Strain Name:
C57BL/6J-MtgxR8229Btlr/Mmmh
Stock Number:
067645-MU
Citation ID:
RRID:MMRRC_067645-MU
Other Names:
R8229 (G1)
Major Collection:

Strain Information

Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: E430025J12Rik, Nedd4-1, Nedd4, Nedd4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Gli1
Name: GLI-Kruppel family member GLI1
Synonyms: Zfp-5, Zfp5
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14632
VEGA: 10
HGNC: HGNC:4317
Homologene: 3859
Sipa1l1
Name: signal-induced proliferation-associated 1 like 1
Synonyms: 4931426N11Rik, Spar
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217692
VEGA: 12
Homologene: 9189
Prepl
Name: prolyl endopeptidase-like
Synonyms: 2810457N15Rik, 9530014L06Rik, D030028O16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 213760
VEGA: 17
Homologene: 15481
Zfp609
Name: zinc finger protein 609
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214812
Homologene: 72220
Klhl24
Name: kelch-like 24
Synonyms: 1110046J11Rik, 6530402O20Rik, 4930429H24Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 75785
VEGA: 16
Homologene: 32352
Iws1
Name: IWS1, SUPT6 interacting protein
Synonyms: 1700069O15Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 73473
Homologene: 134421
Erc1
Name: ELKS/RAB6-interacting/CAST family member 1
Synonyms: RAB6IP2B, RAB6IP2A, Rab6ip2, 9630025C19Rik, 5033405M01Rik, Elks1, B430107L16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 111173
Homologene: 14229
Arhgap23
Name: Rho GTPase activating protein 23
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 58996
Homologene: 104145
Smc6
Name: structural maintenance of chromosomes 6
Synonyms: 3830418C19Rik, 2810489L22Rik, Smc6l1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67241
VEGA: 12
Homologene: 41575
Ermard
Name: ER membrane associated RNA degradation
Synonyms: 2410011O22Rik, 2210404J11Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381062
Homologene: 19936
D6Wsu163e
Name: DNA segment, Chr 6, Wayne State University 163, expressed
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28040
HGNC: HGNC:1184
Homologene: 10685
Magi3
Name: membrane associated guanylate kinase, WW and PDZ domain containing 3
Synonyms: 4732496O19Rik, 6530407C02Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99470
Homologene: 26431
Trim11
Name: tripartite motif-containing 11
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 94091
Homologene: 14225
Usp33
Name: ubiquitin specific peptidase 33
Synonyms: 9830169D19Rik, Vdu1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 170822
Homologene: 8996
Ythdc1
Name: YTH domain containing 1
Synonyms: A730098D12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231386
Homologene: 15796
Mgat5b
Name: mannoside acetylglucosaminyltransferase 5, isoenzyme B
Synonyms: GnT-IX
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268510
Homologene: 27821
Lrrc8c
Name: leucine rich repeat containing 8 family, member C
Synonyms: E430036I04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100604
Homologene: 12997
Ttll7
Name: tubulin tyrosine ligase-like family, member 7
Synonyms: 1110049N09Rik, 4921517B04Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70892
Homologene: 41578
Pcdhb11
Name: protocadherin beta 11
Synonyms: PcdhbK, Pcdhb5E
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93882
HGNC: HGNC:8690
Homologene: 62178
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Def6
Name: differentially expressed in FDCP 6
Synonyms: IBP, 2410003F05Rik, IRF-4-binding protein, 6430538D02Rik, SLAT
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 23853
HGNC: HGNC:2760
Homologene: 11103
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
Ywhab
Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
Synonyms: 1300003C17Rik, 14-3-3 beta, 14-3-3b
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 54401
Homologene: 20724
Or5b101
Name: olfactory receptor family 5 subfamily B member 101
Synonyms: GA_x6K02T2RE5P-3357666-3356743, MOR202-6, Olfr1453
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258695
HGNC: HGNC:8324
Homologene: 120159
Spty2d1
Name: SPT2 chromatin protein domain containing 1
