Strain Name:
C57BL/6J-MtgxR7255Btlr/Mmmh
Stock Number:
045316-MU
Citation ID:
RRID:MMRRC_045316-MU
Other Names:
R7255 (G1)
Major Collection:

Strain Information

Slc1a3
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: Gmt1, Eaat1, B430115D02Rik, MGluT1, GLAST
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20512
Homologene: 20882
Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228852
Homologene: 9194
Cd86
Name: CD86 antigen
Synonyms: MB7-2, Ly58, Cd28l2, B70, B7.2, B7-2, Ly-58
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12524
HGNC: HGNC:1705
Homologene: 10443
Inpp5a
Name: inositol polyphosphate-5-phosphatase A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 212111
HGNC: HGNC:6076
Homologene: 4045
Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: BB001228, 2510010B09Rik, Cxxc6, D10Ertd17e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52463
Homologene: 12735
Dop1b
Name: DOP1 leucine zipper like protein B
Synonyms: 2610510B01Rik, 0610038M01Rik, Dopey2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70028
VEGA: 16
HGNC: HGNC:1291
Homologene: 21068
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: Aprin, AS3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100710
Homologene: 41001
Spats1
Name: spermatogenesis associated, serine-rich 1
Synonyms: Srsp1, 1700011H05Rik, 4933400B06Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 71020
VEGA: 17
Homologene: 12376
Bcat1
Name: branched chain aminotransferase 1, cytosolic
Synonyms: BCATc, Bcat-1, Eca39
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12035
HGNC: HGNC:976
Homologene: 20320
Tcf7l1
Name: transcription factor 7 like 1 (T cell specific, HMG box)
Synonyms: Tcf3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21415
Homologene: 7563
Dip2b
Name: disco interacting protein 2 homolog B
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239667
Homologene: 72227
Sulf1
Name: sulfatase 1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240725
Homologene: 49408
Prcc
Name: papillary renal cell carcinoma (translocation-associated)
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 94315
HGNC: HGNC:9343
Homologene: 38120
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: Sh3d5, Ponsin, c-Cbl-associated protein, CAP, 9530001P15Rik, 2310065E01Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Trrap
Name: transformation/transcription domain-associated protein
Synonyms: transactivation/transformation-domain associated protein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100683
Homologene: 39246
Slc25a25
Name: solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 25
Synonyms: 1110030N17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227731
Homologene: 62165
Myo9b
Name: myosin IXb
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17925
HGNC: HGNC:7609
Homologene: 3058
Wdr26
Name: WD repeat domain 26
Synonyms: Gid7, 1600024A01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226757
Homologene: 11857
Syne1
Name: spectrin repeat containing, nuclear envelope 1
Synonyms: enaptin165, nesprin-1, A330049M09Rik, C130039F11Rik, SYNE-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64009
VEGA: 10
Homologene: 52329
Cyb5r3
Name: cytochrome b5 reductase 3
Synonyms: Dia1, 2500002N19Rik, Dia-1, 0610016L08Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 109754
HGNC: HGNC:2873
Homologene: 47921
Zfp160
Name: zinc finger protein 160
Synonyms: 6720480D16Rik, 6720480D16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224585
VEGA: 17
Homologene: 137372
Sdhb
Name: succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Synonyms: 0710008N11Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67680
Homologene: 2255
Ipo9
Name: importin 9
