Strain Name:
C57BL/6J-MtgxR6588Btlr/Mmmh
Stock Number:
044712-MU
Citation ID:
RRID:MMRRC_044712-MU
Other Names:
R6588 (G1)
Major Collection:

Strain Information

Kitl
Name: kit ligand
Synonyms: stem cell factor, Sl, grizzle-belly, Steel factor, SLF, SF, Mgf, Gb, SCF, Kitlg, blz, Steel
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: HALR, E330008K23Rik, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Macroh2a1
Name: macroH2A.1 histone
Synonyms: MACROH2A1.2, H2afy, H2AF12M, mH2a1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26914
HGNC: HGNC:4740
Homologene: 3598
Lsm3
Name: LSM3 homolog, U6 small nuclear RNA and mRNA degradation associated
Synonyms: SMX4, USS2, 1010001J12Rik, 2610005D18Rik, 6030401D18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67678
Homologene: 6548
Urb2
Name: URB2 ribosome biogenesis 2 homolog (S. cerevisiae)
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382038
Homologene: 8859
Mms19
Name: MMS19 cytosolic iron-sulfur assembly component
Synonyms: 2610042O15Rik, C86341, Mms19, Mms19l
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72199
Homologene: 41480
Zfp64
Name: zinc finger protein 64
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22722
Homologene: 7604
Erc1
Name: ELKS/RAB6-interacting/CAST family member 1
Synonyms: RAB6IP2B, RAB6IP2A, Rab6ip2, 9630025C19Rik, 5033405M01Rik, Elks1, B430107L16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 111173
Homologene: 14229
Itfg1
Name: integrin alpha FG-GAP repeat containing 1
Synonyms: 2310047C21Rik, D8Wsu49e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71927
Homologene: 12423
Ghr
Name: growth hormone receptor
Synonyms: GHBP, GHR/BP
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14600
HGNC: HGNC:4263
Homologene: 134
Clcn3
Name: chloride channel, voltage-sensitive 3
Synonyms: Clc3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12725
HGNC: HGNC:2021
Homologene: 20435
Cpsf1
Name: cleavage and polyadenylation specific factor 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 94230
VEGA: 15
HGNC: HGNC:2324
Homologene: 40865
Lrrd1
Name: leucine rich repeats and death domain containing 1
Synonyms: 4932412H11Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 242838
Homologene: 18247
Bean1
Name: brain expressed, associated with Nedd4, 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 65115
Homologene: 110172
2210408I21Rik
Name: RIKEN cDNA 2210408I21 gene
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72371
Homologene: 89234
Fhl4
Name: four and a half LIM domains 4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14202
VEGA: 10
Or10aa1
Name: olfactory receptor family 10 subfamily AA member 1
Synonyms: Olfr433, GA_x6K02T2P20D-21133014-21132070, MOR123-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 258712
Homologene: 133588
Or7e173
Name: olfactory receptor family 7 subfamily E member 173
Synonyms: MOR145-5, GA_x6K02T2PVTD-13768406-13767468, Olfr866
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258551
HGNC: HGNC:8396
Homologene: 133687
Or1o1
Name: olfactory receptor family 1 subfamily O member 1
Synonyms: Olfr107, GA_x6K02T2PSCP-1867165-1868094, MOR156-3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258504
Homologene: 115498
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 174,042,278 bp
  • A to T, chromosome 2 at 168,926,907 bp
  • A to G, chromosome 5 at 3,851,386 bp
  • C to T, chromosome 5 at 25,323,789 bp
  • GATATATA to GATATATATA, chromosome 6 at 91,519,635 bp
  • C to T, chromosome 6 at 119,575,726 bp
  • T to C, chromosome 8 at 60,914,827 bp
  • T to C, chromosome 8 at 85,736,130 bp
  • CT to C, chromosome 8 at 104,182,032 bp
  • G to T, chromosome 8 at 124,031,125 bp
  • G to T, chromosome 9 at 20,027,866 bp
  • A to G, chromosome 10 at 85,098,107 bp
  • T to A, chromosome 10 at 100,064,092 bp
  • T to A, chromosome 12 at 115,980,751 bp
  • C to T, chromosome 13 at 56,104,489 bp
  • T to C, chromosome 13 at 77,192,647 bp
  • T to C, chromosome 15 at 3,320,268 bp
  • A to G, chromosome 15 at 76,596,822 bp
  • G to A, chromosome 17 at 37,405,905 bp
  • C to T, chromosome 19 at 41,966,276 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6588 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044712-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.