Strain Name:
C57BL/6J-MtgxR8232Btlr/Mmmh
Stock Number:
067664-MU
Citation ID:
RRID:MMRRC_067664-MU
Other Names:
R8232 (G1)
Major Collection:

Strain Information

Myo10
Name: myosin X
Synonyms: D15Ertd600e, myosin-X
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Nf1
Name: neurofibromin 1
Synonyms: Mhdadsk9, neurofibromin, Nf-1, Dsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Gtf2h1
Name: general transcription factor II H, polypeptide 1
Synonyms: p62, 62kDa
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14884
HGNC: HGNC:4655
Homologene: 3885
Arhgap32
Name: Rho GTPase activating protein 32
Synonyms: p200RhoGAP, PX-RICS, 3426406O18Rik, Grit, GC-GAP
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330914
Homologene: 8812
Bmp1
Name: bone morphogenetic protein 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12153
VEGA: 14
HGNC: HGNC:1067
Homologene: 55955
Vps50
Name: VPS50 EARP/GARPII complex subunit
Synonyms: Ccdc132, 1700034M03Rik, 8430415E05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73288
Homologene: 11498
Scyl2
Name: SCY1-like 2 (S. cerevisiae)
Synonyms: CVAK104, D10Ertd802e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 213326
Homologene: 13349
Bcr
Name: BCR activator of RhoGEF and GTPase
Synonyms: 5133400C09Rik, breakpoint cluster region
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 110279
VEGA: 10
HGNC: HGNC:1014
Homologene: 3192
Tmem183a
Name: transmembrane protein 183A
Synonyms: 1300007B12Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 57439
Homologene: 10757
Trhde
Name: TRH-degrading enzyme
Synonyms: 9330155P21Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237553
Homologene: 75007
Gcgr
Name: glucagon receptor
Synonyms: GR
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14527
HGNC: HGNC:4192
Homologene: 131
Camta2
Name: calmodulin binding transcription activator 2
Synonyms: Kiaa0909-hp
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216874
Homologene: 9021
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: Dnahc9, D11Ertd686e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Or6c202
Name: olfactory receptor family 6 subfamily C member 202
Synonyms: Olfr771, MOR114-8, GA_x6K02T2PULF-10846420-10845467
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258540
Homologene: 74047
Or4a72
Name: olfactory receptor family 4 subfamily A member 72
Synonyms: GA_x6K02T2Q125-51020951-51020028, Olfr1245, MOR231-12
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258784
Homologene: 121560
Adamts16
Name: ADAM metallopeptidase with thrombospondin type 1 motif 16
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 271127
Homologene: 15146
Or51ai2
Name: olfactory receptor family 51 subfamily AI member 2
Synonyms: Olfr632, GA_x6K02T2PBJ9-6671256-6672209, MOR2-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259123
Homologene: 64963
Ccnd2
Name: cyclin D2
Synonyms: 2600016F06Rik, Vin-1, Vin1, cD2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12444
HGNC: HGNC:1583
Homologene: 37525
Or5b107
Name: olfactory receptor family 5 subfamily B member 107
Synonyms: GA_x6K02T2RE5P-3491834-3492772, MOR202-35, Olfr1461
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258299
HGNC: HGNC:8324
Homologene: 128112
Il12b
Name: interleukin 12b
Synonyms: Il-12p40, IL-23 subunit p40, IL-12 p40, Il-12b
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16160
HGNC: HGNC:5970
Homologene: 1648
Actn3
Name: actinin alpha 3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 11474
HGNC: HGNC:165
Homologene: 862
Smarcal1
Name: SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1
Synonyms: 6030401P21Rik, Mharp
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54380
Homologene: 8558
Vmn2r58
Name: vomeronasal 2, receptor 58
Synonyms: EG628422
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 628422
Clip1
Name: CAP-GLY domain containing linker protein 1
Synonyms: Clip 170, Clip50, CLIP-170, 4631429H07Rik, cytoplasmic linker protein 50, Rsn, restin, 1110007I12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56430
Homologene: 74455
Or5p68
Name: olfactory receptor family 5 subfamily P member 68
Synonyms: MOR204-35, Olfr493, GA_x6K02T2PBJ9-10676998-10676054
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258307
Homologene: 17188
Nlrp5
Name: NLR family, pyrin domain containing 5
Synonyms: Op1, Mater, Nalp5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23968
Homologene: 65105
Acnat1
Name: acyl-coenzyme A amino acid N-acyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230161
Homologene: 28287
Gnl1
Name: guanine nucleotide binding protein-like 1
Synonyms: Gna-rs1, Gnal1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14670
HGNC: HGNC:4413
Homologene: 3862
Vmn1r229
Name: vomeronasal 1 receptor 229
Synonyms: V1re1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171224
Homologene: 74319
Kcnrg
Name: potassium channel regulator
Synonyms: E030012H22Rik, LOC328424
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 328424
VEGA: 14
Homologene: 35259
Nppa
Name: natriuretic peptide type A
Synonyms: ANP, natriuretic peptide precursor A, Anf, atrial natriuretic peptide, Pnd
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230899
HGNC: HGNC:7939
Homologene: 4498
Krtap4-6
Name: keratin associated protein 4-6
Synonyms: 1110054P19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68768
Homologene: 124486
Gm5930
Name: predicted gene 5930
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 546250
9430020K01Rik
Name:
Type: Gene
Species: Mouse
Chromosome: 18
Gm626
Name:
Type: Gene
Species: Mouse
Chromosome: 14
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 72,626,563 bp
  • T to A, chromosome 1 at 134,350,180 bp
  • A to T, chromosome 2 at 89,575,594 bp
  • A to T, chromosome 4 at 49,450,817 bp
  • T to C, chromosome 4 at 148,001,338 bp
  • G to C, chromosome 5 at 123,647,918 bp
  • T to G, chromosome 6 at 3,600,139 bp
  • A to G, chromosome 6 at 127,150,586 bp
  • G to T, chromosome 7 at 23,417,345 bp
  • A to T, chromosome 7 at 41,864,652 bp
  • T to A, chromosome 7 at 46,801,679 bp
  • G to A, chromosome 7 at 103,937,773 bp
  • A to T, chromosome 7 at 108,346,288 bp
  • A to T, chromosome 9 at 32,256,902 bp
  • A to G, chromosome 10 at 75,166,051 bp
  • A to T, chromosome 10 at 89,662,447 bp
  • A to T, chromosome 10 at 114,800,537 bp
  • C to A, chromosome 10 at 129,160,228 bp
  • A to G, chromosome 11 at 44,408,574 bp
  • A to T, chromosome 11 at 65,855,323 bp
  • G to A, chromosome 11 at 70,683,015 bp
  • A to G, chromosome 11 at 79,578,331 bp
  • A to C, chromosome 11 at 99,665,742 bp
  • A to G, chromosome 11 at 120,536,502 bp
  • C to A, chromosome 13 at 70,793,098 bp
  • T to G, chromosome 14 at 33,539,767 bp
  • T to G, chromosome 14 at 44,335,724 bp
  • T to A, chromosome 14 at 61,607,937 bp
  • T to A, chromosome 14 at 70,519,889 bp
  • G to A, chromosome 15 at 25,804,314 bp
  • T to A, chromosome 17 at 20,815,047 bp
  • T to A, chromosome 17 at 35,987,595 bp
  • G to T, chromosome 18 at 4,674,862 bp
  • A to T, chromosome 19 at 4,871,655 bp
  • A to G, chromosome 19 at 13,165,319 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8232 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067664-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.