Synonyms: Imp9, 0710008K06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226432
Homologene: 5874
Dnajc8
Name: DnaJ heat shock protein family (Hsp40) member C8
Synonyms: 2010009J04Rik, 1110021D09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68598
Homologene: 40935
Nefm
Name: neurofilament, medium polypeptide
Synonyms: NF160, neurofilament-M, Nef3, NF165, NF-M, Nfm
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18040
HGNC: HGNC:7734
Homologene: 137211
Mfsd2a
Name: MFSD2 lysolipid transporter A, lysophospholipid
Synonyms: Mfsd2, major facilitator superfamily domain containing 2A, 1700018O18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76574
Homologene: 19229
Pamr1
Name: peptidase domain containing associated with muscle regeneration 1
Synonyms: E430002G05Rik, RAMP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 210622
Homologene: 9134
Casp2
Name: caspase 2
Synonyms: Caspase-2, Nedd2, Ich-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12366
HGNC: HGNC:1503
Homologene: 7254
Arhgap28
Name: Rho GTPase activating protein 28
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268970
Homologene: 18264
Rfx7
Name: regulatory factor X, 7
Synonyms: 2510005N23Rik, D130086K05Rik, Rfxdc2, 9930116O05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319758
Homologene: 11275
Uggt1
Name: UDP-glucose glycoprotein glucosyltransferase 1
Synonyms: A930007H10Rik, C820010P03Rik, Ugcgl1, 0910001L17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 320011
Homologene: 10586
Vps13a
Name: vacuolar protein sorting 13A
Synonyms: 4930543C13Rik, D330038K10Rik, 4930516E05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 271564
VEGA: 19
HGNC: HGNC:1908
Homologene: 22068
Ssh2
Name: slingshot protein phosphatase 2
Synonyms: SSH-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237860
Homologene: 14116
Plxna2
Name: plexin A2
Synonyms: OCT, Plxn2, 2810428A13Rik, PlexA2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Enpp1
Name: ectonucleotide pyrophosphatase/phosphodiesterase 1
Synonyms: Pca, Pca-1, E-NPP1, CD203c, NPP1, twy, 4833416E15Rik, PC-1, Ly-41, Npps, Pdnp1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18605
HGNC: HGNC:3356
Homologene: 38151
Atp8b1
Name: ATPase, class I, type 8B, member 1
Synonyms: Ic, FIC1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 54670
VEGA: 18
HGNC: HGNC:3706
Homologene: 21151
Btbd16
Name: BTB domain containing 16
Synonyms: E330040A16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330660
Homologene: 77327
Myo16
Name: myosin XVI
Synonyms: BM140241, Nyap3, C230040D10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244281
Homologene: 34710
Cpsf1
Name: cleavage and polyadenylation specific factor 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 94230
VEGA: 15
HGNC: HGNC:2324
Homologene: 40865
Slc5a8
Name: solute carrier family 5 (iodide transporter), member 8
Synonyms: SMCT
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216225
VEGA: 10
Homologene: 64832
Sema6b
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B
Synonyms: Seman, Sema, VIb, semaZ
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20359
VEGA: 17
Homologene: 8428
Flt1
Name: FMS-like tyrosine kinase 1
Synonyms: Flt-1, VEGFR1, sFlt1, vascular endothelial growth factor receptor-1, VEGFR-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14254
HGNC: HGNC:3763
Homologene: 134179
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 545030
Homologene: 83473
Dock10
Name: dedicator of cytokinesis 10
Synonyms: A630054M16Rik, Zizimin3, Jr5, Jr4, ZIZ3, 9330153B10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210293
Homologene: 45952
Shkbp1
Name: Sh3kbp1 binding protein 1
Synonyms: SB1, B930062H15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 192192
Homologene: 26700
Slco1c1