Synonyms: 5830435K17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101685
Homologene: 45499
Lrrn4
Name: leucine rich repeat neuronal 4
Synonyms: B430119L13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320974
Homologene: 17632
Zscan30
Name: zinc finger and SCAN domain containing 30
Synonyms: Zfp397os, C230097I24Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 328918
VEGA: 18
Homologene: 48191
Inf2
Name: inverted formin, FH2 and WH2 domain containing
Synonyms: EG629699, 2610204M08Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 70435
VEGA: 12
Homologene: 82406
Or4c114
Name: olfactory receptor family 4 subfamily C member 114
Synonyms: MOR233-6, GA_x6K02T2Q125-50555603-50554668, Olfr1219
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258901
Homologene: 27292
Otud4
Name: OTU domain containing 4
Synonyms: D8Ertd69e, 4930431L18Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 73945
Homologene: 35370
Or5p57
Name: olfactory receptor family 5 subfamily P member 57
Synonyms: Olfr480, MOR204-32, GA_x6K02T2PBJ9-10395807-10394869
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56861
Homologene: 72039
Adam3
Name: ADAM metallopeptidase domain 3
Synonyms: tMDC, Cyrn1, Taz83, Taz83, ADAM3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11497
HGNC: HGNC:209
Homologene: 69052
Or5w17
Name: olfactory receptor family 5 subfamily W member 17
Synonyms: Olfr1141, MOR177-10, GA_x6K02T2Q125-49257818-49256883
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258630
Homologene: 74080
Ugt3a1
Name: UDP glycosyltransferases 3 family, polypeptide A1
Synonyms: Ugt3a2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223337
Homologene: 122787
H2-M10.1
Name: histocompatibility 2, M region locus 10.1
Synonyms: 9.5H
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14985
Homologene: 117973
Gm6034
Name: predicted gene 6034
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 547347
Phf11b
Name: PHD finger protein 11B
Synonyms: Gm4902
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 236451
Homologene: 87807
Pcdha7
Name: protocadherin alpha 7
Synonyms: Crnr4, Cnr4
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12939
HGNC: HGNC:8673
Homologene: 117962
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 82,978,143 bp
  • A to T, chromosome 2 at 87,753,064 bp
  • T to A, chromosome 2 at 89,075,038 bp
  • G to A, chromosome 2 at 132,869,887 bp
  • T to G, chromosome 2 at 164,014,095 bp
  • C to A, chromosome 3 at 104,015,701 bp
  • T to C, chromosome 3 at 104,015,702 bp
  • T to A, chromosome 3 at 146,901,449 bp
  • T to C, chromosome 3 at 152,370,292 bp
  • A to G, chromosome 5 at 67,028,795 bp
  • A to G, chromosome 5 at 86,809,308 bp
  • T to A, chromosome 5 at 105,606,536 bp
  • T to C, chromosome 6 at 119,753,288 bp
  • G to T, chromosome 6 at 126,967,003 bp
  • A to G, chromosome 7 at 46,997,774 bp
  • C to T, chromosome 7 at 108,066,587 bp
  • A to T, chromosome 8 at 24,711,738 bp
  • A to G, chromosome 8 at 79,673,975 bp
  • T to C, chromosome 9 at 65,703,500 bp
  • G to T, chromosome 9 at 72,731,388 bp
  • C to T, chromosome 10 at 127,332,448 bp
  • G to A, chromosome 11 at 58,981,341 bp
  • G to A, chromosome 11 at 97,453,906 bp
  • A to T, chromosome 11 at 116,947,387 bp
  • T to A, chromosome 12 at 11,291,672 bp
  • T to C, chromosome 12 at 82,437,848 bp
  • A to G, chromosome 12 at 112,611,596 bp
  • A to T, chromosome 14 at 59,331,281 bp
  • A to G, chromosome 15 at 9,367,377 bp
  • T to C, chromosome 16 at 20,114,571 bp
  • T to A, chromosome 17 at 15,059,334 bp
  • A to G, chromosome 17 at 28,217,755 bp
  • A to G, chromosome 17 at 36,056,376 bp
  • T to C, chromosome 17 at 36,324,039 bp
  • T to C, chromosome 17 at 85,081,261 bp
  • C to T, chromosome 18 at 12,407,551 bp
  • A to C, chromosome 18 at 23,971,680 bp
  • A to G, chromosome 18 at 32,084,687 bp
  • C to A, chromosome 18 at 36,974,723 bp
  • A to G, chromosome 18 at 37,422,618 bp
  • A to G, chromosome 19 at 13,028,197 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8229 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067645-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.