Strain Name:
C57BL/6J-MtgxR6656Btlr/Mmmh
Stock Number:
044777-MU
Citation ID:
RRID:MMRRC_044777-MU
Other Names:
R6656 (G1)
Major Collection:

Strain Information

Tmem63b
Name: transmembrane protein 63b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224807
Homologene: 101682
Ptpn3
Name: protein tyrosine phosphatase, non-receptor type 3
Synonyms: 9530011I20Rik, PTPCL, PTP-H1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 545622
HGNC: HGNC:9655
Homologene: 74451
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: B630009I04Rik, Helic1, ASC1p200
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Anln
Name: anillin, actin binding protein
Synonyms: 1110037A17Rik, Scraps, 2900037I21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68743
VEGA: 9
Homologene: 41281
Ahctf1
Name: AT hook containing transcription factor 1
Synonyms: Elys, 6230412P20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226747
Homologene: 9142
Orc2
Name: origin recognition complex, subunit 2
Synonyms: Orc2l
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18393
HGNC: HGNC:8488
Homologene: 4512
Angptl1
Name: angiopoietin-like 1
Synonyms: ANGPT3, ANG3, ANG-3, ANG3, ARP1, 2810039D03Rik, ANGY
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72713
HGNC: HGNC:489
Homologene: 128467
Eif2ak3
Name: eukaryotic translation initiation factor 2 alpha kinase 3
Synonyms: PERK
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13666
HGNC: HGNC:3255
Homologene: 3557
Sec63
Name: SEC63 homolog, protein translocation regulator
Synonyms: 5730478J10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 140740
Homologene: 5220
Bhlhe40
Name: basic helix-loop-helix family, member e40
Synonyms: eip1 (E47 interaction protein 1), DEC1, cytokine response gene 8, Stra13, CR8, Bhlhb2, Clast5, C130042M06Rik, Sharp2, Stra14
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20893
HGNC: HGNC:1046
Homologene: 2722
Cd22
Name: CD22 antigen
Synonyms: Lyb8, Lyb-8
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12483
HGNC: HGNC:1643
Homologene: 31052
Muc5ac
Name: mucin 5, subtypes A and C, tracheobronchial/gastric
Synonyms: MGM, 2210005L13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17833
HGNC: HGNC:7515
Homologene: 137237
Gngt1
Name: guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
Synonyms: G(y)1, Gng1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14699
HGNC: HGNC:4411
Homologene: 7738
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Ahnak2
Name: AHNAK nucleoprotein 2
Synonyms: LOC382643
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100041194
Homologene: 131081
Cyp2b19
Name: cytochrome P450, family 2, subfamily b, polypeptide 19
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13090
HGNC: HGNC:2615
Homologene: 104114
Ift140
Name: intraflagellar transport 140
Synonyms: Tce5, Wdtc2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106633
Homologene: 40979
Keg1
Name: kidney expressed gene 1
Synonyms: 0610008P16Rik, GS4059
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 64697
Homologene: 69461
B3galnt2
Name: UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 2
Synonyms: D230016N13Rik, A930105D20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 97884
VEGA: 13
Homologene: 17595
Mctp1
Name: multiple C2 domains, transmembrane 1
Synonyms: 2810465F10Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 78771
Homologene: 75211
Parpbp
Name: PARP1 binding protein
Synonyms: 4930547N16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 75317
Homologene: 49519
Sirpd
Name: signal regulatory protein delta
Synonyms: Gm9733
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 751864
Homologene: 135594
Sgip1
Name: SH3-domain GRB2-like (endophilin) interacting protein 1
Synonyms: 3110007P09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 73094
Homologene: 13001
Or13a21
Name: olfactory receptor family 13 subfamily A member 21
Synonyms: Olfr532, MOR251-1, GA_x6K02T2PBJ9-42570051-42569122
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259028
Homologene: 133605
Myb
Name: myeloblastosis oncogene
Synonyms: c-myb
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17863
HGNC: HGNC:7545
Homologene: 31311
Ehf
Name: ets homologous factor
Synonyms: 9030625L19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13661
HGNC: HGNC:3246
Homologene: 7301
Piwil4
Name: piwi-like RNA-mediated gene silencing 4
Synonyms: Miwi2, MIWI2, 9230101H05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330890
Homologene: 45122
D930048N14Rik
Name: RIKEN cDNA D930048N14 gene
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 97775
Tktl2
Name: transketolase-like 2
Synonyms: 4933401I19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74419
Homologene: 69456
Lyz3
Name: lysozyme 3
Synonyms: 9530003J23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77397
HGNC: HGNC:6740
Homologene: 49931
Npas2
Name: neuronal PAS domain protein 2
Synonyms: bHLHe9, MOP4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18143
HGNC: HGNC:7895
Homologene: 1887
Fbxo4
Name: F-box protein 4
Synonyms: 1700096C12Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 106052
Homologene: 8134
Pcdha3
Name: protocadherin alpha 3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 192163
HGNC: HGNC:8669
Homologene: 129613
Pramel51
Name: PRAME like 51
Synonyms: Gm10436
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100039315
VEGA: 12
Homologene: 103830
Gm21190
Name: predicted gene, 21190
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100861755
Homologene: 69402
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 39,361,948 bp
  • A to G, chromosome 1 at 58,493,659 bp
  • A to T, chromosome 1 at 156,857,236 bp
  • T to C, chromosome 1 at 179,753,513 bp
  • A to T, chromosome 2 at 40,637,864 bp
  • C to T, chromosome 2 at 76,709,356 bp
  • T to C, chromosome 2 at 103,283,583 bp
  • T to C, chromosome 3 at 15,320,498 bp
  • T to A, chromosome 4 at 57,205,905 bp
  • C to A, chromosome 4 at 102,905,568 bp
  • T to C, chromosome 5 at 15,525,851 bp
  • A to G, chromosome 6 at 3,994,246 bp
  • T to A, chromosome 6 at 70,883,715 bp
  • TG to TGG, chromosome 6 at 108,664,857 bp
  • C to T, chromosome 7 at 26,766,855 bp
  • T to C, chromosome 7 at 30,877,757 bp
  • G to T, chromosome 7 at 140,419,604 bp
  • A to G, chromosome 7 at 141,803,328 bp
  • T to C, chromosome 8 at 66,512,729 bp
  • T to C, chromosome 9 at 14,709,934 bp
  • A to T, chromosome 9 at 22,351,002 bp
  • C to T, chromosome 10 at 21,152,945 bp
  • C to T, chromosome 10 at 42,816,383 bp
  • C to T, chromosome 10 at 50,649,925 bp
  • T to C, chromosome 10 at 88,110,313 bp
  • A to G, chromosome 10 at 117,235,629 bp
  • C to A, chromosome 11 at 51,653,749 bp
  • A to T, chromosome 12 at 88,175,993 bp
  • C to T, chromosome 12 at 112,785,371 bp
  • C to T, chromosome 13 at 13,975,576 bp
  • A to T, chromosome 13 at 77,029,936 bp
  • C to T, chromosome 15 at 3,975,823 bp
  • T to C, chromosome 17 at 25,032,173 bp
  • C to A, chromosome 17 at 45,667,708 bp
  • T to A, chromosome 18 at 12,549,226 bp
  • T to C, chromosome 18 at 36,947,822 bp
  • C to T, chromosome 19 at 12,709,630 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6656 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044777-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.