Name: solute carrier organic anion transporter family, member 1c1
Synonyms: Slc21a14, OATP-F
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 58807
Homologene: 23008
Flnc
Name: filamin C, gamma
Synonyms: 1110055E19Rik, actin binding protein 280, Fln2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 68794
HGNC: HGNC:3756
Homologene: 37481
Asic1
Name: acid-sensing ion channel 1
Synonyms: ASIC1b, B530003N02Rik, Accn2, BNaC2, ASICalpha, ASIC1a, ASIC, ASIC1 beta
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11419
VEGA: 15
HGNC: HGNC:100
Homologene: 121755
Shpk
Name: sedoheptulokinase
Synonyms: 4930431K22Rik, Carkl
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74637
HGNC: HGNC:1492
Homologene: 8319
Aldh1a7
Name: aldehyde dehydrogenase family 1, subfamily A7
Synonyms: Ahd2-like, Aldh-pb
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 26358
HGNC: HGNC:402
Homologene: 137245
Psg19
Name: pregnancy specific beta-1-glycoprotein 19
Synonyms: Cea-4, Cea4, CGM7
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26439
HGNC: HGNC:1819
Homologene: 110989
Tsks
Name: testis-specific serine kinase substrate
Synonyms: Tssks1, Stk22s1, Tsks, clone 4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22116
Homologene: 7984
Sanbr
Name: SANT and BTB domain regulator of CSR
Synonyms: 0610010F05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71675
Homologene: 19038
Galc
Name: galactosylceramidase
Synonyms: galactocerebrosidase, A930008M05Rik, Gacy, 2310068B06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14420
VEGA: 12
HGNC: HGNC:4115
Homologene: 124
Fcna
Name: ficolin A
Synonyms: Fcn1, ficolin A
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14133
Homologene: 117949
Or10ak11
Name: olfactory receptor family 10 subfamily AK member 11
Synonyms: MOR259-11, MOR259-6, Olfr1333, GA_x6K02T2QD9B-18703033-18703986
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 258265
Homologene: 134082
Gbp2b
Name: guanylate binding protein 2b
Synonyms: Gbp1, Gbp-1, Mag-1, Mpa1, Mpa-1, LIMIT
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14468
Homologene: 137233
Rgl2
Name: ral guanine nucleotide dissociation stimulator-like 2
Synonyms: Rlf, KE1.5, Rgt2, Rab2l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19732
HGNC: HGNC:9769
Homologene: 3494
Dsc1
Name: desmocollin 1
Synonyms: Dsc1b, Dsc1a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13505
VEGA: 18
HGNC: HGNC:3035
Homologene: 22761
Tlr5
Name: toll-like receptor 5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 53791
Homologene: 20698
Lyz3
Name: lysozyme 3
Synonyms: 9530003J23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77397
HGNC: HGNC:6740
Homologene: 49931
Or10ag2
Name: olfactory receptor family 10 subfamily AG member 2
Synonyms: MOR264-17, GA_x6K02T2Q125-48917235-48918206, Olfr1123
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258347
Homologene: 105187
Crisp4
Name: cysteine-rich secretory protein 4
Synonyms: 9230112K08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78081
HGNC: HGNC:304
Homologene: 81683
Med7
Name: mediator complex subunit 7
Synonyms: Crsp9, Crsp33, 1110063B05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66213
HGNC: HGNC:2378
Homologene: 3153
Klra4
Name: killer cell lectin-like receptor, subfamily A, member 4
Synonyms: Ly49d, Chok, ly49r129
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16635
Homologene: 110821
Lag3
Name: lymphocyte-activation gene 3
Synonyms: Ly66, CD223, LAG-3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16768
HGNC: HGNC:6476
Homologene: 1719
Sbspon
Name: somatomedin B and thrombospondin, type 1 domain containing
Synonyms: LOC226866, Gm106
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226866
Homologene: 45950
Mup9
Name: major urinary protein 9
