to C, chromosome 7 at 102,128,846 bp
  • C to A, chromosome 7 at 133,700,840 bp
  • T to C, chromosome 8 at 123,456,710 bp
  • T to C, chromosome 9 at 109,082,194 bp
  • A to G, chromosome 10 at 42,947,111 bp
  • T to G, chromosome 10 at 75,412,889 bp
  • A to T, chromosome 10 at 127,715,320 bp
  • G to A, chromosome 11 at 8,549,057 bp
  • G to T, chromosome 11 at 8,549,058 bp
  • GGACTTGGGATACTTACGCTTTG to GG, chromosome 11 at 23,879,857 bp
  • T to C, chromosome 11 at 70,170,610 bp
  • T to C, chromosome 11 at 96,526,044 bp
  • A to G, chromosome 11 at 100,797,700 bp
  • T to C, chromosome 11 at 102,800,304 bp
  • G to C, chromosome 11 at 115,354,636 bp
  • G to A, chromosome 11 at 116,265,464 bp
  • C to T, chromosome 13 at 59,808,161 bp
  • CCCCTGCATGAGGCAGGTCCC to CCCC, chromosome 15 at 76,597,455 bp
  • C to T, chromosome 16 at 29,479,841 bp
  • T to A, chromosome 16 at 33,107,000 bp
  • T to C, chromosome 17 at 21,819,403 bp
  • C to T, chromosome 17 at 24,610,499 bp
  • T to C, chromosome 18 at 77,653,159 bp
  • T to A, chromosome 19 at 27,324,020 bp
  • C to T, chromosome 19 at 38,931,465 bp
  • A to G, chromosome Y at 825,465 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6577 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044701-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.