Strain Name:
FVB.Cg-OsPlm-Tg(Pgk1)2Ddp/WiseMmjax
Stock Number:
067449-JAX
Citation ID:
RRID:MMRRC_067449-JAX
Other Names:
94-K; PlmTgN(Pgk1)2/Ddp

Strain Information

OsPlm-Tg(Pgk1)2Ddp
Name: postimplantation lethal mutation induced by Pgk1 transgene insertion-Dimitrina D Pravtcheva 2
Synonyms: 94-K, OsPlmTg(Pgk1)2Ddp
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Transgenic (random, gene disruption)
Pgk1
Name: phosphoglycerate kinase 1
Synonyms: Pgk-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Transgenic (random, gene disruption)
NCBI: 18655
HGNC: HGNC:8896
Homologene: 21542
Anapc10
Name: anaphase promoting complex subunit 10
Synonyms: Apc10, 1500026N15Rik, A830003M23Rik
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Transgenic (random, gene disruption)
NCBI: 68999
Homologene: 32238
Genetic Alterations

94-K mice have a Pgk1 transgene insertion that has disrupted the Anapc10 gene.

Phenotype
Homozygotes die early in embryogenesis due to a mitotic block caused by the disruption of the Anapc10 gene, which encodes a component of the anaphase-promoting complex.
MeSH Terms
  • Abnormalities, Radiation-Induced/genetics
  • Alleles
  • Amino Acid Sequence
  • Animals
  • Apc10 Subunit, Anaphase-Promoting Complex-Cyclosome
  • Base Sequence
  • Cell Cycle Proteins/chemistry
  • Cell Cycle Proteins/genetics
  • Cell Cycle Proteins/physiology
  • Chromosomes, Artificial, Bacterial
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression
  • Gene Rearrangement
  • Humans
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Mutation
  • Saccharomyces cerevisiae Proteins
  • Sequence Deletion
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic
  • Transgenes
  • Gene Expression Regulation, Developmental
  • Mice, Transgenic/embryology
  • Mitosis/genetics
  • Chromosome Mapping
  • Chromosomes
  • Cricetinae
  • Crosses, Genetic
  • Female
  • Genes, Lethal/genetics
  • Homozygote
  • Karyotyping
  • Male
  • Mice, Inbred C57BL
  • Mutagenesis, Insertional
  • Phosphoglycerate Kinase/genetics
  • Time Factors
  • Transgenes/genetics
Strain Development
The pCAla construct used for embryo microinjection contained an insert of approximately 26 kb, including the entire Pgk1 gene, plus 5 kb of 5' and 2.8kb of 3' flanking sequences, This construct was injected into B6D2F1 X CD1 embryos. Founder 94 underwent a rearrangement to generate two lines, 94A and 94K, both of these lines were shown subsequently to have disrupted the Anapc10 locus. 94K mice have been backcrossed to FVB/N mice for >20 generations.
Suggested Control Mice
FVB/N
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Models for Human Disease
Donor
Thomas Wise, Ph.D., NYS Institute for Basic Research in Developmental Disabilities.
Primary Reference

Pravtcheva DD, Wise TL. Disruption of Apc10/Doc1 in three alleles of oligosyndactylism. Genomics. 2001 Feb 15;72(1):78-87. doi: 10.1006/geno.2001.6474. (Medline PMID: 11247669)

Pravtcheva DD, Wise TL. A transgene-induced mitotic arrest mutation in the mouse allelic with Oligosyndactylism. Genetics. 1996 Dec;144(4):1747-56. (Medline PMID: 8978060)

Pravtcheva DD, Wise TL. A postimplantation lethal mutation induced by transgene insertion on mouse chromosome 8. Genomics. 1995 Dec 10;30(3):529-44. doi: 10.1006/geno.1995.1274. (Medline PMID: 8825640)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
White
Eye
Pink
MMRRC Breeding System
Backcross
Generation
N20+ (FVB/N)
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months Greater than 12 months
Bred to Homozygosity
Yes
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067449-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
067449-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.