Synonyms: Gm14076
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100038948
Homologene: 74304
Pnrc1
Name: proline-rich nuclear receptor coactivator 1
Synonyms: B4-2, PRR2, 5730463C12Rik, Prol2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 108767
Homologene: 4960
Krtap10-29
Name: keratin associated protein 10-29
Synonyms: Gm9639
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 675294
VEGA: 10
Homologene: 115744
Gm8297
Name: predicted gene 8297
Type: Gene
Species: Mouse
Chromosome: 14
Homologene: 115686
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 12,859,008 bp
  • G to T, chromosome 1 at 15,883,797 bp
  • C to A, chromosome 1 at 18,130,231 bp
  • T to C, chromosome 1 at 36,146,106 bp
  • C to T, chromosome 1 at 80,543,099 bp
  • T to C, chromosome 1 at 135,385,988 bp
  • A to C, chromosome 1 at 181,181,324 bp
  • T to C, chromosome 1 at 182,974,316 bp
  • T to G, chromosome 1 at 194,752,103 bp
  • T to G, chromosome 2 at 25,626,028 bp
  • T to C, chromosome 2 at 32,421,372 bp
  • T to A, chromosome 2 at 87,418,942 bp
  • T to C, chromosome 2 at 102,611,584 bp
  • T to C, chromosome 2 at 158,761,391 bp
  • A to T, chromosome 3 at 87,870,091 bp
  • T to A, chromosome 3 at 142,608,117 bp
  • C to T, chromosome 4 at 33,248,045 bp
  • A to G, chromosome 4 at 60,421,337 bp
  • A to T, chromosome 4 at 118,829,952 bp
  • A to C, chromosome 4 at 122,952,021 bp
  • A to G, chromosome 4 at 132,551,573 bp
  • A to G, chromosome 4 at 140,977,418 bp
  • T to C, chromosome 5 at 144,858,954 bp
  • C to T, chromosome 5 at 147,580,406 bp
  • T to A, chromosome 5 at 150,796,667 bp
  • G to T, chromosome 6 at 29,445,766 bp
  • A to G, chromosome 6 at 42,268,907 bp
  • A to T, chromosome 6 at 72,627,347 bp
  • A to G, chromosome 6 at 124,910,235 bp
  • A to G, chromosome 6 at 130,059,642 bp
  • A to G, chromosome 6 at 141,569,325 bp
  • C to A, chromosome 6 at 145,032,785 bp
  • A to G, chromosome 7 at 18,794,048 bp
  • G to T, chromosome 7 at 27,342,748 bp
  • C to T, chromosome 7 at 44,952,688 bp
  • A to T, chromosome 7 at 130,785,992 bp
  • A to G, chromosome 7 at 139,511,448 bp
  • A to T, chromosome 8 at 10,499,169 bp
  • T to C, chromosome 8 at 71,290,891 bp
  • T to G, chromosome 9 at 72,619,828 bp
  • A to G, chromosome 10 at 5,333,446 bp
  • A to T, chromosome 10 at 24,645,315 bp
  • A to T, chromosome 10 at 62,822,636 bp
  • G to A, chromosome 10 at 77,794,538 bp
  • A to T, chromosome 10 at 88,909,631 bp
  • T to C, chromosome 10 at 117,234,422 bp
  • G to A, chromosome 11 at 23,620,465 bp
  • T to A, chromosome 11 at 46,440,995 bp
  • A to G, chromosome 11 at 73,199,660 bp
  • T to A, chromosome 11 at 77,425,593 bp
  • T to C, chromosome 12 at 98,246,255 bp
  • T to A, chromosome 14 at 4,984,874 bp
  • A to G, chromosome 14 at 32,974,282 bp
  • A to G, chromosome 14 at 68,116,000 bp
  • A to G, chromosome 15 at 8,642,999 bp
  • G to A, chromosome 15 at 76,597,543 bp
  • A to G, chromosome 15 at 83,160,165 bp
  • A to G, chromosome 15 at 99,697,457 bp
  • G to A, chromosome 15 at 100,209,627 bp
  • CA to CAA, chromosome 16 at 36,606,555 bp
  • C to A, chromosome 16 at 93,770,146 bp
  • T to A, chromosome 17 at 21,025,487 bp
  • T to C, chromosome 17 at 33,934,990 bp
  • T to A, chromosome 17 at 45,454,205 bp
  • T to C, chromosome 17 at 56,125,336 bp
  • T to C, chromosome 17 at 67,853,004 bp
  • C to T, chromosome 18 at 20,097,273 bp
  • T to A, chromosome 18 at 64,556,868 bp
  • A to G, chromosome 19 at 16,654,339 bp
  • A to G, chromosome 19 at 20,714,728 bp
  • G to C, chromosome 19 at 40,376,800 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7255 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045316-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.